| Project/Area Number |
24791700
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMAGUCHI Ken 京都大学, 医学(系)研究科(研究院), 助教 (20378772)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
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| Keywords | 卵巣明細胞腺癌 / 代謝 / アミノ酸トランスポーター / グルタチオン / 酸化ストレス |
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| Research Abstract |
To elucidate HNF1B induces metabolic activities in ovarian clear cell carcinoma (CCC) cells, we assessed comprehensive metabolomic analyses using CE-TOFMS(capillary electrophoresis time-of-flight mass spectrometry) between HNF1B knockdown CCC cell line and control cell line. HNF1B changed glucose metabolism and caused aerobic glucolysis that was well known as the Warburg effect in CCC cells. HNF1B also increased intracellular glutathione levels through a cystine transporter, SLC3A1, expression. Intracellular glutathione levels and reactive oxygen species (ROS) were significantly decreased in SLC3A1 knockdown cells.
These results suggest that HNF1B contribute to resistance to anti-oxidative stress through the Warburg effect and intracellular glutathione in ovarian clear cell carcinoma.
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