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Measurement of HTLV-1 provirus loads in pregnant women with HTLV-1 carrier

Research Project

Project/Area Number 24791712
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionNagasaki University

Principal Investigator

MIURA Shoko  長崎大学, 医歯(薬)学総合研究科, 研究員 (00404301)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords母子感染 / HTLV-1 / スクリーニング検査 / PCR検査 / 胎内感染 / 妊娠 / ウイルス量 / 妊婦 / キャリア / スクリーニング / ウエスタンブロット / 判定保留 / PCR / 臍帯血
Research Abstract

CR primers for HTLV-1genome were located at the pX region, while PCR primers for beta-globin as an internal control were on exon 2. HTLV-1 proviral load was calculated by the formula [(HTLV-1 pX copy number)/(beta-globin copy number/2)] x10, 000 cells. 1) Quantitative PCR test of HTLV-1 infection is useful for the HTLV-1 screening system in pregnant women. 2) The proviral load of HTLV-1 genome in blood samples was decreased significantly after delivery. However, the changes of HTLV-1 proviral load may not reflect the changes of CD4+CD25+T cells in pregnant women.3)In six pregnant women with HTLV-1 carrier, HTLV-1 provirus was detected in umbilical blood cells, suggesting that the intrauterine infection may be one of the mother-to-child transmitted infection of HTLV-1.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 3 results) Presentation (2 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome2014

    • Author(s)
      Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H
    • Journal Title

      Prenat Diagn

      Volume: 34 Pages: 345-349

    • Related Report
      2013 Final Research Report
  • [Journal Article] Identification of Endometrioid Endometrial Carcinoma-associated microRNAs in Tissue and Plasma2014

    • Author(s)
      Tsukamoto O, Miura K, Mishima H, Abe S, Kaneuchi M, Higashijima A, Miura S, Kinoshita A, Yoshiura KI, Masuzaki H
    • Journal Title

      Gynecologic Oncology

      Volume: 132 Pages: 715-21

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome2014

    • Author(s)
      Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H.
    • Journal Title

      Prenatal Diagnosis

      Volume: 34 Issue: 4 Pages: 345-349

    • DOI

      10.1002/pd.4307

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy2013

    • Author(s)
      Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S, Hasegawa Y, Miura S, Yamasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
    • Journal Title

      Prenatal Diagnosis

      Volume: 33 Pages: 214-222

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer2013

    • Author(s)
      Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Sasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
    • Journal Title

      J Hum Genet

      Volume: 58 Pages: 250-253

    • NAID

      10031177218

    • Related Report
      2013 Final Research Report
  • [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix2013

    • Author(s)
      Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H
    • Journal Title

      J Med Virol

      Volume: 85 Pages: 2093-2100

    • Related Report
      2013 Final Research Report
  • [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix2013

    • Author(s)
      Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H
    • Journal Title

      Journal of Medical Virology

      Volume: 85 Issue: 12 Pages: 2093-2100

    • DOI

      10.1002/jmv.23709

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer.2013

    • Author(s)
      Abe, S, Miura, K, Kinoshita, A, Mishima, H, Miura, S, Yamasaki, K, Hasegawa, Y, Higashijima, A, Jo, O, Sasaki, K, Yoshida, A, Yoshiura, KI, Masuzaki.
    • Journal Title

      Journal of Human Genetics

      Volume: 58 Issue: 5 Pages: 250-253

    • DOI

      10.1038/jhg.2013.7

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders2011

    • Author(s)
      Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, and Masuzaki H
    • Journal Title

      Journal of Human Genetics

      Volume: 56 Pages: 296-299

    • NAID

      10030658849

    • Related Report
      2013 Final Research Report
  • [Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women2011

    • Author(s)
      Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, and Masuzaki H
    • Journal Title

      Journal of Human Genetics

      Volume: 56 Pages: 313-315

    • NAID

      10030658951

    • Related Report
      2013 Final Research Report
  • [Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology2011

    • Author(s)
      Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 37 Pages: 1666-1670

    • NAID

      120006985461

    • Related Report
      2013 Final Research Report
  • [Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US

    • Author(s)
      Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura KI and Masuzaki H
    • Journal Title

      Journal of Human Genetics

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Clinical Applications of Analysis of Plasma Circulating Complete Hydatidiform Mole Pregnancy-Associated miRNAs in Persistent Gestational Trophoblastic Disease

    • Author(s)
      Miura K, Hasegawa Y, Abe S, Higashijima A, Miura S, Mishima H, Kinoshita A, Kaneuchi M, Yoshiura KI, Masuzaki H
    • Journal Title

      Placenta

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Journal Article] Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight

    • Author(s)
      Miura K, Morisaki S, Abe S, Higashijima A, Hasegawa Y, Miura S, Tateishi S, Mishima H, Yoshiura KI, Masuzaki H
    • Journal Title

      Placenta

      Volume: (in press)

    • Related Report
      2013 Final Research Report
  • [Presentation] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer2013

    • Author(s)
      Kiyonori Miura, Shuhei Abe, Akira Kinoshita, Hiroyuki Mishima, Shoko Miura, Koh-ichiro Yoshiura, and Hideaki Masuzaki
    • Organizer
      63th Annual meeting of American Society of Human Genetics
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Koh-ichiro Yoshiura and Hideaki Masuzaki Identification of Pregnancy-Associated microRNAs in Maternal Plasma2012

    • Author(s)
      Kiyonori Miura, Ai Higashijima, Shoko Miura, Kentaro Yamasaki, Ozora Jo, Shuhei Abe , Yuri Hasegawa
    • Organizer
      The 16th International Conference on Prenatal Diagnosis and Therapy
    • Place of Presentation
      Maiami, Florida, USA
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2013 Final Research Report
  • [Remarks] 長崎大学医学部産婦人科

    • URL

      http://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] 胎盤機能の網羅的かつ非侵襲的評価方法および検査用試薬2014

    • Inventor(s)
      三浦清徳, 増崎英明, 新川詔夫, 吉浦孝一郎, 山崎健太郎, 三浦生子
    • Industrial Property Rights Holder
      国立大学法人 長崎大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2014-03-07
    • Related Report
      2013 Final Research Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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