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Elucidation of the role of mTOR in murine osteosarcoma cell line

Research Project

Project/Area Number 24791531
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionUniversity of Yamanashi

Principal Investigator

ANDO Takashi  山梨大学, 医学工学総合研究部, 助教 (10377492)

Research Collaborator HARO Hirotaka  山梨大学, 医学工学総合研究部, 教授 (10313264)
SATO Nobutaka  山梨大学, 医学工学総合研究部, 助教 (00418716)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨・軟部腫瘍学 / mTOR / 骨肉腫 / ゲムシタビン / mTOR / Gemcitabine / osteosarcoma / LM8 / lung metastasis
Research Abstract

mTOR inhibitor (Rapamycin) induced significant tumor growth inhibitory effect on mouse osteosarcoma cell line (LM8) by in vitro study as WB assay, WST assay, microscopic analysis and FACS analysis. Additive effect was observed by combining another anticancer agent (gemcitabine). mTOR inhibitor (rapamycin) induced the growth inhibition of primary tumor and suppression of lung metastasis in in vivo experiments using the osteosarcoma (LM8) tumor-bearing mouse model transplanted to C3H mice. Furthermore, same additive effect as in vitro study with the combination anti-cancer agent (gemcitabine) were observed in this mouse model study.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (12 results)

All 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Remarks (3 results)

  • [Journal Article] Bisphosphonates Inhibit Osteosarcoma-Mediated Osteolysis Via Attenuation of Tumor Expression of MCP-1 and RANKL2014

    • Author(s)
      Ohba T, Cole HA, Cates JM, Slosky DA, Haro H, Ando T, Schwartz HS, Schoenecker JG
    • Journal Title

      J Bone Miner Res

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pleiotropic effects of bisphosphonates on osteosarcoma2014

    • Author(s)
      Ohba T, Cates JM, Cole HA, Slosky DA, Haro H, Ichikawa J, Ando T, Schwartz HS, Schoenecker JG
    • Journal Title

      Bone

      Volume: 63 Pages: 110-120

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Autocrine VEGF/VEGFR1 Signaling in a Subpopulation of Cells Associates with Aggressive Osteosarcoma2014

    • Author(s)
      Ohba T, Cates JM, Cole HA, Slosky DA, Haro H, Ando T, Schwartz HS, Schoenecker JG
    • Journal Title

      Mol Cancer Res

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Endogenous TGF-beta activity limits TSLP expression in the intervertebral disc tissue by suppressing NF-kappaB activation2013

    • Author(s)
      Zhu Y, Ohba T, Ando T, Fujita K, Koyama K, Nakamura Y, Katoh R, Haro H, Nakao A
    • Journal Title

      Journal of orthopaedic research

      Volume: 31 Pages: 1144-1149

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 骨肉腫細胞株に対するmTOR 阻害剤(Rapamycin)とDNA 合成阻害剤(Gemcitabin)の併用による抗腫瘍効果についての検討2014

    • Author(s)
      安藤隆, 市川二郎, 佐藤信隆, 大北弦樹, 若生政憲, 波呂浩孝
    • Organizer
      第47回日本整形外科学会骨・軟部腫瘍学術集会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨肉腫細胞でのトロンビン産生能とトロンビンへの応答性2014

    • Author(s)
      市川二郎, Schoenecker Jonathan Schwartz Herbert, 安藤隆, 佐藤栄一, 波呂浩孝
    • Organizer
      第87回日本整形外科学会学術集会
    • Place of Presentation
      神戸国際会議場(神戸市)
    • Related Report
      2013 Final Research Report
  • [Presentation] Hylan G-F20 は軟骨細胞の分化を促進し, 変性を抑制する2014

    • Author(s)
      安藤隆, 佐藤栄一, 市川二郎, 若生政憲, 大北弦樹, 佐藤信隆, 落合聡司, 萩野哲男, 波呂浩孝
    • Organizer
      第27回日本軟骨代謝学会
    • Place of Presentation
      京都医師会館(京都市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 骨肉腫細胞株に対するmTOR阻害剤(Rapamycin)とDNA合成阻害剤(Gemcitabin)の併用による抗腫瘍効果についての検討2014

    • Author(s)
      安藤隆、市川二郎、佐藤信隆、齋藤正憲、大場哲郎、波呂浩孝
    • Organizer
      第47回日本整形外科学会 骨・軟部腫瘍学術集会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] マウス骨芽細胞へのThrombin刺激はMCP-1を発現させマクロファージの遊走を促進させる2013

    • Author(s)
      安藤隆, 市川二郎, 大場哲郎, 佐藤信隆, 大北弦樹, 波呂浩孝
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ(千葉市)
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.med.yamanashi.ac.jp/clinical/orthop/research.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 山梨大学医学部整形外科

    • URL

      http://www.med.yamanashi.ac.jp/clinical/orthop/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 山梨大学医学部整形外科(研究)

    • URL

      http://www.med.yamanashi.ac.jp/clinical/orthop/research.html

    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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