| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
We obtained cell lines of head and neck squamous carcinomas (FaDu, Detroit562, BICR6,BICR18). They were cultured in DMEM supplemented with 10% FBS and 1% penicillin-streptomycin. We obtained reagents and instruments to perform proliferation assay, DC protein assay, immunocytochemistry stain and flow cytometory. Proliferation assay was performed. Cells were plated in 96-well plates (1,000 cells per well) were grown in their respective media for 24, 48, or 72 hours. FaDu proliferated faster than the other cell lines. DC protein assay was performed to detect the concentration of the protein in lysate of the cell lines. We performed SDS-PAGE and Western blot analysis. Western blotting revealed that FaDu, Detroit562 and BICR6 had high expression of Galectin-3.
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