Basic research for voiding dysfunction using iPS cells
| Project/Area Number |
24791649
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | iPS細胞 / 平滑筋細胞 / SGEプラスミド / 免疫染色 / 再生医療 / 膀胱平滑筋細胞 |
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| Outline of Final Research Achievements |
Human or mouse iPS cells were cultured with retinoic acid and induced to smooth muscle cells. Immunohistchemical staining was performed to confirm the smooth muscle differentiation. Besides, preliminary experiments to establish efficient induction systems from iPS cells to the smooth muscle cells were performed. GFP (maker gene) was integrated to the plural SGE-plasmid system (Okayama Univ. original license). The hyper-GFP gene expression systems to iPS cells were established using these SEG-GFP-plasmid systems.
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Report
(4 results)
Research Products
(1 results)
