| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
In vivo reconstitution system of human fallopian tube (hFT) is needed to identify and characterize stem cells of hFT in vivo. Using immortalized human endometrial cells, we here attempted to obtain fundamental knowledge and techniques to realize the hFT reconstitution and cell tracking system. For this purpose, we first made endometrial cell lines stably expressing bioluminescent and fluorescent proteins. We then developed a method to introduce genes of interest into cells without cell culture and established a cell tracking system through the combinatory use of our labeling method and a tissue reconstitution system. The results of this study will be useful for identification and characterization of putative hFT stem cells when a hFT cell line becomes available.
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