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Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis

Research Project

Project/Area Number 24791529
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionKanazawa University

Principal Investigator

WATANABE Koji  金沢大学, 医学系, 協力研究員 (90584839)

Co-Investigator(Renkei-kenkyūsha) MATSUBARA Hidenori  金沢大学, 医学系, 助教 (10507057)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords自己脂肪由来幹細胞 / 体性幹細胞 / 骨再生 / 骨延長 / 骨成熟促進
Research Abstract

The question of this study was whether uncultured adipose-derived regenerative cells(ADRCs), which can easily be isolated from autologous adipose tissue and contain several types of stem and regenerative cells, promote bone formation in distraction osteogenesis. Sixty rats were randomly assigned to 3 groups. Physiological saline (control group), Type I collagen gel, or a mixture of ADRC and Type I collagen gel (ADRC group) was injected into the distracted callus immediately after distraction termination.
The bone density of the distracted callus in the ADRC group increased by 46% compared with the control group at 6 weeks after injection. The fracture strength in the ADRC group increased by 66% compared with the control group at 6 weeks after injection. Real-time PCR of the distracted callus from the ADRC group had higher levels of BMP-2, VEGF A, and SDF-1. Cell labeling in the newly formed bone showed the ADRCs differentiated into osseous tissue at 3 weeks after injection.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (27 results)

All 2014 2013 2012 Other

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (18 results) Remarks (1 results)

  • [Journal Article] Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis in Rats2014

    • Author(s)
      Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya
    • Journal Title

      Clin Orthop Relat Res

      Volume: (In press)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Uncultured Autogenous Adipose-derived Regenerative Cells Promote Bone Formation During Distraction Osteogenesis in Rats.2014

    • Author(s)
      Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya.
    • Journal Title

      Clin Orthop Relat Res

      Volume: 印刷中

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Over 10-year follow-up of functional outcome in patients with bone tumors reconstructed using distraction osteogenesis.2012

    • Author(s)
      Watanabe K
    • Journal Title

      J Orthop Sci

      Volume: 18(1) Issue: 1 Pages: 101-109

    • DOI

      10.1007/s00776-012-0327-4

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Salvage surgery with a tumor prosthesis for femoral condylar nonunion at the very advanced age of 90 years: a case report.2012

    • Author(s)
      Watanabe K, Matsubara H, Nomura I, Tsuchiya H
    • Journal Title

      Formosan Journal of Musculoskeletal Disorders

      Volume: 3(4) Pages: 132-136

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 骨折部周囲への最小侵襲固定術の骨癒合期間の検討2012

    • Author(s)
      渡邊孝治, 松原秀憲, 野村一世, 土屋弘行
    • Journal Title

      日創外固定骨延長会誌

      Volume: 23 Pages: 89-92

    • NAID

      40021309138

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 第2MTP関節脱臼を伴った外反母趾の手術成績2012

    • Author(s)
      渡邊孝治, 松原秀憲, 野村一世, 土屋弘行
    • Journal Title

      日足外会誌

      Volume: 33 Pages: 137-40

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Taylor Spatial Frameの矯正精度―脛骨変形矯正症例での評価―2012

    • Author(s)
      渡邊孝治, 松原秀憲, 野村一世, 土屋弘行
    • Journal Title

      臨整外

      Volume: 47 Pages: 803-8

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 骨折部周囲への最小侵襲固定術の骨癒合期間の検討2012

    • Author(s)
      渡邊孝治, 松原秀憲, 野村一世, 土屋弘行
    • Journal Title

      別冊整形外

      Volume: 61 Pages: 12-8

    • NAID

      40021309138

    • Related Report
      2012 Research-status Report
  • [Presentation] Adipose-derived regenerative cells promote bone formation on distraction osteogenesis in rats2014

    • Author(s)
      I. Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya
    • Organizer
      Annual Meeting of the American Academy of Orthopaedic Surgeons
    • Place of Presentation
      USA
    • Year and Date
      2014-03-24
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する2014

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      日本創外固定・骨延長学会
    • Place of Presentation
      大阪
    • Year and Date
      2014-03-07
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来間葉系細胞移植による骨延長部骨形成促進法の開発2013

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      東京
    • Year and Date
      2013-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する2013

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      第32回日本運動器移植・再生医学研究会
    • Place of Presentation
      神戸
    • Year and Date
      2013-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] Autogenous adipose-derived regenerative cells promote bone formation on distraction osteogenesis in rats2013

    • Author(s)
      I. Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya
    • Organizer
      Twenty-third Annual Scientific Meeting.Limb Lengthening and Reconstruction Society: ASAMI-North America
    • Place of Presentation
      USA
    • Year and Date
      2013-07-19
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する2013

