| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Outline of Final Research Achievements |
Klinefelter syndrome (KS) is associated with infertility due to non-obstructive azoospermia (NOA). The mechanism underlying NOA is still poorly understood. The generation of induced pluripotent stem (iPS) cells derived from KS patients may be useful for studying the disease mechanism and identifying novel therapies. Cells from KS patients were transduced with Sendai viral vectors encoding four transcription factors, OCT4, SOX2, KLF4, and C-MYC, and the transduced cells were analyzed for in vitro and in vivo pluripotency. KS patient-derived iPS cells were successfully generated and shown to produce teratomas in the testes of SCID mice. In vitro cardiac differentiation of the iPS cells was confirmed by the presence of clusters of beating cells.High quality KS patient-derived iPS cells with cardiomyocyte-differentiating ability were established.
|
