Mechanisms of lipid infusion on local anesthetic-induced cardiac toxicity

• Project/Area Number: 24791619
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka City University
• Principal Investigator: MATSUURA Tadashi 大阪市立大学, 医学(系)研究科(研究院), 講師 (90619793)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 局所麻酔薬中毒 / 脂肪乳剤 / パッチクランプ / ランゲンドルフ / 局所麻酔薬 / 単離心筋細胞

Outline of Final Research Achievements

Lipid emulsion has been reported to be quite effective against local anesthetic-induced systemic toxicity. This study aimed to investigate the mechanisms of lipid infusion therapy. Proton channels recordings by patch-clamp method revealed that extracellular lipid emulsion trapped bupivacaine to decrease the concentration of extracellular bupivacaine and consequently the intracellular concentration of bupivacaine was decreased. This result supports the importance of "lipid sink" of lipid infusion therapy. Both results of lagendorff experiments and in vivo experiments could also suggest that "lipid sink" is the main mechanism of lipid infusion therapy.

Research Products

• [Journal Article] Detection of left ventricular dysfunction using early diastolic mitral annular velocity in patients undergoing mitral valve repair for mitral regurgitation (2014)
  • Author(s): Koichi Suehiro, Katsuaki Tanaka, Tadashi Matsuura, Tomoharu Funao, Tokuhiro Yamada, Takashi Mori, Kiyonobu Nishikawa
  • Journal Title: Journal of Cardiothoracic and Vascular Anesthesia Volume: 28 Issue: 1 Pages: 25-30
  • DOI: 10.1053/j.jvca.2013.07.013
  • Peer Reviewed
• [Journal Article] The effect of lipid emulsion on intracellular bupivacaine as a mechanism of lipid resuscitation: an electrophysiological study using voltage-gated proton channels. (2013)
  • Author(s): Kotaro Hori, Tadashi Matsuura, Takashi Mori, Miyuki Kuno, Makoto Sawada, Kiyonobu Nishikawa
  • Journal Title: Anesthesia & Analgesia Volume: 117 Issue: 6 Pages: 1293-1301
  • DOI: 10.1213/ane.0000000000000011
  • Peer Reviewed
• [Journal Article] Systemic vascular resistance has an impact on the reliability of the Vigileo-FloTrac system in measuring cardiac output and tracking cardiac output changes (2013)
  • Author(s): Koichi Suehiro
  • Journal Title: British Journal of Anaesthesia Volume: 110 Issue: 2 Pages: 1-8
  • DOI: 10.1093/bja/aet022
  • Peer Reviewed
• [Journal Article] Effects of general anesthetics on P2X_4 receptors in a mouse microglial cell line (2012)
  • Author(s): Megumi Hasaka, Takashi Mori, Tadashi Matsuura, Toshio Narahashi, Miyuki Kuno, Akira Asada, Kiyonobu Nishikawa
  • Journal Title: NeuroReport Volume: (in press) Issue: 10 Pages: 601-605
  • DOI: 10.1097/wnr.0b013e32835509db
  • Peer Reviewed
• [Presentation] The electrophysiological effects of lipid emulsion on bupivacaine-induced cardiotoxicity in guinea pigs (2014)
  • Author(s): Kotaro Hori, Tadashi Matsuura, Shogo Tsujikawa, Megumi Hasaka, Takashi Mori, Miyuki Kuno, Kiyonobu Nishikawa
  • Organizer: American Society of Anesthesiologist annual meeting 2014
  • Place of Presentation: New Orleans, Louisiana (USA)
  • Year and Date: 2014-10-11 – 2014-10-15
• [Presentation] リピッドレスキューによる血行動態回復におけるlipid sink effectの重要性 (2014)
  • Author(s): 堀耕太郎、松浦 正、辻川翔吾、森 隆、久野みゆき、西川精宣
  • Organizer: 日本麻酔科学会第61回学術集会
  • Place of Presentation: パシフィコ横浜（神奈川県 横浜市）
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] The recovery speed of lipid emulsion therapy largely depends on “lipid sink”: an electrophysiological study using voltage-gated proton channels (2013)
  • Author(s): 2.Kotaro Hori, Tadashi Matsuura, Megumi Hasaka, Takashi Mori, Miyuki Kuno, Kiyonobu Nishikawa
  • Organizer: Euroanaesthesia 2013
  • Place of Presentation: Fotos y Consideraciones, Barcelona, Spain
• [Presentation] Prediction of left ventricular dysfunction using early diastolic mitral annular velocity after MVR for severe MR
  • Author(s): 3.Koichi Suehiro, Katsuaki Tanaka, Tadashi Matsuura, Tomoharu Funao, Tokuhiro Yamada, Takashi Mori, Kiyonobu Nishikawa
  • Organizer: 2013 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Moscone Convention Center, San Francisco, California, USA
• [Presentation] Discrepancy between ScvO2 and SvO2 is a reliable index to predict postoperative complications in cardiac surgery
  • Author(s): 4.Koichi Suehiro, Katsuaki Tanaka, Tadashi Matsuura, Tomoharu Funao, Tokuhiro Yamada, Takashi Mori, Kiyonobu Nishikawa
  • Organizer: 2013 Annual Meeting, American Society of Anesthesiologists
  • Place of Presentation: Moscone Convention Center, San Francisco, California, USA
• [Presentation] lipid rescueにおける“lipid sink”の効果発現時間について ~電位依存性プロトンチャネルによる検討
  • Author(s): 1.堀耕太郎, 松浦正, 羽阪めぐみ, 森隆, 久野みゆき, 西川精宣
  • Organizer: 日本麻酔科学会第60回学術集会
  • Place of Presentation: 札幌市教育文化会館、札幌
• [Presentation] 電位依存性プロトンチャネルによるlipid rescueメカニズムの検討
  • Author(s): 堀耕太郎、松浦 正、羽阪めぐみ、森 隆、久野みゆき、西川精宣
  • Organizer: 日本麻酔科学会 第59回学術集会
  • Place of Presentation: 神戸ポートピアホテル