Establishment of culture system for tenocyte and identification of novel micro RNA in tenocyte differentiation

Research Project

Project/Area Number	24791532
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Orthopaedic surgery
Research Institution	Shinshu University
Principal Investigator	ITSUBO Toshiro 信州大学, 医学部附属病院, 特任研究員 (90467168)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	腱細胞 / GDF8 / 筋芽細胞株 / マイクロRNA / 標的遺伝子 / Smad7 / miRNA / Smad / Tppp3 / 無血清培地
Outline of Final Research Achievements	Recently, microRNAs(miRNAs) have been emerged as important regulators in cell differentiation, but miRNA which regulates tenocyte differentiation has not been identified. We found that GDF8 promoted differentiation of mouse myoblast cell line into tenocyte through Smad2/3 signaling. Microarray assay showed that expression of eight miRNAs, including miR-214, increased after induction of tenocyte differentiation and Smad7 was identified as miR-214 target gene. Since Smad7 is negative regulator of Smad2/3 signaling, our data suggest that miR-214 promotes tenocyte differentiation.

Report

(4 results)