Basic research for voiding dysfunction using iPS cells

Research Project

Project/Area Number	24791649
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Urology
Research Institution	Okayama University
Principal Investigator	SASAKI KATSUMI 岡山大学, 大学病院, 助教 (80467745)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords	iPS細胞 / 平滑筋細胞 / SGEプラスミド / 免疫染色 / 再生医療 / 膀胱平滑筋細胞
Outline of Final Research Achievements	Human or mouse iPS cells were cultured with retinoic acid and induced to smooth muscle cells. Immunohistchemical staining was performed to confirm the smooth muscle differentiation. Besides, preliminary experiments to establish efficient induction systems from iPS cells to the smooth muscle cells were performed. GFP (maker gene) was integrated to the plural SGE-plasmid system (Okayama Univ. original license). The hyper-GFP gene expression systems to iPS cells were established using these SEG-GFP-plasmid systems.

Report

(4 results)