Efficacy of naturally-derived anti-oxidant pigments on uveitis model

• Project/Area Number: 24791826
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Hokkaido University
• Principal Investigator: MIZUUCHI Kazuomi 北海道大学, 大学病院, 医員 (80598780)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エキノクローム / ぶどう膜炎 / 天然由来色素 / フコキサンチン

Outline of Final Research Achievements

Lewis rats were treated with 0.1, 1.0, or 10mg/kg of echinochrome systemically. Then, lipopolysaccharide (LPS) was injected into rats to induce endotoxin-induced uveitis (EIU). The numbers of aqueous cells, protein levels, and TNF-alpha concentrations were significantly decreased in echinochrome-treated rats compared with untreated rats in 24 hours. It was in a dose-dependent decrease.
Immunohistochemical examination showed that reactive oxygen species (ROS) and NF-kappaB signals were decreased in iris/ciliary body.

Research Products

• [Journal Article] Development and validation of new diagnostic criteria for acute retinal necrosis. (2015)
  • Author(s): Takase H, Okada AA, Goto H, Mizuki N, Namba K, Ohguro N, Sonoda KH, Tomita M, Keino H, Kezuka T, Kubono R, Mizuuchi K, Shibuya E, Takahashi H, Yanai R, Mochizuki M
  • Journal Title: Jpn J Ophthalmol Volume: 59(1) Issue: 1 Pages: 14-20
  • DOI: 10.1007/s10384-014-0362-0
  • NAID: 210000165197
  • Peer Reviewed / Open Access
• [Journal Article] Amelioration of experimental autoimmune uveoretinitis by inhibition of glyceraldehyde derived-advanced glycation end product formation. (2014)
  • Author(s): Dong Z, Iwata D, Kitaichi N, Takeuchi M, Sato M, Endo N, Iwabuchi K, Ando R, Fukuhara J, Kinoshita S, Lennikov A, Kitamura M, Mizuuchi K, Kanda A, Noda K, Namba K, Yamagishi S, Ohno S, Ishida S
  • Journal Title: J Leukoc Biol Volume: 96 Issue: 6 Pages: 1077-1085
  • DOI: 10.1189/jlb.3a0513-288rrr
  • Peer Reviewed / Open Access
• [Journal Article] Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome (2014)
  • Author(s): 1.Kitaichi N, Lennikov A, Noda K, Mizuuchi K, Namba K, Ohno S, Ishida S
  • Journal Title: Mol Vis Volume: 7 Pages: 171-177
  • Peer Reviewed / Open Access
• [Journal Article] Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome in rats (2014)
  • Author(s): Lennikov A, Kitaichi N, Noda K, Mizuuchi K, Ando R, Dong Z, Fukuhara J, Kinoshita S, Namba K, Ohno S, Ishida S.
  • Journal Title: Mol Vis Volume: 20 Pages: 171-177
  • NAID: 120005427742
  • Peer Reviewed
• [Journal Article] Trabecular meshwork depigmentation in Vogt-Koyanagi-Harada disease. (2013)
  • Author(s): Mizuuchi K, Kitaichi N, Namba K, Horie Y, Ishida S, Ohno S
  • Journal Title: Jpn J Ophthalmol Volume: 57 Issue: 3 Pages: 245-251
  • DOI: 10.1007/s10384-012-0229-1
  • NAID: 10031176278
  • Peer Reviewed
• [Journal Article] Two cases of subfoveal choroidal neovascularization with tubulointerstitial nephritis and uveitis (TINU) syndrome. (2013)
  • Author(s): Takemoto Y, Namba K, Mizuuchi K, Ohno S, Ishida S.
  • Journal Title: Eur J Ophthalmol Volume: 23 Issue: 2 Pages: 255-257
  • DOI: 10.5301/ejo.5000240
  • Peer Reviewed
• [Journal Article] Correlation between elevation of serum antinuclear antibody titre and decreased therapeutic efficacy in the treatment of Behçet’s disease with infliximab. (2012)
  • Author(s): Iwata D, Namba K, Mizuuchi K, Kitaichi N, Kase S, Takemoto Y, Ohno S, Ishida S.
  • Journal Title: Graefes Arch Clin Exp Ophthalmol Volume: 250 Issue: 7 Pages: 1081-1087
  • DOI: 10.1007/s00417-011-1908-1
  • Peer Reviewed
• [Presentation] Topical rebamipide improves subjective symptoms of dry eye by modulating pain sensation in cornea (2015)
  • Author(s): Tagawa Y, Kitaichi N, Ohguchi T, Ishizuka ET, Inafuku S, Dong Y, Kinoshita S, Iwata D, Mizuuchi K, Kanda A, Noda K, Ishida S
  • Organizer: Asia-ARVO(Association for Research in Vision and Ophthalmology)2015: Yokohama, Japan
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2015-02-16 – 2015-02-19
• [Presentation] Correlation between ocular inflammatory attacks and serum trough level of infliximab or serum concentration of cytokines in the clinical course of behcet’s disease patients with infliximab treatment (2015)
