| Project/Area Number |
24791675
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 遺伝子 / 癌 / 発現制御 / 前立腺癌 / ポリアミド / 融合遺伝子 |
|---|
| Research Abstract |
We developed a novel compounds, which controls a prostate specific fusion gene TMPSS2-ERG. By using Synthetic pyrrole-imidazole (PI) polyamides, that recognize and attach to the minor groove of DNA with high affinity and specificity, we examined the effects of a TMPRSS2-ERG translocation breakpoints-targeted PI polyamide on prostate cancer. Human prostate cancer cell line LNCaP which has TMPRSS2-ERG by DHT stimulation or genotoxic stress was used for analysis. Treatment of this polyamide showed the repression of chromosomal translocation and TMPRSS2-ERG expression in LNCaP. In addition, this polyamide decreased cell growth rate and cell migration.
Moreover, we designed polyamides targeting for androgen receptor coregulator binding motif.
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