Research on mechanism of the ATP release from urinary bladder epithelium of disease models upon mechanical strech stimulation

• Project/Area Number: 24791641
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Urology
• Research Institution: University of Yamanashi
• Principal Investigator: KIRA Satoru 山梨大学, 医学部附属病院, 医員 (10530115)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 排尿障害 / VNUT / 膀胱 / 知覚神経伝達 / 過活動膀胱 / 膀胱上皮

Research Abstract

In this study, we have been trying to research on mechanism of VNUT in urinary bladder epithelium in various conditions. Knock down of VNUT in bladder epithelium culture cells decreased ATP release upon mechanical stretch stimulation. Furthermore, we investigated VNUT in human urinary bladder. mRNA and protein of VNUT were highly expressed in the human urinary bladder epithelium. The mRNA of VNUT level was demonstrated to be negatively correlated with First desire to void. According to these results, control of VNUT may provide a novel strategy for the treatment of bladder dysfunction.

Research Products

• [Journal Article] Tailor-Made Mesh for Pelvic Organ Prolapses : Correlation between Patient's Height and Mesh Size (2013)
  • Author(s): Hideki Kobayashi, Yaburu Haneda, Satoru Kira, Takayuki Tsuchida, Isao Araki, Masayuki Takeda
  • Journal Title: Open Journal of Urology Volume: 3 Pages: 121-123
  • Peer Reviewed
• [Journal Article] TVM メッシュの大きさに関する検討 (2013)
  • Author(s): 小林 英樹, 羽根田 破, 吉良 聡, 宮本 達也, 澤田 智史, 土田 孝之, 荒木 勇雄, 武田 正之
  • Journal Title: 日本女性骨盤底医学会誌 Volume: 10(1) Pages: 216-218
  • Peer Reviewed
• [Journal Article] Tailor-Made Mesh for Pelvic Organ Prolapses: Correlation between Patient's Height and Mesh Size (2013)
  • Author(s): Hideki Kobayashi Yaburu Haneda Satoru Kira Takayuki Tsuchida Isao Araki Masayuki Takeda
  • Journal Title: Open Journal of Urology Volume: 3 Pages: 121-123
  • Peer Reviewed
• [Journal Article] Isao Araki, Masayuki Takeda Intraoperative Fluoroscopic Monitoring during TVM Surgery : Safer Procedure Even for Beginners (2012)
  • Author(s): Hideki Kobayashi, Norifumi SAWADA, Satoru Kira, Tatsuya Miyamoto, Yaburu Haneda, Hidenori Zakoji, Takayuki Tsuchida
  • Journal Title: Open Journal of Urology Volume: 2(2) Pages: 72-74
  • Peer Reviewed
• [Journal Article] TOT 手術における術前後の排尿状態の変化に関する検討 (2012)
  • Author(s): 吉良 聡, 羽根田 破, 小林 英樹, 荒木 勇雄, 武田 正之
  • Journal Title: 日本女性骨盤底医学会誌 Volume: 9(1) Pages: 118-121
  • Peer Reviewed
• [Journal Article] 透視下 TVM 手術の検討 (2012)
  • Author(s): 小林 英樹, 澤田 智史, 今井 佑樹, 吉良 聡, 宮本 達也, 羽根田 破, 荒木 勇雄, 武田 正之
  • Journal Title: 日本女性骨盤底医学会誌 Volume: 9(1) Pages: 68-71
  • Peer Reviewed
• [Journal Article] 孤立性副腎転移に対する腹腔鏡下副腎摘除術の経験 (2012)
  • Author(s): 座光寺 秀典, 工藤 祥司, 犬塚 秀康, 吉良 聡, 宮本 達也, 武田 正之
  • Journal Title: 日本泌尿器科学会雑誌 Volume: 103(3) Pages: 535-539
  • NAID: 10030431398
  • Peer Reviewed
• [Journal Article] TOT手術における術前後の排尿状態の変化に関する検討 (2012)
  • Author(s): 吉良 聡、羽根田破、小林英樹、武田正之、荒木勇雄
  • Journal Title: 日本女性骨盤底医学会誌 Volume: 9 Pages: 118-121
  • Peer Reviewed
• [Presentation] Piezo1, a novel mechanosensor in the bladder urothelium, transmits signals of bladder secsation (2013)
  • Author(s): Tatsuya Miyamoto, Hiroshi Nakagomi, Satoru Kira, Tsutomu Mochizuki, Schuichi KOIZUMI, Makoto Tominaga, Masayuki Takeda
  • Organizer: 28th Annual EAU Congress
  • Place of Presentation: Milan, Italy
  • Year and Date: 2013-03-16
