Tumor-suppressive microRNA-145 regulates molecular pathways based on microRNA expression signature in prostate cancer

• Project/Area Number: 24791636
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: Dokkyo Medical University
• Principal Investigator: FUSE Miki 獨協医科大学, 医学部, 助教 (90568627)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: マイクロRNA / 前立腺癌 / miR-143 / miR-145 / 癌抑制遺伝子 / 癌遺伝子

Research Abstract

Our recent study of microRNA (miRNA) expression signature of prostate cancer (PCa) has revealed that the microRNA-143/145 (miR-143/145) cluster is significantly downregulated in cancer tissues. Restoration of miR-143 or miR-145 in PCa cell lines showed that these miRNAs significantly inhibited cancer cell migration and invasion, this findings suggesting that these clustered miRNAs function as tumor suppressors. Gene expression studies and luciferase reporter assays showed that Golgi membrane 1 (GOLM1) was directly regulated by the miR-143/145 cluster. Silencing of GOLM1 resulted in significant inhibition of cell migration and invasion in PCa cells. Furthermore, the expression of GOLM1 was upregulated in cancer tissues by immunohistochemistry.

Research Products

• [Journal Article] 1. Tumor-suppressive microRNA-29s inhibit cancer cell migration and invasion via (2014)
  • Author(s): Nishikawa R, Goto Y, Kojima S, Enokida H, Chiyomaru T, Kinoshita T, Sakamoto S, Fuse M, Nakagawa M, Naya Y, Ichikawa T, Seki N
  • Journal Title: Int J Oncol. Volume: 45(1) Issue: 1 Pages: 401-410
  • DOI: 10.3892/ijo.2014.2437
  • Peer Reviewed
• [Journal Article] Tumour-suppressive microRNA-224 inhibits cancer cell migration and invasion via targeting oncogenic TPD52 in prostate cancer. (2014)
  • Author(s): Goto Y, Nishikawa R, Kojima S, Chiyomaru T, Enokida H, Inoguchi S, Kinoshita T, Fuse M, Sakamoto S, Nakagawa M, Naya Y, Ichikawa T, Seki N.
  • Journal Title: FEBS Lett. Volume: 588(10) Issue: 10 Pages: 1973-1982
  • DOI: 10.1016/j.febslet.2014.04.020
  • Peer Reviewed
• [Journal Article] The tumor-suppressive microRNA-143/145 cluster inhibits cell migration and invasion by targeting GOLM1 in prostate cancer. (2014)
  • Author(s): Kojima S, Enokida H, Yoshino H, Itesako T, Chiyomaru T, Kinoshita T, Fuse M, Nishikawa R, Goto Y, Naya Y, Nakagawa M, Seki N.
  • Journal Title: J Hum Genet. Volume: 59(2) Issue: 2 Pages: 78-87
  • DOI: 10.1038/jhg.2013.121
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal transition-related microRNA-200s regulate molecular targets and pathways in renal cell carcinoma. (2013)
  • Author(s): Hirofumi Yoshino, Hideki Enokida, Toshihiko Itesako, Shuichi Tatarano, Takashi Kinoshita, Miki Fuse, Satoko Kojima, Masayuki Nakagawa and Naohiko Seki.
  • Journal Title: J Hum Genet. Volume: 58(8) Issue: 8 Pages: 508-516
  • DOI: 10.1038/jhg.2013.31
  • NAID: 10031195279
  • Peer Reviewed
• [Journal Article] Tumor suppressive microRNAs (miR-222 and miR-31) regulate molecular pathways based on microRNA expression signature in prostate cancer. (2012)
  • Author(s): 1) Miki Fuse, Satoko Kojima,Enokida H, Chiyomaru T, Yoshino H, Niziro Nohata, Takashi Kinoshita, Shinich Sakamoto, Yukio Naya, Masayuki Nakagawa, Tomohiko Ichikawa, Naohiko Seki.
  • Journal Title: J Hum Genet. Volume: 57(11) Issue: 11 Pages: 691-699
  • DOI: 10.1038/jhg.2012.95
  • NAID: 10031126990
  • Peer Reviewed
• [Journal Article] Novel molecular targets regulated by tumor suppressors microRNA-1 and microRNA-133a in bladder cancer (2012)
  • Author(s): Yamasaki, at al
  • Journal Title: International Journal of Oncology Volume: 40 Pages: 1821-1830
  • DOI: 10.3892/ijo.2012.1391
  • Peer Reviewed
• [Presentation] Molecular targets regulated by tumor suppressive microRNA-1 and microRNA-133a in prostate cancer (2012)
  • Author(s): Miki Fuse, Satoko Kojima, Takeshi Chiyomaru, Hirofumi Yoshino, Nijiro Nohata, Masayuki Nakagawa, Tomohiko Ichikawa, Naohiko Seki
  • Organizer: AACR 103rd Annual Meeting
  • Place of Presentation: Chicago, IL, USA
• [Remarks]
  • URL: http://genomejet.jp