Identification of responsible genes associated with an acquired resistance anti-cancer drugs by exome sequence and microarray analyses in smoking exposed human cancers.

• Project/Area Number: 24791738
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: OGAWA Takenori 東北大学, 大学病院, 講師 (50375060)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 頭頸部癌 / 薬剤耐性 / 分子標的薬耐性 / 喫煙

Research Abstract

Acquired resistance to anti-cancer drugs by smoking related carcinomas is a persistent problem in many chemotherapy treatment programs. Currently, no model exists to compare parental and drug resistant cell lines in smoking related head and neck squamous cell carcinomas. In order to effectively study the mechanisms of resistance, we sought to develop a number of cell lines resistant to multiple drugs commonly used in the treatment of smoking related cancers. MTT assay revealed that all three lines were resistant to the EGFR targeted drugs and cis-diamminedichloroplatinum. The identification of the resistance-related genes was also conducted between parental cells and CSE exposed cells using sequencing technologies and microarray. This analyses revealed several genes that were differentially expressed between parental and subline cells. These novel drug resistant cells lines may help to identify gene products responsible for resistance to single and multiple agents.

Research Products

• [Journal Article] Human papillomavirus status of head and neck cancer as determined in cytologic specimens using the hybrid-capture 2 assay (2014)
  • Author(s): Smith DF, Maleki Z, Coughlan D, Gooi Z, Akpeng B, Ogawa T, Bishop JA, Frick KD, Agrawal N, Gourin CG, Ha PK, Koch WM, Richmon JD, Westra WH, Pai SI
  • Journal Title: Oral Oncol Volume: S1368-8375(14) Issue: 6 Pages: 47-5
  • DOI: 10.1016/j.oraloncology.2014.02.011
  • Peer Reviewed
• [Journal Article] Association between septal deviation and sinonasal papilloma (2013)
  • Author(s): Nomura K, Ogawa T, Sugawara M, Honkura Y, Oshima H, Arakawa K, Oshima T, Katori Y
  • Journal Title: Tohoku J Exp Med Volume: 231(4) Pages: 315-9
  • NAID: 130004822390
  • Peer Reviewed
• [Journal Article] Human papillomavirus-related carcinoma with adenoid cystic-like features : a peculiar variant of head and neck cancer restricted to the sinonasal tract (2013)
  • Author(s): Bishop JA, Ogawa T, Stelow EB, Moskaluk CA, Koch WM, Pai SI, Westra WH
  • Journal Title: Am J Surg Pathol Volume: 37(6) Pages: 836-44
  • Peer Reviewed
• [Journal Article] Human Papillomavirus-related Carcinomas of the Sinonasal Tract (2013)
  • Author(s): Bishop JA, Guo TW, Smith DF, Wang H, Ogawa T, Pai SI, Westra WH
  • Journal Title: Am J Surg Pathol Volume: 37(2) Pages: 185-92
  • Peer Reviewed
• [Journal Article] Molecular etiology of second primary tumors in contralateral tonsils of human papillomavirus-associated index tonsillar carcinomas (2013)
  • Author(s): Joseph AW, Ogawa T, Bishop JA, Lyford-Pike S, Chang X, Phelps TH, Westra WH, Pai SI
  • Journal Title: Oral Oncol Volume: 49(3) Pages: 244-8
  • Peer Reviewed
• [Journal Article] Human papillomavirus-related carcinoma with adenoid cystic-like features: a peculiar variant of head and neck cancer restricted to the sinonasal tract. (2013)
  • Author(s): Bishop JA, Ogawa T, Stelow EB, Moskaluk CA, Koch WM, Pai SI, Westra WH.
  • Journal Title: Am J Surg Pathol. Volume: 37(6) Issue: 6 Pages: 836-44
  • DOI: 10.1097/pas.0b013e31827b1cd6
  • Peer Reviewed
• [Journal Article] Association between septal deviation and sinonasal papilloma. (2013)
  • Author(s): Nomura K, Ogawa T, Sugawara M, Honkura Y, Oshima H, Arakawa K, Oshima T, Katori Y.
  • Journal Title: Tohoku J Exp Med. Volume: 231(4) Pages: 315-9
  • NAID: 130004822390
  • Peer Reviewed
• [Journal Article] Molecular etiology of second primary tumors in contralateral tonsils of human papillomavirus-associated index tonsillar carcinomas. (2013)
