| Project/Area Number |
24791636
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Dokkyo Medical University |
|---|
Principal Investigator |
FUSE Miki 獨協医科大学, 医学部, 助教 (90568627)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | マイクロRNA / 前立腺癌 / miR-143 / miR-145 / 癌抑制遺伝子 / 癌遺伝子 |
|---|
| Research Abstract |
Our recent study of microRNA (miRNA) expression signature of prostate cancer (PCa) has revealed that the microRNA-143/145 (miR-143/145) cluster is significantly downregulated in cancer tissues. Restoration of miR-143 or miR-145 in PCa cell lines showed that these miRNAs significantly inhibited cancer cell migration and invasion, this findings suggesting that these clustered miRNAs function as tumor suppressors. Gene expression studies and luciferase reporter assays showed that Golgi membrane 1 (GOLM1) was directly regulated by the miR-143/145 cluster. Silencing of GOLM1 resulted in significant inhibition of cell migration and invasion in PCa cells. Furthermore, the expression of GOLM1 was upregulated in cancer tissues by immunohistochemistry.
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