Measurement of HTLV-1 provirus loads in pregnant women with HTLV-1 carrier
Project/Area Number |
24791712
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Obstetrics and gynecology
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Research Institution | Nagasaki University |
Principal Investigator |
MIURA Shoko 長崎大学, 医歯(薬)学総合研究科, 研究員 (00404301)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 母子感染 / HTLV-1 / スクリーニング検査 / PCR検査 / 胎内感染 / 妊娠 / ウイルス量 / 妊婦 / キャリア / スクリーニング / ウエスタンブロット / 判定保留 / PCR / 臍帯血 |
Research Abstract |
CR primers for HTLV-1genome were located at the pX region, while PCR primers for beta-globin as an internal control were on exon 2. HTLV-1 proviral load was calculated by the formula [(HTLV-1 pX copy number)/(beta-globin copy number/2)] x10, 000 cells. 1) Quantitative PCR test of HTLV-1 infection is useful for the HTLV-1 screening system in pregnant women. 2) The proviral load of HTLV-1 genome in blood samples was decreased significantly after delivery. However, the changes of HTLV-1 proviral load may not reflect the changes of CD4+CD25+T cells in pregnant women.3)In six pregnant women with HTLV-1 carrier, HTLV-1 provirus was detected in umbilical blood cells, suggesting that the intrauterine infection may be one of the mother-to-child transmitted infection of HTLV-1.
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Report
(3 results)
Research Products
(19 results)
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[Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome2014
Author(s)
Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H
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Journal Title
Prenat Diagn
Volume: 34
Pages: 345-349
Related Report
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[Journal Article] Predominantly placenta-expressed mRNAs in maternal plasma as predictive markers for twin-twin transfusion syndrome2014
Author(s)
Miura K, Higashijima A, Miura S, Mishima H, Yamasaki K, Abe S, Hasegawa Y, Kaneuchi M, Yoshida A, Kinoshita A, Yoshiura KI, Masuzaki H.
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Journal Title
Prenatal Diagnosis
Volume: 34
Issue: 4
Pages: 345-349
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy2013
Author(s)
Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S, Hasegawa Y, Miura S, Yamasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
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Journal Title
Prenatal Diagnosis
Volume: 33
Pages: 214-222
Related Report
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[Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer2013
Author(s)
Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Sasaki K, Yoshida A, Yoshiura KI, and Masuzaki H
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Journal Title
J Hum Genet
Volume: 58
Pages: 250-253
NAID
Related Report
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[Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer.2013
Author(s)
Abe, S, Miura, K, Kinoshita, A, Mishima, H, Miura, S, Yamasaki, K, Hasegawa, Y, Higashijima, A, Jo, O, Sasaki, K, Yoshida, A, Yoshiura, KI, Masuzaki.
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Journal Title
Journal of Human Genetics
Volume: 58
Issue: 5
Pages: 250-253
DOI
Related Report
Peer Reviewed
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[Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders2011
Author(s)
Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, and Masuzaki H
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Journal Title
Journal of Human Genetics
Volume: 56
Pages: 296-299
NAID
Related Report
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[Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women2011
Author(s)
Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, and Masuzaki H
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Journal Title
Journal of Human Genetics
Volume: 56
Pages: 313-315
NAID
Related Report
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[Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology2011
Author(s)
Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
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Journal Title
Journal of Obstetrics and Gynaecology Research
Volume: 37
Pages: 1666-1670
NAID
Related Report
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[Journal Article] Single human papillomavirus 16 or 52 infection and later cytological findings in Japanese women with NILM or ASC-US
Author(s)
Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y, Higashijima A, Jo O, Yoshida A, Kaneuchi M, Yoshiura KI and Masuzaki H
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Journal Title
Journal of Human Genetics
Volume: (in press)
Related Report
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