Basic study for the inhibitory effects of trehalose on malignant melanoma cells

• Project/Area Number: 24791831
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Hirosaki University
• Principal Investigator: KUDO Takashi 弘前大学, 医学部附属病院, 助教 (20598718)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: トレハロース / 癌化学療法 / 外眼部 / 悪性黒色腫 / 結膜腫瘍 / 腫瘍化学療法 / 細胞周期 / アポトーシス / 国際情報交換

Research Abstract

Human cutaneous malignant melanoma cells were implanted in the subconjunctival spaces of nude rats. Various concentrations of trehalose were instilled (in vivo system) and melanoma cells were cultivated under various concentrations of trehalose for analyzing changes of proliferative activities of tumor cells by MTT assay and flow-cytometrically quantifying expressions of some kinds of transcription factors that were known to be important for cell cycle (in vitro system). Trehalose dose-dependently showed inhibition of malignant cell proliferation and through transcription factors and demonstrated to lead tumor cells to apoptosis in association with transcription factors.

Research Products

• [Journal Article] プロピルチオウラシルにより両球後視神経炎を生じた ANCA関連血管炎の1例 (2013)
  • Author(s): 工藤孝志、鈴木香、鈴木幸彦、工藤朝香、木村智美、伊藤忠、片山君予、中澤満
  • Journal Title: 臨床眼科 Volume: 67(2) Pages: 209-213
  • Peer Reviewed
• [Journal Article] Long-term effects of nilvadipine against progression of the central visual field defect in retinitis pigmentosa: An extended study (2013)
  • Author(s): Nakazawa M, Suzuki Y, Ito T, Metoki T, Kudo T, Ohguro H
  • Journal Title: Biomed Res Int 2013 Pages: 585729-585729
  • Peer Reviewed
• [Journal Article] Long-term effects of nilvadipine against progression of the central visual field defect in retinitis pigmentosa: An extended study. (2013)
  • Author(s): Nakazawa M, Suzuki Y, Ito T, Metoki T, Kudo T, Ohguro H.
  • Journal Title: BioMed Research International Volume: 2013
  • Peer Reviewed
• [Journal Article] プロピルチオウラシルにより両球後視神経炎を生じたANCA関連血管炎の1例 (2013)
  • Author(s): 工藤孝志、鈴木 香、鈴木幸彦、工藤朝香、木村智美、伊藤 忠、片山君予、中澤 満
  • Journal Title: 臨床眼科 Volume: 67 Pages: 209-213
  • Peer Reviewed
• [Journal Article] Inhibitory effects of trehalose on malignant melanoma cell growth: implications for a novel topical anti-cancer agent on the ocular surface. (2012)
  • Author(s): Kudo T, Takeuchi K, Ebina Y, Nakazawa M.
  • Journal Title: ISRN Ophthalmology Volume: 2012 Pages: 1-9
  • DOI: 10.5402/2012/968493
  • Peer Reviewed
• [Journal Article] Inhibitory effects of trehalose on malignant melanoma cell growth: implications for a novel topical anti-cancer agent on the ocular surface. (2012)
  • Author(s): Kudo T, Takeuchi K, Ebina Y, Nakazawa M
  • Journal Title: ISRN Ophthalmology Volume: 2012 Pages: 1-9
  • Peer Reviewed
• [Presentation] 9シスβカロテン高含有ドナリエラバーダウイルのRPE65-/-マウス錐体オプシン保護作用 (2013)
  • Author(s): 工藤孝志、尾崎 拓、中澤 満
  • Organizer: 第117回日本眼科学会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] 診断・治療方針に難儀した強膜・ぶどう膜炎の1例 (2012)
  • Author(s): 工藤孝志
  • Organizer: 第6回東北6大学眼科Step Upセミナー
  • Place of Presentation: 仙台
  • Year and Date: 2012-09-23
• [Presentation] 神経膠腫を疑い視神経鞘髄膜腫であることが判明した1例 (2012)
  • Author(s): 工藤孝志、木村智美、安達功武、横井由美子、宮川靖博、鈴木幸彦、中澤満、工藤香名江、伊藤勝博、大熊洋揮、黒瀬顕
  • Organizer: 第66回日本臨床眼科学会
  • Place of Presentation: 京都
• [Presentation] トレハロースによる悪性黒色腫細胞増殖抑制効果 (2012)
  • Author(s): 工藤孝志、竹内侯雄、蝦名祐一、中澤 満
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム
• [Presentation] Trehaloseによる melanoma細胞抑制効果について (2010)
  • Author(s): 工藤孝志、竹内侯雄、中澤満
  • Organizer: 第119回青森眼科集談会
  • Place of Presentation: 弘前市
  • Year and Date: 2010-10-31