| Project/Area Number |
24791816
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUZUKI Hideaki 産業医科大学, 医学部耳鼻咽喉科・頭頸部外科学, 教授 (20187751)
UETA Yoichi 産業医科大学, 医学部第一生理学, 教授 (10232745)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 鼻科生理学 / pannexin channel |
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| Research Abstract |
Pannexins are a family of transmembrane nonselective channel proteins that participate in the release of ATP into extracellular space. In the present study, we investigate the expressions of pannexin-1 and purinergic P2X7 receptor in human nasal mucosa, together with hypotonic stress-induced ATP release from this tissue.
Substantial expressions of both proteins were observed in human nasal mucosa by fluorescence immunohistochemistry. The presence of the transcripts of pannexin-1 and P2X7 was also shown by qRT-PCR. Carbenoxolone (a pannexin-1 channel blocker) significantly inhibited the ATP release from the nasal mucosa, but flufenamic acid (a connexin channel blocker) and gadolinium (a stretch-activated channel blocker) did not.
These results indicate the coexistence of Panx1 and P2X7 in, and pannexin-1-dependent ATP release from, the human nasal mucosa, suggesting the possible participation of these molecules in the physiological functions of the upper airway.
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