• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of culture system for tenocyte and identification of novel micro RNA in tenocyte differentiation

Research Project

Project/Area Number 24791532
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Orthopaedic surgery
Research InstitutionShinshu University

Principal Investigator

ITSUBO Toshiro  信州大学, 医学部附属病院, 特任研究員 (90467168)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords腱細胞 / GDF8 / 筋芽細胞株 / マイクロRNA / 標的遺伝子 / Smad7 / miRNA / Smad / Tppp3 / 無血清培地
Outline of Final Research Achievements

Recently, microRNAs(miRNAs) have been emerged as important regulators in cell differentiation, but miRNA which regulates tenocyte differentiation has not been identified. We found that GDF8 promoted differentiation of mouse myoblast cell line into tenocyte through Smad2/3 signaling. Microarray assay showed that expression of eight miRNAs, including miR-214, increased after induction of tenocyte differentiation and Smad7 was identified as miR-214 target gene. Since Smad7 is negative regulator of Smad2/3 signaling, our data suggest that miR-214 promotes tenocyte differentiation.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (3 results)

All 2014 2013

All Presentation (3 results)

  • [Presentation] マウス筋芽細胞株を用いた腱細胞培養系の確立2014

    • Author(s)
      植村一貴,林正徳,内山茂晴,伊坪敏郎,加藤博之
    • Organizer
      第57回日本手外科学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県・宜野湾市)
    • Year and Date
      2014-04-17 – 2014-04-18
    • Related Report
      2014 Annual Research Report
  • [Presentation] GDF-8 induces differentiation of myoblastic C2C12 cells into tenocytes2014

    • Author(s)
      植村一貴,林正徳,内山茂晴,伊坪敏郎,加藤博之
    • Organizer
      Orthopaedic Research Society 2014 Annual meeting
    • Place of Presentation
      Hyatt Regency New Orleans
    • Related Report
      2013 Research-status Report
  • [Presentation] マウス筋芽細胞株を用いた腱細胞培養系の確立2013

    • Author(s)
      植村一貴,林正徳,内山茂晴,伊坪敏郎,加藤博之
    • Organizer
      第28回日本整形外科学会基礎学術集会
    • Place of Presentation
      幕張メッセ国際会議場
    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi