| Project/Area Number |
24791729
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Niigata University (2013)
National Center of Neurology and Psychiatry (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 癌幹細胞 / 卵巣がん / 卵巣がん幹細胞 / 分化可塑性 / がん幹細胞 |
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| Research Abstract |
Cancer stem cells (CSC) can self-renew, differentiate and recapitulate the tumors. In the absence of serum, CSC from some solid tumors are capable of proliferation in vitro under sphere-forming conditions. We have established spheroid culture conditions for cultivating ovarian cancer cells derived from surgical specimen. These spheroid cells are tumorigenic when explanted into immunodeficient mice, and immuno-histological analysis indicates that the resulting tumors are indistinguishable from the originals. When serum is added to the in vitro culture media, the spheroid cells differentiate. shRNA-mediated knockdown of stemness factor inhibited cell proliferation.
The differentiated cells, when returned to the sphere-forming serum-free conditions, were capable of reforming spheroids and re-expression of the stem cell markers.
These findings indicated that the differentiation of the spheroid stem cells was highly reversible.
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