Search for the genes involved in the development of uterine leiomyoma

• Project/Area Number: 24791704
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Yamaguchi University
• Principal Investigator: ASADA Hiromi 山口大学, 医学部附属病院, 助教 (90526906)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 生殖医学 / 子宮筋腫

Research Abstract

Profiles of genome-wide DNA methylation and mRNA expression were investigated in leiomyomas and in myometrium with and without leiomyomas. We identified 625 genes whose DNA methylation and mRNA expression patterns differed between leiomyomas and the adjacent myometrium. We transduced 3 genes (NRG1, NR3C1 and MAPK10) of 76 genes we identified by upstream factor analysis each in lentivirus-vectors. We will transduce these vectors in immortalized human uterine smooth muscle cell and transplant them under the kidney capsule in Immunodeficient mice.

Research Products

• [Journal Article] Potential Mechanisms of Aberrant DNA Hypomethylation on the X Chromosome in Uterine Leiomyomas (2014)
  • Author(s): S.Sato, R.Maekawa, Y.Yamagata, H.Asada (他5名)
  • Journal Title: J Reprod Dev Volume: 60 Pages: 47-54
  • NAID: 130003381861
  • Peer Reviewed
• [Journal Article] Importance of C/EBP β binding and histone acetylation status in the promoter regions for induction of IGFBP-1, PRL, and Mn-SOD by cAMP in human endometrial stromal cells (2014)
  • Author(s): I. Tamura, S. Sato, M. Okada, M. Tanabe, L. Lee, R. Maekawa, H. Asada (他 3名)
  • Journal Title: Endocrinology Volume: 155 Pages: 275-286
  • Peer Reviewed
• [Journal Article] Genome-wide DNA methylation analysis reveals a potential mechanism for the pathogenesis and development of uterine leiomyomas. (2013)
  • Author(s): Maekawa R, Sato S, Yamagata Y, Asada H, Tamura I, Lee L, Okada M, Tamura H, Takaki E, Nakai A, Sugino N
  • Journal Title: PLoS One Volume: 8 Issue: 6 Pages: e66632-e66632
  • DOI: 10.1371/journal.pone.0066632
  • NAID: 120005652007
  • Peer Reviewed
• [Journal Article] Changes in histone modification and DNA methylation of the StAR and Cyp19a1 promoter regions in granulosa cells undergoing luteinization during ovulation in female rats (2013)
  • Author(s): L. lee, H. Asada (他8名)
  • Journal Title: Endocrinology Volume: 154 Pages: 458-470
  • Peer Reviewed
• [Journal Article] Induction of IGFBP-1 expression by cAMP is associated with histone acetylation status of the oromoter region in human endometrial stromal cells (2012)
  • Author(s): I. Tamura, H. Asada (他7名)
  • Journal Title: Endocrinology Volume: 153 Pages: 5612-5621
  • Peer Reviewed
• [Presentation] 子宮筋腫における X 染色体の低メチル化変異機構 (2013)
  • Author(s): 佐藤 俊, 前川 亮, 山縣芳明, 浅田裕美, 田村 功, 李 理華, 岡田真紀, 田村博史, 杉野法広
  • Organizer: 第18回日本生殖内分泌学会学術集会
  • Place of Presentation: 東京.シェーンバッハ・サボー
  • Year and Date: 2013-12-07
• [Presentation] 子宮筋腫のゲノムワイドDNAメチル化解析 (2012)
  • Author(s): 前川 亮, 山縣芳明, 浅田裕美, 田村 功, 李 理華, 田邊 学, 田村博史, 杉野法広
  • Organizer: 第85回日本内分泌学会
  • Place of Presentation: 愛知.名古屋国際会議場
  • Year and Date: 2012-04-19
• [Presentation] 子宮筋腫のゲノムワイドDNAメチル化解析 (2012)
  • Author(s): 前川 亮、山縣 芳明、田村 功、李 理華、浅田 裕美、杉野 法広
  • Organizer: 第64回日本産科婦人科学会
  • Place of Presentation: 神戸（神戸ポートピアホテル）