Inner ear therapy with protein transduction using polyarginine
| Project/Area Number |
24791788
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Otorhinolaryngology
|
|---|
| Research Institution | Kumamoto University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | Polyarginine / protein / inner ear / ポリアルギニン / 正円窓 / モルモット / 内耳 / protein transduction |
|---|
| Outline of Final Research Achievements |
Cell-penetrating peptides (CPPs) are short sequences of amino acids that facilitate the penetration of conjugated cargos across mammalian cell membranes. We showed that a peptide consisting of nine arginines (‘9R’) effectively delivered enhanced green fluorescent protein (EGFP) into guinea pig cochleae via the round window niche without causing any deterioration in auditory or vestibular function. A second application 24 h after the first prolonged the presence of EGFP. To assess the feasibility of protein transduction using 9R-CPPs via the round window, we used ‘X-linked inhibitor of apoptosis protein’ attached to a 9R peptide (XIAP-9R). XIAP-9R treatment prior to acoustic trauma significantly reduced putative hearing loss and the number of apoptotic hair cells in the cochleae.
|
Report
(4 results)
Research Products
(6 results)
