Changes in microRNA expression pattern and their effects on sepsis
Project/Area Number |
24791616
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Anesthesiology/Resuscitation studies
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
SAWADA Maiko 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90330860)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
|
Keywords | micro RNA / 敗血症 / 単球 / 貪食能 / マクロファージ / 遺伝子治療 |
Research Abstract |
Increased cell death and decline in the phagocytic activity of the monocytic cell line markedly reduce the dead cell clearance rate, thereby prolonging the inflammatory response. We previously reported that stress-induced hyperglycemia during sepsis led to malfunctioning of the monocytic cell line and aggravated the inflammatory response. Recent advances have shown that microRNA expression plays important roles in the phagocytic activity of macrophages. Based on these findings, we analyzed the pattern of microRNA expression during sepsis. Our results revealed that microRNA-21 played an important role in the phagocytic activity of macrophages under the studied conditions. This finding led us to the conclusion that in addition to microRNA-21, other microRNAs could also be associated with the phagocytic activity of macrophages. Presently, we are conducting comprehensive analyses using next-generation sequencing to identify more microRNAs associated with macrophage phagocytic activity.
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Report
(3 results)
Research Products
(12 results)
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[Presentation] ケアへの取り組み2013
Author(s)
澤田麻衣子, 松山広樹, 野口綾子, 橋本壮志, 徳平夏子, 濱岡早枝子, 黄瀬ひろみ, 木村彰夫橋本悟, 佐和貞治
Organizer
第40回日本集中治療医学会当院における急性期患者における終末期医療
Place of Presentation
松本
Year and Date
2013-03-01
Related Report
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