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Analysis of the role of stemness factor in ovarian cancer stem cell

Research Project

Project/Area Number 24791729
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Obstetrics and gynecology
Research InstitutionNiigata University (2013)
National Center of Neurology and Psychiatry (2012)

Principal Investigator

ISHIGURO Tatsuya  新潟大学, 医歯学総合病院, 助教 (80625690)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords癌幹細胞 / 卵巣がん / 卵巣がん幹細胞 / 分化可塑性 / がん幹細胞
Research Abstract

Cancer stem cells (CSC) can self-renew, differentiate and recapitulate the tumors. In the absence of serum, CSC from some solid tumors are capable of proliferation in vitro under sphere-forming conditions. We have established spheroid culture conditions for cultivating ovarian cancer cells derived from surgical specimen. These spheroid cells are tumorigenic when explanted into immunodeficient mice, and immuno-histological analysis indicates that the resulting tumors are indistinguishable from the originals. When serum is added to the in vitro culture media, the spheroid cells differentiate. shRNA-mediated knockdown of stemness factor inhibited cell proliferation.
The differentiated cells, when returned to the sphere-forming serum-free conditions, were capable of reforming spheroids and re-expression of the stem cell markers.
These findings indicated that the differentiation of the spheroid stem cells was highly reversible.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2013 2012

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (4 results)

  • [Journal Article] Differential expression of nanog1 and nanogp8 in colon cancer cells2012

    • Author(s)
      Tatsuya Ishiguro, Ai Sato, Hirokazu Ohata, Hiroaki Sakai, Hitoshi Nakagama, Koji Okamoto
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 418 Pages: 199-204

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] miR-493 induction during carcinogenesis blocksmetastatic settlement of colon cancer cells in liver2012

    • Author(s)
      Koji Okamoto, Tatsuya Ishiguro, Yutaka Midorikawa, Hirokazu Ohata, Masashi Izumiya, Naoto Tsuchiya, Ai Sato, Hiroaki Sakai, Hitoshi Nakagama
    • Journal Title

      The EMBO Journal

      Volume: 31 Pages: 1752-1763

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of the Stem-like Cell Regulator CD44 by Rho Kinase Inhibition Contributes to the Maintenance of Colon Cancer -Initiating Cells2012

    • Author(s)
      Hirokazu Ohata, Tatsuya Ishiguro, Yuki Aihara, Ai Sato, Hiroaki Sakai, Shigeki Sekine, Hirokazu Taniguchi, Takayuki Akasu, Shin Fujita, Hitoshi Nakagama, and Koji Okamoto
    • Journal Title

      Cancer Res

      Volume: 72 Pages: 5101-5110

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] in vitro cultivation and characterization of cancer stem cells derived from human ovarian cancer2013

    • Author(s)
      Tatsuya Ishiguro, Hirokazu Ohata, Hitoshi Tsuda, Takahiro Kasamatsu, Takayuki Enomoto, Kenichi Tanaka, Hitoshi Nakagama, and Koji Okamoto
    • Organizer
      72nd Annual meeting of the Japanese Cancer Association 2013
    • Place of Presentation
      Yokohama
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト卵巣がん検体由来がん幹細胞のin vitro 培養系の確立とその生化学的特性の解明2013

    • Author(s)
      石黒竜也, 榎本隆之, 田中憲一
    • Organizer
      第65回日本産婦人科学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Final Research Report
  • [Presentation] in vitro cultivation and characterization of cancer stem cells derived from human ovarian cancer2013

    • Author(s)
      石黒竜也 大畑広和 津田均 笠松高弘 榎本隆之 田中憲一 中釜斉 岡本康司
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      横浜
    • Related Report
      2013 Annual Research Report
  • [Presentation] ヒト卵巣がん検体由来がん幹細胞のin vitro 培養系の確立とその生物学的特性の解析2013

    • Author(s)
      石黒竜也 榎本隆之 田中憲一
    • Organizer
      日本産婦人科学会学術総会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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