  • Author(s): Joseph AW, Ogawa T, Bishop JA, Lyford-Pike S, Chang X, Phelps TH, Westra WH, Pai SI.
  • Journal Title: Oral Oncol. Volume: 49(3) Pages: 244-8
  • Peer Reviewed
• [Journal Article] Human Papillomavirus-related Carcinomas of the Sinonasal Tract. (2013)
  • Author(s): Bishop JA, Guo TW, Smith DF, Wang H, Ogawa T, Pai SI, Westra WH.
  • Journal Title: Am J Surg Pathol. Volume: 37(2) Pages: 185-92
  • Peer Reviewed
• [Journal Article] Suprabasin is hypomethylated and associated with metastasis in salivary adenoid cystic carcinoma (2012)
  • Author(s): Shao C, Tan M, Bishop JA, Liu J, Bai W, Gaykalova DA, Ogawa T, Vikani AR, Agrawal Y, Li RJ, Kim MS, Westra WH, Sidransky D, Califano JA, Ha PK
  • Journal Title: PLoS One Volume: 7(11)
  • Peer Reviewed
• [Journal Article] Differences between oral cancer and cancers of the pharynx and larynx on a molecular level (2012)
  • Author(s): Shiga K, Ogawa T, Katagiri K, Yoshida F, Tateda M, Matsuura K, Kobayashi T
  • Journal Title: Oncol Lett Volume: 3(1) Pages: 238-243
  • Peer Reviewed
• [Journal Article] miR-34a is downregulated in cis-diamminedichloroplatinum treated sinonasal squamous cell carcinoma patients with poor prognosis (2012)
  • Author(s): Ogawa T, Saiki Y, Shiga K, Chen N, Fukushige S, Sunamura M, Nagase H, Hashimoto S, Matsuura K, Saijo S, Kobayashi T, Horii A
  • Journal Title: Cancer Sci Volume: 103(9) Pages: 1737-43
  • Peer Reviewed
• [Journal Article] Correct diagnosis of warthin tumor in the parotid gland with dynamic MRI (2012)
  • Author(s): Ogawa T, Suzuki T, Sakamoto M, Watanabe M, Tateda Y, Oshima T, Kato K, Sagai S, Kobayashi T, Shiga K
  • Journal Title: Tohoku J Exp Med Volume: 227(1) Pages: 53-7
  • NAID: 10030613225
  • Peer Reviewed
• [Journal Article] Malignant melanoma of the head and neck : A multi-institutional retrospective analysis of cases in Northern Japan (2012)
  • Author(s): Shiga K, Ogawa T, Kobayashi T, Ueda S, Kondo A, Nanba A, Kuwashima S, Asada Y, Suzuki S, Nagahashi T, Takahashi M, Suzuki M, Ishida A, Watanabe K, Harabuchi Y, Himi T, Sinkawa H, Sato H, Saijo S, Fukuda S, Tanaka K, Ishikawa K, Omori K, Aoyagi M, Hashimoto S
  • Journal Title: Head Neck Volume: 34(11) Pages: 1537-41
  • Peer Reviewed
• [Journal Article] Methylation of death-associated protein kinase is associated with cetuximab and erlotinib resistance (2012)
  • Author(s): Ogawa T, Liggett TE, Melnikov AA, Monitto CL, Kusuke D, Shiga K, Kobayashi T, Horii A, Chatterjee A, Levenson VV, Koch WM, Sidransky D, Chang X
  • Journal Title: Cell Cycle Volume: 11(8) Pages: 1656-63
  • Peer Reviewed
• [Journal Article] Methylation of death-associated protein kinase is associated with cetuximab and erlotinib resistance. (2012)
  • Author(s): Ogawa T, Liggett TE, Melnikov AA, Monitto CL, Kusuke D, Shiga K, Kobayashi T, Horii A, Chatterjee A, Levenson VV, Koch WM, Sidransky D, Chang X.
  • Journal Title: Cell Cycle. Volume: 11(8) Pages: 1656-63
  • Peer Reviewed
• [Journal Article] miR-34a is downregulated in cis-diamminedichloroplatinum treated sinonasal squamous cell carcinoma patients with poor prognosis. (2012)
  • Author(s): Ogawa T, Saiki Y, Shiga K, Chen N, Fukushige S, Sunamura M, Nagase H, Hashimoto S, Matsuura K, Saijo S, Kobayashi T, Horii A.
  • Journal Title: Cancer Sci. Volume: 103(9) Pages: 1737-43
  • Peer Reviewed
• [Journal Article] Correct diagnosis of warthin tumor in the parotid gland with dynamic MRI. (2012)
  • Author(s): Ogawa T, Suzuki T, Sakamoto M, Watanabe M, Tateda Y, Oshima T, Kato K, Sagai S, Kobayashi T, Shiga K.
  • Journal Title: Tohoku J Exp Med. Volume: 227(1) Pages: 53-7
  • NAID: 10030613225
  • Peer Reviewed
• [Journal Article] Malignant melanoma of the head and neck: A multi-institutional retrospective analysis of cases in Northern Japan. (2012)
  • Author(s): Shiga K, Ogawa T, Kobayashi T, Ueda S, Kondo A, Nanba A, Kuwashima S, Asada Y, et al.
  • Journal Title: Head Neck. Volume: 34(11) Pages: 1537-41
  • Peer Reviewed