The underlying clinical study on production mechanism of the new endometriosis marker

• Project/Area Number: 24791707
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Ehime University
• Principal Investigator: NABETA Motowo 愛媛大学, 医学部附属病院, 講師 (80444751)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 子宮内膜症 / 抗PDIK1L自己抗体 / 血清診断 / 自己抗体

Research Abstract

[purpose] We searched for an endometriotic new serodiagnosis marker.[method] We established the ELISA method and analyzed it.We analyzed the tissue manifestation nature of the PDIK1L protein by immunity tissue staining.
The diagnosability (58.1% of sensitivity, specificity 84.1%) of the antiPDIK1L self antibody titers was clearly superior in serum for the CA125 which was an existing serum marker. [results and discussion] Furthermore, we were able to put up the sensitivity of the endometriosis diagnosis to 73.0% by using both together. As a result of having analyzed it by immunity tissue staining, we were strong, and manifestation of PDIK1L was found in endometriosis tissue and developed in 94.7% of endometriosis cases.[conclusions] We found antiPDIK1L autoantibody as a new method of the endometriosis diagnosis.

Research Products

• [Journal Article] Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma (2013)
  • Author(s): Fujioka T, Yasuoka T, Koizumi M, Tanaka H, Hashimoto H, Nabeta M, Koizumi K, Matsubara Y, Hamada K, Matsubara K, Katayama T, Nawa A
  • Journal Title: Mol Clin Oncol Volume: 1(1) Pages: 165-170
  • Peer Reviewed
• [Journal Article] Differential expressions of toll-like receptor genes in the vagina of pregnant mice (2013)
  • Author(s): Takeuchi T, Yoshida M, Shimizu T, Asano A, Shimokawa T, Nabeta M, Usui T
  • Journal Title: J Vet Med Sci Volume: 75(5) Pages: 561-5
  • Peer Reviewed
• [Journal Article] Gene Transcriptions of Toll-Like Receptors in the Mouse Uterus during Gestation (2013)
  • Author(s): Takeuchi T, Sugimoto Y, Asano A, Shimokawa T, Nabeta M, Usui T
  • Journal Title: J Vet Med Sci Volume: 75(5) Pages: 547-51
  • Peer Reviewed
• [Journal Article] Sugar expression in the mucosae of the canine uterus and vagina during the oestrous cycle and with pyometra (2013)
  • Author(s): Y. Yasunaga, T. Takeuchi, T. Shimokawa, A. Asano, M. Nabeta, Y. Ohta
  • Journal Title: The Veterinary Journal Volume: 196 Pages: 116-118
  • Peer Reviewed
• [Journal Article] Nucleated red blood cells are involved in endothelial progenitor cell proliferation in umbilical venous blood of preeclamptic patients (2013)
  • Author(s): Uchikura Y., Matsubara K., Matsubara Y., Mori M., Nabeta M., Hashimoto H., Fujioka T., Hamada K., Nawa A
  • Journal Title: Hypertension Research in Pregnancy Volume: 1 Pages: 46-51
  • Peer Reviewed
• [Journal Article] Concurrent chemoradiotherapy with nedaplatin in patients with stage IIA to IVA cervical carcinoma. (2013)
  • Author(s): Fujioka, T., Yasuoka, T., Koizumi, M., Tanaka, H., Hashimoto, H., Nabeta, M., Koizumi, K., Matsubara, Y., Hamada, K., Matsubara, K., Katayama, T., Nawa, A.
  • Journal Title: Molecular and clinical oncology Volume: 1 Issue: 1 Pages: 165-170
  • DOI: 10.3892/mco.2012.27
  • Peer Reviewed
• [Journal Article] Differential Expressions of Toll-Like Receptor Genes in the Vagina of Pregnant Mice (2013)
  • Author(s): Takeuchi T, Yoshida M, Shimizu T, Asano A, Shimokawa T, Nabeta M, Usui T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 5 Pages: 561-565
  • DOI: 10.1292/jvms.12-0458
  • NAID: 130002502173
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Gene Transcriptions of Toll-Like Receptors in the Mouse Uterus during Gestation (2013)
  • Author(s): Takeuchi T, Sugimoto Y, Asano A, Shimokawa T, Nabeta M, Usui T.
  • Journal Title: Journal of Veterinary Medical Science Volume: 75 Issue: 5 Pages: 547-551
  • DOI: 10.1292/jvms.12-0457
  • NAID: 130002502169
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed
