Measurement of HTLV-1 provirus loads in pregnant women with HTLV-1 carrier

• Project/Area Number: 24791712
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: MIURA Shoko 長崎大学, 医歯(薬)学総合研究科, 研究員 (00404301)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 母子感染 / HTLV-1 / スクリーニング検査 / PCR検査 / 胎内感染 / 妊娠 / ウイルス量 / 妊婦 / キャリア / スクリーニング / ウエスタンブロット / 判定保留 / PCR / 臍帯血

Research Abstract

CR primers for HTLV-1genome were located at the pX region, while PCR primers for beta-globin as an internal control were on exon 2. HTLV-1 proviral load was calculated by the formula [(HTLV-1 pX copy number)/(beta-globin copy number/2)] x10, 000 cells. 1) Quantitative PCR test of HTLV-1 infection is useful for the HTLV-1 screening system in pregnant women. 2) The proviral load of HTLV-1 genome in blood samples was decreased significantly after delivery. However, the changes of HTLV-1 proviral load may not reflect the changes of CD4+CD25+T cells in pregnant women.3)In six pregnant women with HTLV-1 carrier, HTLV-1 provirus was detected in umbilical blood cells, suggesting that the intrauterine infection may be one of the mother-to-child transmitted infection of HTLV-1.

Research Products

• [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome (2014)
  • Author(s): Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H
  • Journal Title: Prenat Diagn Volume: 34 Pages: 345-349
• [Journal Article] Identification of Endometrioid Endometrial Carcinoma-associated microRNAs in Tissue and Plasma (2014)
  • Author(s): Tsukamoto O, Miura K, Mishima H, Abe S, Kaneuchi M, Higashijima A, Miura S, Kinoshita A, Yoshiura KI, Masuzaki H
  • Journal Title: Gynecologic Oncology Volume: 132 Pages: 715-21
• [Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome (2014)
  • Author(s): Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H.
  • Journal Title: Prenatal Diagnosis Volume: 34 Issue: 4 Pages: 345-349
  • DOI: 10.1002/pd.4307
  • Peer Reviewed
• [Journal Article] Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy (2013)
  • Author(s): Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S, Hasegawa Y, Miura S, Yamasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
  • Journal Title: Prenatal Diagnosis Volume: 33 Pages: 214-222
• [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer (2013)
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Sasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
  • Journal Title: J Hum Genet Volume: 58 Pages: 250-253
  • NAID: 10031177218
• [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix (2013)
  • Author(s): Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H
  • Journal Title: J Med Virol Volume: 85 Pages: 2093-2100
• [Journal Article] Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix (2013)
  • Author(s): Hamaguchi D, Miura K, Abe S, Kinoshita A, Miura S, Yamasaki K, Yoshiura K, Masuzaki H
  • Journal Title: Journal of Medical Virology Volume: 85 Issue: 12 Pages: 2093-2100
  • DOI: 10.1002/jmv.23709
  • Peer Reviewed
• [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer. (2013)
  • Author(s): Abe, S, Miura, K, Kinoshita, A, Mishima, H, Miura, S, Yamasaki, K, Hasegawa, Y, Higashijima, A, Jo, O, Sasaki, K, Yoshida, A, Yoshiura, KI, Masuzaki.
  • Journal Title: Journal of Human Genetics Volume: 58 Issue: 5 Pages: 250-253
  • DOI: 10.1038/jhg.2013.7
  • Peer Reviewed
• [Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders (2011)
  • Author(s): Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, and Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: 56 Pages: 296-299
  • NAID: 10030658849
• [Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, and Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: 56 Pages: 313-315
  • NAID: 10030658951
• [Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 37 Pages: 1666-1670
  • NAID: 120006985461
• [Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura KI and Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: (in press)
• [Journal Article] Clinical Applications of Analysis of Plasma Circulating Complete Hydatidiform Mole Pregnancy-Associated miRNAs in Persistent Gestational Trophoblastic Disease
  • Author(s): Miura K, Hasegawa Y, Abe S, Higashijima A, Miura S, Mishima H, Kinoshita A, Kaneuchi M, Yoshiura KI, Masuzaki H
  • Journal Title: Placenta Volume: (in press)
• [Journal Article] Circulating levels of maternal plasma cell-free pregnancy-associated placenta-specific microRNAs are associated with placental weight
  • Author(s): Miura K, Morisaki S, Abe S, Higashijima A, Hasegawa Y, Miura S, Tateishi S, Mishima H, Yoshiura KI, Masuzaki H
  • Journal Title: Placenta Volume: (in press)
• [Presentation] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer (2013)
  • Author(s): Kiyonori Miura, Shuhei Abe, Akira Kinoshita, Hiroyuki Mishima, Shoko Miura, Koh-ichiro Yoshiura, and Hideaki Masuzaki
  • Organizer: 63th Annual meeting of American Society of Human Genetics
  • Place of Presentation: Boston, USA
• [Presentation] Koh-ichiro Yoshiura and Hideaki Masuzaki Identification of Pregnancy-Associated microRNAs in Maternal Plasma (2012)
  • Author(s): Kiyonori Miura, Ai Higashijima, Shoko Miura, Kentaro Yamasaki, Ozora Jo, Shuhei Abe , Yuri Hasegawa
  • Organizer: The 16th International Conference on Prenatal Diagnosis and Therapy
  • Place of Presentation: Maiami, Florida, USA
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/
• [Remarks] 長崎大学医学部産婦人科
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/
• [Patent(Industrial Property Rights)] 胎盤機能の網羅的かつ非侵襲的評価方法および検査用試薬 (2014)
  • Inventor(s): 三浦清徳, 増崎英明, 新川詔夫, 吉浦孝一郎, 山崎健太郎, 三浦生子
  • Industrial Property Rights Holder: 国立大学法人 長崎大学
  • Industrial Property Rights Type: 特許
  • Acquisition Date: 2014-03-07