Regulation of choroidal angiogenesis by alphaB-crystallin

• Project/Area Number: 24791822
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: KASE Satoru 北海道大学, 医学(系)研究科(研究院), 客員研究員 (60374394)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: alpha-crystallin / VEGF / 新生血管 / alphaB-crystallin / 血管新生

Research Abstract

It was hard to collect human choroidal neovascular membranes. Instead, we have isolated human epiretinal proliferative membranes in patients with diabetic retinopathy during vitrectomy. Using these samples, we analyzed expression of molecular chaperone alphaB-crystallin, a VEGF chaperone, and its phosphorylations. Indeed, alphaB-crystallin was expressed in the neovessels of the epiretinal membrane. Moreover, co-locatiozation with alphaB-crystallin and VEGF, as well as association between alphaB-crystallin serine phosphorylation and VEGF were confirmed.

Research Products

• [Journal Article] Expression of vascular endothelial growth factor in eyes with Coats disease. (2013)
  • Author(s): Kase S, Rao NA, Yoshikawa H, Fukuhara J, Noda K, Kanda A, Ishida S.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54 Issue: 1 Pages: 57-62
  • DOI: 10.1167/iovs.12-10613
  • Peer Reviewed
• [Journal Article] Expression of alphaB-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma (2013)
  • Author(s): Dong Z, Kase S, Ando R, Fukuhara J, Kinoshita S, Dong Y, Takashina S, Kanda A, Noda M, Noda K, Ishida S
  • Journal Title: Anticancer Res Volume: Vol. 33 Pages: 3745-3751
  • Peer Reviewed
• [Journal Article] Expression of vascular endothelial growth factor C in human pterygium. (2013)
  • Author(s): Fukuhara J, Kase S, Ohashi T, Ando R, Dong Z, Noda K, Ohguchi T, Kanda A, Ishida S.
  • Journal Title: Histochem Cell Biol Volume: 139 Issue: 2 Pages: 381-389
  • DOI: 10.1007/s00418-012-1019-z
  • NAID: 120005228265
  • Peer Reviewed
• [Journal Article] Expression of vascular endothelial growth factor (VEGF) and intravitreal anti-VEGF therapy with bevacizumab in vasoproliferative retinal tumors (2013)
  • Author(s): Saito W, Kase S, Fujiya A, Dong Z, Noda K, Ishida S
  • Journal Title: Retina Volume: Vol. 33 Pages: 1959-1967
  • Peer Reviewed
• [Journal Article] Autoimmune retinopathy associated with colonic adenoma. (2013)
  • Author(s): Saito W, Kase S, Ohguro H, Ishida S
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 251 Issue: 5 Pages: 1447-1449
  • DOI: 10.1007/s00417-012-2131-4
  • NAID: 120005425349
  • Peer Reviewed
• [Journal Article] TNF-α decreases VEGF secretion in highly polarized RPE cells but increases it in non-polarized RPE cells related to crosstalk between JNK and NF-k;B pathways. (2013)
  • Author(s): Terasaki H, Kase S, Shirasawa M, Otsuka H, Hisatomi T, Sonoda S, Ishida S, Ishibashi T, Sakamoto T.
  • Journal Title: PLoS One Volume: 8(7) Issue: 7 Pages: 2013-2013
