Mechanisms of intervertebral disc degeneration in postmenopausal women

• Project/Area Number: 24791562
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: National Defense Medical College (2013-2015); Keio University (2012)
• Principal Investigator: Hosogane Naobumi 防衛医科大学校(医学教育部医学科進学課程及び専門課程、動物実験施設、共同利用研究, その他部局等, 助教 (10365306)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 椎間板 / 退行変性 / 活性酸素 / 椎間板変性 / 酸化ストレス

Outline of Final Research Achievements

Intervertebral disc (IVD) degeneration was accelerated in ovariectomy rats, although the phenotype was not obvious enough to perform further analysis. Therefore, IVD degeneration was induce by disc puncture with needle and revealed that oxidative stress was involved in the degeneration. mRNA expression of catabolic factors such as TNF-alpha, MMP-3, COX-2 were increased in degenerated IVD of punctured rats. In vitro study, oxidative stress induced with H2O2 upregulated the expressions of these catabolic factors. Finally, degeneration of IVD induced with oxidative stress was rescued with administration of antioxidant NAC (N-acteyl cysteine) both in vivo and in vitro analysis.

Research Products

• [Journal Article] Excessive reactive oxygen species are therapeutic targets for intervertebral disc degeneration. (2015)
  • Author(s): Suzuki S, Fujita N, Hosogane N, Watanabe K, Ishii K, Toyama Y, Takubo K, Horiuchi K, Miyamoto T, Nakamura M, Matsumoto M.
  • Journal Title: Arthritis Res Ther. Volume: 17 Issue: 1 Pages: 316333-316333
  • DOI: 10.1186/s13075-015-0834-8
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Serum cartilage metabolites as biomarkers of degenerative lumbar scoliosis (2012)
  • Author(s): Hosogane N, et al
  • Journal Title: J Orthop Res Volume: (In press) Issue: 8 Pages: 1249-53
  • DOI: 10.1002/jor.22067
  • Peer Reviewed
• [Presentation] 椎間板変性に対する抗酸化剤の効果 慢性腰痛患者の減少を目指して (2016)
  • Author(s): 藤田 順之, 鈴木 悟士, 日方 智宏, 岩波 明生, 渡辺 航太, 細金 直文, 辻 崇, 石井 賢, 中村 雅也, 松本 守雄
  • Organizer: 日本脊椎脊髄病学会
  • Place of Presentation: 幕張
  • Year and Date: 2016-04-14
• [Presentation] 活性酸素種は椎間板変性に対する治療標的となりうるか (2015)
  • Author(s): 鈴木 悟士, 藤田 順之, 細金 直文, 渡邊 隆一, 日方 智宏, 岩波 明生, 渡邉 航太, 石井 賢, 戸山 芳昭, 田久保 圭誉, 堀内 圭輔, 宮本 健史, 中村 雅也, 松本 守雄
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 富山
  • Year and Date: 2015-10-22
• [Presentation] Reactive oxygen species are therapeutic targets for intervertebral disc degeneration (2015)
  • Author(s): Satoshi Suzuki, Nobuyuki Fujita, Naobumi Hosogane, Ryuichi Watanabe, Tomohiro Hikata, Akio Iwanami, Kota Watanabe, Ken Ishii, Yoshiaki Toyama, Keiyo Takubo, Keisuke Horiuchi, Takeshi Miyamoto, Masaya Nakamura, Morio Matsumoto
  • Organizer: Orthopedic Research Society
  • Place of Presentation: 米国ラスベガス
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] 活性酸素種は椎間板変性の治療標的である (2015)
  • Author(s): 鈴木悟士 藤田順之 細金直文 石井賢 渡邊隆一 日方智宏 渡辺航太 堀内圭輔 戸山芳昭 宮本健史 松本守雄
  • Organizer: 日本軟骨代謝学会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-06 – 2015-03-07
• [Presentation] 椎間板変性に対する抗酸化剤の効果 (2014)
  • Author(s): 鈴木悟士 藤田順之 細金直文 石井賢 渡邊隆一 日方智宏 田久保圭誉 渡辺航太 戸山芳昭 宮本健史 松本守雄
  • Organizer: 日本腰痛学会
  • Place of Presentation: 千葉
  • Year and Date: 2014-11-15 – 2014-11-16
• [Presentation] 椎間板変性と酸化ストレスの関係 (2014)
  • Author(s): 鈴木悟士 藤田順之 細金直文 石井賢 渡邊隆一 日方智宏 田久保圭誉 渡辺航太 戸山芳昭 宮本健史 松本守雄
  • Organizer: 日本整形外科学会基礎学術集会
  • Place of Presentation: 鹿児島
  • Year and Date: 2014-10-09 – 2014-10-11
• [Presentation] Molecular Characterization of Chordoma Cell Line U-CH1-N: Applications for Intervertebral Disc Research (2014)
  • Author(s): Nobuyuki Fujita, Naobumi Hosogane, Ken Ishii, Tomohiro Hikata, Yoshiaki Toyama, Morio Matsumoto,, Takeshi Miyamoto
  • Organizer: Orthopedic Research Society
  • Place of Presentation: 米国、New Orleans
• [Presentation] Cartilage biomakers in degenerative lumbar scoliosis. (2012)
  • Author(s): Hosogane N, Iwanami A, Watanabe K, Tsuji T, Miyamoto T, Ishii K, Niki Y, Nakamura M, Toyama Y, Chiba K, Matsumoto M.
  • Organizer: International Society for the Study of the Lumbar Spine
  • Place of Presentation: オランダ