| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
We identified dozens of novel interactor candidates of human growth hormone (GH) using our improved yeast two-hybrid method, and focused our efforts on the analyses of one of them, referred to here as “protein X”. We first confirmed that protein X is important for GH secretion in cells. We further found that protein X, expressed in E. coli, can interact with GH in vitro. The region containing the GH interaction surface of protein X was delineated within a 93-aa region. It has been reported that protein X can also interact with other hormones than GH, but our mutational analyses have revealed that the structure of protein-X interaction surface for GH is different from those for other hormones. The results therefore could provide a basis for designing inhibitor peptides that specifically deteriorate the GH-protein X interaction.
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