  • DOI: 10.1371/journal.pone.0069994
  • Peer Reviewed
• [Journal Article] Expression of alphaB-crystallin and vascular endothelial growth factor in conjunctival squamous cell carcinoma. (2013)
  • Author(s): Dong Z, Kase S, Ando R, Fukuhara J, Kinoshita S, Dong Y, Takashina S, Kanda A, Noda M, Noda K, Ishida S.
  • Journal Title: Anticancer Res. Volume: 33 Pages: 3745-3751
  • Peer Reviewed
• [Journal Article] Expression of vascular endothelial growth factor (VEGF) and intravitreal anti-VEGF therapy with bevacizumab in vasoproliferative retinal tumors. (2013)
  • Author(s): Saito W, Kase S, Fujiya A, Dong Z, Noda K, Ishida S.
  • Journal Title: Retina Volume: 33 Issue: 9 Pages: 1959-1967
  • DOI: 10.1097/iae.0b013e3182923490
  • Peer Reviewed
• [Presentation] Induction of heat shock protein 70 ameliorates ultraviolet-induced photokeratitis in mice (2013)
  • Author(s): Kitaichi N, Lennikov A, Kase S, Noda K, Horie Y, Ohno S, Ishida S
  • Organizer: ARVO (Association for Research in Vision and Ophthalmology) Annual Meeting
  • Place of Presentation: Seattle, USA
• [Presentation] 小児結膜母斑4 症例の臨床病理学的検討 (2013)
  • Author(s): 石嶋漢, 加瀬諭, 山本哲平, 野崎真世, 石田晋
  • Organizer: 第31回 日本眼腫瘍学会, 2013年高知市総合あんしんセンター
  • Place of Presentation: 高知市
• [Presentation] 眼付属器粘液腫の臨床病理学的検討 (2013)
  • Author(s): 加瀬諭, 吉川洋, 鈴木康夫, 中村泰久, 野田実香, 石田晋
  • Organizer: 第31回日本眼腫瘍学会, 2013年高知市総合あんしんセンター
  • Place of Presentation: 高知市
• [Presentation] 両眼に多発性脈絡膜腫瘤を呈したNK 細胞リンパ腫の一例 (2013)
  • Author(s): 田川義晃, 南場研一, 水内一臣, 宇野友絵, 加瀬諭, 福原崇子
  • Organizer: 第31回日本眼腫瘍学会, 2013年高知市総合あんしんセンター
  • Place of Presentation: 高知市
• [Presentation] 血管新生緑内障を合併した眼内悪性リンパ腫の2 例 (2013)
  • Author(s): 福原淳一, 加瀬 諭, 野田実香, 南場研一, 畑中佳奈子, 石田 晋
  • Organizer: 第31回日本眼腫瘍学会, 2013年高知市総合あんしんセンター
  • Place of Presentation: 高知市
• [Presentation] Induction of heat shock protein 70 ameliorates ultraviolet-induced photokeratitis in mice (2013)
  • Author(s): Kitaichi N, Lennikov A, Kase S, Noda K, Horie Y, Ohno S, Ishida S
  • Organizer: ARVO (Association for Research in Vision and Ophthalmology) Annual Meeting
  • Place of Presentation: Washington State Convention Center (Seattle, USA)
• [Presentation] 小児結膜母斑4症例の臨床病理学的検討 (2013)
  • Author(s): 石嶋 漢、加瀬 諭、山本 哲平、野崎 真世、石田 晋
  • Organizer: 第31回 日本眼腫瘍学会
  • Place of Presentation: 高知市総合あんしんセンター（高知市）
• [Presentation] 眼付属器粘液腫の臨床病理学的検討 (2013)
  • Author(s): 加瀬 諭、吉川 洋、鈴木 康夫、中村 泰久、野田実香、石田 晋.
  • Organizer: 第31回 日本眼腫瘍学会
  • Place of Presentation: 高知市総合あんしんセンター（高知市）
• [Presentation] 両眼に多発性脈絡膜腫瘤を呈したNK細胞リンパ腫の一例 (2013)
  • Author(s): 田川 義晃、南場 研一、水内 一臣、宇野 友絵、加瀬 諭、福原 崇子 齋藤 航 、石田 晋.
  • Organizer: 第31回 日本眼腫瘍学会
  • Place of Presentation: 高知市総合あんしんセンター（高知市）
• [Presentation] 血管新生緑内障を合併した眼内悪性リンパ腫の2例 (2013)
  • Author(s): 福原淳一、加瀬 諭、野田実香、南場研一、畑中佳奈子、石田 晋
  • Organizer: 第31回 日本眼腫瘍学会
  • Place of Presentation: 高知市総合あんしんセンター（高知市）