| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
ESRP1 (epithelial splicing regulatory protein 1) and ESRP2 regulate alternative splicing events associated with EMT, and these proteins are down-regulated during EMT. We examined that expression of both ESRP1 and ESRP2 is plastic. During HNSCC carcinogenesis,both are up-regulated relative to their levels in normal epithelium but down-regulated in invasive fronts into stroma. In HNSCC cell lines, ESRP1 and ESRP2 suppress cell motility of HNSCC via distinct mechanisms: knockdown of ESRP1 affects the dynamics of the actin cytoskeleton via inducing Rac1b, whereas knockdown of ESRP2 represses cell-cell adhesion through increased expression of EMT-associated transcription factors. Down-regulation of ESRP1 and ESRP2 is thus closely associated with a motile phenotype of cancer cells
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