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      第120回中部日本整形外科災害外科学会・学術集会
    • Place of Presentation
      和歌山
    • Year and Date
      2013-04-05
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する2013

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      日本生体電気・物理刺激研究会
    • Place of Presentation
      京都
    • Year and Date
      2013-03-08
    • Related Report
      2013 Final Research Report
  • [Presentation] Adipose-derived regenerative cells promote bone formation following distraction osteogenesis in rats2012

    • Author(s)
      I. Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya
    • Organizer
      7th International ASAMI and Bone Reconstruction Meeting
    • Place of Presentation
      Greece
    • Year and Date
      2012-05-23
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来間葉系幹細胞移植による仮骨延長部骨形成の促進2012

    • Author(s)
      野村一世, 林克洋, 渡邊孝治, 松原秀憲, 土屋弘行
    • Organizer
      日本創外固定・骨延長学会
    • Place of Presentation
      東京
    • Year and Date
      2012-01-13
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット脂肪由来再生細胞移植による骨延長部骨形成促進法の開発2012

    • Author(s)
      野村一世, 渡邊孝治, 松原秀憲, 林克洋, 土屋弘行
    • Organizer
      第27回 日本整形外科学会基礎学術総会
    • Place of Presentation
      愛知県・名古屋市・名古屋国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] Adipose-derived regenerative cells promote bone formation on distraction osteogenesis in rats.2012

    • Author(s)
      Nomura I, Watanabe K, Matsubara H, Hayashi K, Tsuchiya H
    • Organizer
      7th International ASAMI and Bone Reconstruction Meeting
    • Place of Presentation
      Thessaloniki, Greece
    • Related Report
      2012 Research-status Report
  • [Presentation] 下肢長管骨感染性および非感染性偽関節に対するヨード担持チタンプレートによる治療2012

    • Author(s)
      渡邊孝治、松原秀憲、野村一世、土屋弘行
    • Organizer
      第85回日本整形外科学会学術総会
    • Place of Presentation
      京都府・京都市・京都国際会議場
    • Related Report
      2012 Research-status Report
  • [Presentation] A surgical procedure for fracture healing with superior soft tissue preservation2012

    • Author(s)
      Watanabe K, Matsubara H, Nomura I, Tsuchiya H.
    • Organizer
      7th International ASAMI and Bone Reconstruction Meeting
    • Place of Presentation
      Thessaloniki, Greece
    • Related Report
      2012 Research-status Report
  • [Presentation] Accuracy of tibial deformity correction with the Taylor Spatial Frame2012

    • Author(s)
      Watanabe K, Matsubara H, Nomura I, Tsuchiya H.
    • Organizer
      2nd International Confederation of EF and BR
    • Place of Presentation
      Salvador, Brasil
    • Related Report
      2012 Research-status Report
  • [Presentation] ラット脂肪由来間葉系細胞移植による骨延長部骨形成促進法の開発

    • Author(s)
      野村一世 林克洋 渡邊孝治 松原秀憲 土屋弘行
    • Organizer
      日本整形外科学会基礎学術集会
    • Place of Presentation
      千葉 幕張メッセ
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する

    • Author(s)
      野村一世 林克洋 渡邊孝治 松原秀憲 土屋弘行
    • Organizer
      第120回中部日本整形外科災害外科学会・学術集会
    • Place of Presentation
      和歌山 和歌山県民文化会館
    • Related Report
      2013 Annual Research Report
  • [Presentation] Autogenous adipose-derived regenerative cells promote bone formation on distraction osteogenesis in rats

    • Author(s)
      I. Nomura, K. Watanabe, H. Matsubara, K. Hayashi, H. Tsuchiya
    • Organizer
      wenty-third Annual Scientific Meeting.Limb Lengthening and Reconstruction Society: ASAMI-North America
    • Place of Presentation
      ニューヨーク Rosevelt Hotel
    • Related Report
      2013 Annual Research Report
  • [Presentation] ラット脂肪由来再生細胞移植は延長仮骨の成熟を促進する

    • Author(s)
      野村一世 林克洋 渡邊孝治 松原秀憲 土屋弘行
    • Organizer
      第32回日本運動器移植・再生医学研究会
    • Place of Presentation
      神戸 神戸大学 医学部会館シスメックスホール・神緑会館
    • Related Report
      2013 Annual Research Report
  • [Remarks] 骨再建・足の外科班

    • URL

      http://ortho.w3.kanazawa-u.ac.jp/intro/pages/foot_group.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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