| Project/Area Number |
24791541
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
OKADA Minoru 京都大学, iPS細胞研究所, 研究員 (30625835)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TSUMAKI Noriyuki 京都大学, iPS細胞研究所, 教授
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 整形外科学 / 細胞生物学 / 分子生物学 / 骨系統疾患 / 軟骨 / 疾患モデル / 小胞体ストレス / アポトーシス / 小胞体 |
|---|
| Research Abstract |
The purpose is to clarify mechanisms of type II collagenopathy and to find drugs for medical application. In this study, we established disease models of type II collagenopathy using cell reprogramming technologies. To generate disease-specific chondrocytes, we introduced retrovirus or plasmid vectors encoding specific genes to patients fibroblasts. Disease-specific chondrocytes derived from patients fibroblasts or iPS cells showed intracellular accumulation of mutated type II collagen and endoplasmic reticulum stress, resulting apoptosis. These disease models reflect phenotypes of type II collagenopathy and can be useful for drug screening in further investigations.
|