• [Journal Article] Sugar Expressions on the Vaginal Epithelium in Pregnant Mice (2013)
  • Author(s): Youhei YASUNAGA, Takashi TAKEUCHI, Tetsuya SHIMOKAWA, Motowo NABETA, Aya MATSUU, Atsushi ASANO and Yasuhiko OHTA
  • Journal Title: The Journal of Veterinary Medical Science Volume: 74 Pages: 805-808
  • Peer Reviewed
• [Journal Article] Gene Transcriptions of Toll-Like Receptors in the Mouse Uterus during Gestation (2012)
  • Author(s): T. TAKEUCHI, Y. SUGIMOTO, A. ASANO, T. SHIMOKAWA, M. NABETA and T. USUI
  • Journal Title: J Vet Med Sci Volume: 75 Pages: 547-551
  • Peer Reviewed
• [Journal Article] Differential Expressions of Toll-Like Receptor Genes in the Vagina of Pregnant Mice (2012)
  • Author(s): Takeuchi T, Yoshida M, Shimizu T, Asano A, Shimokawa T, Nabeta M, Usui T.
  • Journal Title: J Vet Med Sci. 2012 Volume: 75 Pages: 561-565
  • Peer Reviewed
• [Journal Article] 全腹腔鏡下子宮全摘術における縫合結紮トレーニング機器の開発 (2012)
  • Author(s): 藤岡徹、安岡稔晃、髙木香津子、萩山容子、井上彩、小泉絵理、内倉友香、田中寛希、森 美妃、小泉雅江、橋本尚、小泉幸司、鍋田基生、松原裕子、濱田雄行、松原圭一、那波明宏
  • Journal Title: 日本産科婦人科内視鏡学会雑誌 Volume: 28 Pages: 650-655
  • NAID: 130003374540
  • Peer Reviewed
• [Presentation] 愛媛大学医学部附属病院における不妊診療の現況 (2013)
  • Author(s): 鍋田基生, 伊木朱有美, 佐保知織, 安岡稔晃, 小泉絵理, 井上彩, 内倉友香, 田中寛希, 森美妃, 高木香津子, 近藤恵美, 橋本尚, 小泉雅江, 松元隆, 松原裕子, 濱田雄行, 藤岡徹, 松原圭一, 那波明宏
  • Organizer: 第22回愛媛県産婦人科医会臨床集談会
  • Place of Presentation: 松山市
  • Year and Date: 2013-11-23
• [Presentation] 子宮内膜症に特異的な自己抗原タンパク質 PDIK1L の組織発現性検討 (2013)
  • Author(s): 鍋田基生, 伊木朱有美, 佐保知織, 小泉絵理, 井上彩, 森美妃, 高木香津子, 近藤恵美, 小泉雅江, 濱田雄行, 松原圭一, 草薙康城, 那波明宏
  • Organizer: 第58回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸市
• [Presentation] Analysis of the anti-STX5 autoantibody identified as a new serum marker for endometriosis (2013)
  • Author(s): Nabeta M, Iki A, Hashimoto H, Koizumi M, Matsubara Y, Hamada K, Fujioka T, Matsubara K, Kusanagi Y, Nawa A
  • Organizer: Annual meeting of ESHRE (European Society of Human Reproduction and Embryology)
  • Place of Presentation: London, United Kingdom
• [Presentation] 子宮内膜症に特異的な自己抗原タンパク質PDIK1Lの組織発現性検討 (2013)
  • Author(s): 鍋田基生、伊木朱有美、佐保知織、小泉絵理、井上彩、森美妃、高木香津子、近藤恵美、小泉雅江、濱田雄行、松原圭一、草薙康城、那波明宏
  • Organizer: 第58回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸市
• [Presentation] Analysis of the anti-STX5 autoantibody identified as a new serum marker for endometriosis. (2013)
  • Author(s): Nabeta M, Iki A, Hashimoto H, Koizumi M, Matsubara Y, Hamada K, Fujioka T, Matsubara K, Kusanagi Y, Nawa A.
  • Organizer: Annual meeting of ESHRE (European Society of Human Reproduction and Embryology)
  • Place of Presentation: London, UK
• [Presentation] 新たな子宮内膜症マーカーとしての自己抗原タンパク質PDIK1Lに対する組織発現性の検討 (2013)
  • Author(s): 鍋田基生、伊木朱有美、片山英司、曽我美子、杉田敦郎、草薙康城、阿部康人、
  • Organizer: 第34回日本エンドメトリオーシス学会
  • Place of Presentation: 宇都宮市
• [Presentation] Identification of anti-PDIK1L autoantibody as a novel serum marker for endometriosis (2012)
  • Author(s): Iki A., Nabeta M., Inoue A., Kusanagi Y., Nawa A.
  • Organizer: 2nd Asian Conference on Endometriosis (ACEII)
  • Place of Presentation: Istanbul, Turky
• [Presentation] 新規子宮内膜症マーカーとして同定した自己抗原タンパク質PDIK1Lの組織発現性の検討 (2012)
  • Author(s): 鍋田基生、伊木朱有美、佐保知織、井上彩、小泉絵理、小泉幸司、濱田雄行、草薙康城、阿部康人、那波明宏
  • Organizer: 第30回日本受精着床学会総会・学術講演会
  • Place of Presentation: 大阪市
• [Presentation] 新規の子宮内膜症マーカーである抗STX5自己抗体を用いた子宮内膜症早期診断の (2012)
  • Author(s): 鍋田基生、森美妃、小泉幸司、片山博子、濱田雄行、草薙康城、那波明宏
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸市
• [Presentation] 愛媛大学医学部附属病院における不妊診療の現況
  • Author(s): 鍋田基生、伊木朱有美、佐保知織、安岡稔晃、小泉絵理、井上彩、内倉友香、田中寛希、森美妃、高木香津子、近藤恵美、橋本尚、小泉雅江、松元隆、松原裕子、濱田雄行、 藤岡徹、松原圭一、那波明宏
  • Organizer: 第２２回愛媛県産婦人科医会臨床集談会
  • Place of Presentation: 松山市
• [Book] 若手の最新研究紹介コーナー「新規子宮内膜症マーカーの産生メカニズムに関する基礎的・臨床的研究」 産科と婦人科 (2013)
  • Author(s): 鍋田基生
• [Book] 産科と婦人科 11号（99） 若手の最新研究紹介コーナー 「新規子宮内膜症マーカーの産生メカニズムに関する基礎的・臨床的研究」 （2012～2013年度 文部科学省科学研究費補助金採択研究） (2013)
  • Author(s): 鍋田基生
  • Publisher: 診断と治療社