  • Author(s): Mizuuchi K, Namba K, Takemoto Y, Ohno S, Ishida S
  • Organizer: Asia-ARVO(Association for Research in Vision and Ophthalmology)2015: Yokohama, Japan
  • Place of Presentation: パシフィコ横浜（横浜市）
  • Year and Date: 2015-02-16 – 2015-02-19
• [Presentation] 眼所見を中心に粘膜病変のみ呈した Stevens-Johnson症候群の1例 (2015)
  • Author(s): 木嶋理紀、大口剛司、 北尾仁奈、岩田大樹、水内一臣、野村友希子、田川義継、石田晋
  • Organizer: 角膜カンファランス2015
  • Place of Presentation: 高知市文化プラザかるぽーと（高知市）
  • Year and Date: 2015-02-11 – 2015-02-13
• [Presentation] Amelioration of endotoxin-induced uveitis treated with the sea urchin pigment echinochrome (2014)
  • Author(s): Kitaichi N, Lennikov A, Noda K, Mizuuchi K, Namba K, Ohno S, Ishida S
  • Organizer: 16th International Conference on Behcet's disease
  • Place of Presentation: Cite Internationale Universitaire de Paris （Paris, France）
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] The appearance of antibodies to infliximab in the course of infliximab administration in Behçet’s disease (2014)
  • Author(s): Namba K, Mizuuchi K, Takemoto Y, Tagawa Y, Uno T, Fukuhara T, Ohno S, Kitaichi N, Ishida S
  • Organizer: 16th International Conference on Behcet's disease
  • Place of Presentation: Cite Internationale Universitaire de Paris （Paris, France）
  • Year and Date: 2014-09-18 – 2014-09-20
• [Presentation] Correlation between ocular inflammatory attacks and serum trough level of infliximab or serum concentration of cytokines in the clinical course of behcet’s disease patients with infliximab treatment (2014)
  • Author(s): Mizuuchi K, Namba K, Takemoto Y, Ohno S, Ishida S
  • Organizer: World Ophthalmology Congress 2014 Tokyo
  • Place of Presentation: 東京国際フォーラム（東京都）
  • Year and Date: 2014-04-02 – 2014-04-04
• [Presentation] ぶどう膜炎における網羅的糖差解析 (2013)
  • Author(s): 南場研一、北市伸義、安藤 亮、竹本裕子、水内一臣、堀江幸弘、大野重昭、天野麻穂、西村紳一郎、石田 晋
  • Organizer: 第117回 日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム（東京都）
• [Presentation] ぶどう膜炎を合併した急性帯状潜在性網膜外層症の1例 (2013)
  • Author(s): 宇野友絵、南場研一、齋藤 航、水内一臣、福原崇子、田川義晃、石田 晋
  • Organizer: 第67回日本臨床眼科学会
  • Place of Presentation: パシフィコ横浜（横浜市）
• [Presentation] Correlation between development of antinuclear antibody titre and decreased therapeutic efficacy in the treatment of Behcet’s disease with infliximab (2012)
  • Author(s): Namba K, Iwata D, Mizuuchi K, Kitaichi N, Kase S, Takemoto Y, Ohno S, Ishida S
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: 横浜コンベンションセンター（ 横浜市）
• [Presentation] Effects of Infliximab Therapy in Behcet's disease (2012)
  • Author(s): Takemoto Y, Namba K, Kitaichi N, Mizuuchi K, Ohno S, Ishida S.
  • Organizer: 15th International Conference on Behcet's Disease
  • Place of Presentation: 横浜コンベンションセンター（ 横浜市）
• [Presentation] Vogt-小柳原田病の前眼部炎症再発時における脈絡膜循環障害 (2012)
  • Author(s): 竹本裕子、南場研一、水内一臣、橋本勇希、宇野友絵、北市伸義、大野重昭、石田 晋.
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 国立京都国際会館（ 京都府）
• [Presentation] 多発脳神経炎の治療中に急性網膜壊死を発症した1例 (2012)
  • Author(s): 水内一臣、南場研一、齋藤 航、堀内一宏、加納崇裕、竹本裕子、北市伸義、大野重昭、石田 晋.
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 国立京都国際会館（ 京都府）
• [Presentation] 隅角結節を伴う続発緑内障の臨床像 (2012)
  • Author(s): 竹本裕子、新田卓也、南場研一、水内一臣、宇野友絵、大口剛司、新明康弘、陳 進輝、石田 晋.
  • Organizer: 第23回緑内障学会
  • Place of Presentation: ANAクラウンプラザホテル（ 金沢市）
• [Presentation] 尋常性感癬に伴う難治性ぶどう膜炎に対しインフリキシマブ治療が奏功した1例 (2012)
  • Author(s): 櫻井友梨、南場研一、水内一臣、竹本裕子、宇野友絵、及村俊史、大野重昭、石田 晋.
  • Organizer: 第159回 北海道眼科集談会
  • Place of Presentation: 旭川ターミナルホテル（ 旭川市）
• [Presentation] 網膜色素上皮裂孔および漿液性網膜剥離を伴ったぶどう膜炎 (2012)
  • Author(s): 田川義晃、南場研一、水内一臣、竹本裕子、北市伸義、大野重昭、石田 晋.
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜（ 横浜市）
• [Presentation] Relentless placoid chorioretinitisの1例 (2012)
  • Author(s): 6. 竹本裕子、南場研一、齋藤 航、水内一臣、北市伸義、大野重昭、石田 晋.
  • Organizer: 第46回眼炎症学会
  • Place of Presentation: パシフィコ横浜（ 横浜市）
• [Presentation] Vogt-小柳―原田病における隅角色素脱失（大野徴候）の検討 (2012)
  • Author(s): 水内一臣、南場研一、北市伸義、堀江幸弘、岩田大樹、石田 晋、大野重昭.
  • Organizer: 第116回日本眼科学会
  • Place of Presentation: パシフィコ横浜（ 横浜市）
• [Presentation] ベーチェット病における眼炎症発作とインフリキシマブトラフ値およびサイトカイン血中濃度との相関 (2012)
  • Author(s): 南場研一、大野重昭、北市伸義、竹本裕子、水内一臣、宇野友絵.
  • Organizer: 厚生労働省難病克服対策事業 ベーチェット病に関する調査研究 第二回班会議
  • Place of Presentation: 横浜市立大学（ 横浜市）