| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Intervertebral disc (IVD) degeneration was accelerated in ovariectomy rats, although the phenotype was not obvious enough to perform further analysis. Therefore, IVD degeneration was induce by disc puncture with needle and revealed that oxidative stress was involved in the degeneration. mRNA expression of catabolic factors such as TNF-alpha, MMP-3, COX-2 were increased in degenerated IVD of punctured rats. In vitro study, oxidative stress induced with H2O2 upregulated the expressions of these catabolic factors. Finally, degeneration of IVD induced with oxidative stress was rescued with administration of antioxidant NAC (N-acteyl cysteine) both in vivo and in vitro analysis.
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