| Project/Area Number |
24791616
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SAWADA Maiko 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90330860)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | micro RNA / 敗血症 / 単球 / 貪食能 / マクロファージ / 遺伝子治療 |
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| Research Abstract |
Increased cell death and decline in the phagocytic activity of the monocytic cell line markedly reduce the dead cell clearance rate, thereby prolonging the inflammatory response. We previously reported that stress-induced hyperglycemia during sepsis led to malfunctioning of the monocytic cell line and aggravated the inflammatory response.
Recent advances have shown that microRNA expression plays important roles in the phagocytic activity of macrophages. Based on these findings, we analyzed the pattern of microRNA expression during sepsis. Our results revealed that microRNA-21 played an important role in the phagocytic activity of macrophages under the studied conditions. This finding led us to the conclusion that in addition to microRNA-21, other microRNAs could also be associated with the phagocytic activity of macrophages. Presently, we are conducting comprehensive analyses using next-generation sequencing to identify more microRNAs associated with macrophage phagocytic activity.
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