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Functional characterization of ESRP1 and 2 in HNSCC.

Research Project

Project/Area Number 24791764
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Otorhinolaryngology
Research InstitutionUniversity of Yamanashi

Principal Investigator

ISHII Hiroki  山梨大学, 総合研究部, 助教 (40568250)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
KeywordsESRP1/2 / EMT / RNA splicing / Rac1b / dEF1/SIP1 / ESRP1 / ESRP2 / 頭頸部癌 / 選択的RNAスプライシング / EMT関連転写因子 / CD44
Outline of Final Research Achievements

ESRP1 (epithelial splicing regulatory protein 1) and ESRP2 regulate alternative splicing events associated with EMT, and these proteins are down-regulated during EMT. We examined that expression of both ESRP1 and ESRP2 is plastic. During HNSCC carcinogenesis,both are up-regulated relative to their levels in normal epithelium but down-regulated in invasive fronts into stroma. In HNSCC cell lines, ESRP1 and ESRP2 suppress cell motility of HNSCC via distinct mechanisms: knockdown of ESRP1 affects the dynamics of the actin cytoskeleton via inducing Rac1b, whereas knockdown of ESRP2 represses cell-cell adhesion through increased expression of EMT-associated transcription factors. Down-regulation of ESRP1 and ESRP2 is thus closely associated with a motile phenotype of cancer cells

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results)

  • [Journal Article] Epithelial Splicing Regulatory Protein 1 (ESRP1) and 2 (ESRP2) Suppress Cancer Cell Motility via Different Mechanisms2014

    • Author(s)
      Hiroki Ishii, Masao Saitoh, Kei Sakamoto, Tetsuo Kondo, Ryohei Katoh, Shota Tanaka, Mitsuyoshi Motizuki, Keisuke Masuyama, Keiji Miyazawa
    • Journal Title

      The journal of biological chemistry

      Volume: 289(40) Issue: 40 Pages: 27386-99

    • DOI

      10.1074/jbc.m114.589432

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ESRP1,ESRP2 は異なる機序でがん細胞の運動能を制御する2014

    • Author(s)
      石井裕貴、齋藤正夫、増山敬祐、宮澤恵二
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県 横浜市)
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] DOWN-REGULATION OF ESRP1 TRIGGERS CELL INVASION THROUGH DE-REPRESSION OF RAC1B IN HNSCC2014

    • Author(s)
      Hiroki Ishii, Keisuke Masuyama
    • Organizer
      5th IFHNOS World Congress 2014
    • Place of Presentation
      ニューヨーク(アメリカ)
    • Year and Date
      2014-07-27 – 2014-07-30
    • Related Report
      2014 Annual Research Report
  • [Presentation] ESRP1は腫瘍特異的RAC1bを抑制し、がん細胞の運動能を制御する2014

    • Author(s)
      石井裕貴、齋藤正夫、宮澤恵二
    • Organizer
      第18回日本がん分子標的治療学会
    • Place of Presentation
      仙台市産業・情報プラザ(宮城県 仙台市)
    • Year and Date
      2014-06-24 – 2014-06-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] ESRP1は腫瘍特異的Rac1bを抑制し、がん細胞の運動能を制御する2014

    • Author(s)
      石井裕貴
    • Organizer
      第18回日本がん分子標的治療学会学術集会
    • Place of Presentation
      仙台市情報・産業プラザ セミナールーム2 (宮城県仙台市)
    • Related Report
      2013 Research-status Report
  • [Presentation] DOWN-REGULATION OF ESRP1 TRIGGERS CELL INVASION THROUGH DE-REPRESSION OF RAC1B IN HNSCC2014

    • Author(s)
      Hiroki Ishii
    • Organizer
      IFHNOS 2014 5th World Congress & AHNS Annual Meeting
    • Place of Presentation
      the Marriott Marquis Hotel in Times Square in New York (米国)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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