• [Presentation] ヒト膀胱上皮におけるVNUT ｍ－RNA発現量と各種パラメータとの関連性 (2013)
  • Author(s): 中込 宙史、望月 勉、川原 和也、宮本 達也、吉良 聡、荒木 勇雄、森山 芳則、小泉 修一、武田 正之
  • Organizer: 第101回日本泌尿器科学会総会
  • Place of Presentation: さっぽろ芸術文化の館（北海道札幌市）
• [Presentation] 膀胱上皮における新規メカノセンサー“Piezo1"の解明 (2013)
  • Author(s): 宮本 達也、中込 宙史、吉良 聡、望月 勉、小泉 修一、富永 真琴、武田 正之
  • Organizer: 第101回日本泌尿器科学会総会
  • Place of Presentation: さっぽろ芸術文化の館（北海道札幌市）
• [Presentation] 膀胱上皮における伸展刺激センサーPiezo1とTRPV4の関係性 (2013)
  • Author(s): 宮本 達也、中込 宙史、吉良 聡、望月 勉、小林 英樹、座光寺 秀典、土田 孝之、小泉 修一、富永 真琴、武田 正之
  • Organizer: 第20回日本排尿機能学会
  • Place of Presentation: グランシップ（静岡県静岡市）
• [Presentation] PIEZO1, A NOVEL MECHANOSENSOR IN THE BLADDER UROTHELIUM (2012)
  • Author(s): Tatsuya Miyamoto, Hiroshi Nakagomi, Satoru Kira, Tsutomu Mochizuki, Schuichi KOIZUMI, Makoto Tominaga, Masayuki Takeda
  • Organizer: 42nd Annual Meeting of the International Continence Society(ICS)
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-10-19
• [Presentation] VESICULAR NUCLEOTIDE TRANSPORTER(VNUT) IS A KEY MOLECULE FOR MECHANOSENSING IN THE HUMAN URINARY BLADDER : NEGATIVE CORRELATION BETWEEN VNUT EXPRESSION IN HUMAN BLADDER MUCOSA AND FIRST DESIRE TO VOID (2012)
  • Author(s): Hiroshi Nakagomi, Tsutomu Mochizuki, Tatsuya Miyamoto, Satoru Kira, Mitsuharu Yoshiyama, Kazuya Kawahara, Y Moriyama, Schuichi KOIZUMI, Masayuki Takeda
  • Organizer: 42nd Annual Meeting of the International Continence Society(ICS)
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-10-17
• [Presentation] TAILOR-MADE MESH FOR PELVIC ORGAN PROLAPSED PATIENTS IS BETTER THAN READY-MADE UNIFORM MESH (2012)
  • Author(s): Hideki Kobayashi, Yaburu Haneda, Satoru Kira, Tatsuya Miyamoto, Norifumi SAWADA, Takayuki Tsuchida, Isao Araki, Masayuki Takeda
  • Organizer: 42nd Annual Meeting of the International Continence Society(ICS)
  • Place of Presentation: Beijing, China
  • Year and Date: 2012-10-17
• [Presentation] Tailor-made mesh for pelvic organ prolapsed patients is better than ready-made uniform mesh (2012)
  • Author(s): Hideki Kobayashi, Satoru Kira, Yaburu Haneda, Tatsuya Miyamoto, Norifumi SAWADA, Takayuki Tsuchida, Isao Araki, Masayuki Takeda
  • Organizer: The 7th Pan-Pacific Continence Society Meeting
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2012-08-28
• [Presentation] PDGF-evoked Beta-catenin signaling contributes to bladder smooth muscle cell contraction through up-regulation of Cx43 expression (2012)
  • Author(s): Hiroshi Nakagomi, LI Kai, Norifumi SAWADA, Tatsuya Miyamoto, Satoru Kira, Masanori KITAMURA, Masayuki Takeda
  • Organizer: The 7th Pan-Pacific Continence Society Meeting
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2012-08-28
• [Presentation] Significant correlation between Vitamine D receptor and epithelial Ca2+ ion channel TRPV5 and TRPV6 in renal cell carcinoma (2012)
  • Author(s): WU Yongyang, Tatsuya Miyamoto, LI Kai, Hiroshi Nakagomi, Norifumi SAWADA, Satoru Kira, Hideki Kobayashi, Hidenori Zakoji, Isao Araki, Masayuki Takeda
  • Organizer: The 107th Annual Meeting of The American Urological Association
  • Place of Presentation: Atlanta, USA
  • Year and Date: 2012-05-20
• [Presentation] 腎細胞癌における上皮型カルシウムチャネルTRPV5/TRPV6、VitaminD受容体の発現低下 (2012)
  • Author(s): 吉良 聡
  • Organizer: 第100回日本泌尿器科学会
  • Place of Presentation: パシフィコ横浜（神奈川）
• [Remarks]
  • URL: http://www.uro-yamanashi.com/