Elucidation of epigenetic transcriptional regulation in germ cell differentiation
Project/Area Number |
24791662
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Urology
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Research Institution | Nagoya City University |
Principal Investigator |
NISHIO Hidenori 名古屋市立大学, 医学(系)研究科(研究院), 研究員 (10621063)
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Research Collaborator |
KOHRI Kenjiro
HAYASHI Yutaro
KOJIMA Yoshiyuki
MIZUNO Kentaro
KUROKAWA Satoshi
KAMISAWA Hideyuki
MORITOKI Yoshinobu
|
Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | 精子幹細胞 / ヒストン修飾 / 脱メチル化 / エピジェネティクス |
Outline of Final Research Achievements |
In present study, the aim is to elucidate the mechanism underlying gonocyte transformation to spermatogonial stem cells during spermatogenesis. Kdm5a expression was significantly higher at 9 days postpartum in the undescended testes of experimentally induced cryptorchid model rats than in the normal testes. Since Kdm5a regulates histone H3K4me2/me3 demethylation, H3K4me3/me2 expression levels were decreased in the undescended testes. Moreover, the expression of genes related to spermatogonial stem cell development was induced in spermatogonial cells over expressing Kdm5a. Thus, Kdm5a is likely involved in the transformation of gonocytes into spermatogonial stem cells by transcriptional regulation of specific genes via H3K4 histone modification. This is the first report of epigenetic analysis of germ cell differentiation during early spermatogenesis.
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Report
(4 results)
Research Products
(9 results)
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[Presentation] Distinctive changes in histone H3K4 modification mediated via Kdm5a expression in spermatogonial stem cells of cryptorchid testes2014
Author(s)
Nishio Hidenori, Hayashi Yutaro, Moritoki Yoshinobu, Kamisawa Hideyuki, Kurokawa Satoshi, Nakane Akihiro, Mizuno Kentaro, Maruyama Tetsuji, Kojima Yoshiyuki, Kohri Kenjiro
Organizer
American Urological Association Annual Meeting 2014
Place of Presentation
Orange County Convention Center (Orlando,USA)
Year and Date
20140516-20140521
Related Report
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[Presentation] Distinctive changes in histone H3K4 modification mediated via Kdm5a expression in spermatogonial stem cells of cryptorchid testes2014
Author(s)
Hidenori Nishio, Yutaro Hayashi, Yoshinobu Moritoki, Yasuhiro Shibata, Hideyuki Kamisawa, Satoshi Kurokawa, Akihiro Nakane, Kentaro Mizuno, Toshiki Kato, Tetsuji Maruyama, Yoshiyuki Kojima, Kenjiro Kohri
Organizer
American Urological Association Annual Meeting 2014
Place of Presentation
Orlando(USA)
Year and Date
2014-05-16 – 2014-05-21
Related Report
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[Presentation] Jarid1a regulates early stage spermatogenesis by demethylating histone H3 lysine 42013
Author(s)
Nishio Hidenori, Mizuno Kentaro, Kurokawa Satoshi, Kamisawa Hideyuki, Imura Makoto, Moritoki Yoshinobu, Shibata Yasuhiro, Nakane Akihiro, Kato Toshiki, Maruyama Tetsuji, Kojima Yoshiyuki, Hayashi Yutaro, Kohri Kenjiro
Organizer
The Society for Pediatric Urology 61th Annual Meeting
Place of Presentation
San Diego Convention Center( San Diego, USA)
Year and Date
20130503-20130505
Related Report
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