Establishment of anti-choroidal neovascularization tailor-made therapy

• Project/Area Number: 24791832
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Tohoku University
• Principal Investigator: ISHIKAWA Yumi 東北大学, 医学(系)研究科(研究院), 技術補佐員 (60396439)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 眼科臨床 / 眼医工学

Outline of Final Research Achievements

For optimum VEGF antibody administration, we aimed to develop a tailor-made treatment. This purpose included the optimization of the method of administration and examination of VEGF-stimulating factor. We analyzed RPE, which was stressed by hypoxic culture and low glucose, to find possible VEGF expression enhancing factors this year. From the results of proteomic analysis, the known-gene was presumed to be important, we analyzed the factors by additional real-time PCR and Western blotting. Expression of VEGF was confirmed to be suppressed with the resulting siRNA. Since gene KO mice of this factor was found to have been made, we performed an individual preparation of the mice. We initiated photocoagulation induced neovascularization model studies currently.

Research Products

• [Journal Article] Intrascleral Transplantation of a Collagen Sheet with Cultured Brain-Derived Neurotrophic Factor Expressing Cells Partially Rescues the Retina from Damage due to Acute High Intraocular Pressure. (2014)
  • Author(s): Abe T, Tokita-Ishikawa Y, Onami H, Katsukura Y, Kaji H, Nishizawa M, Nagai N.
  • Journal Title: Adv Exp Med Biol Volume: 801 Pages: 837-843
  • DOI: 10.1007/978-1-4614-3209-8_105
  • ISBN: 9781461432081, 9781461432098
  • Peer Reviewed / Open Access
• [Journal Article] A Platform for Controlled Dual-Drug Delivery to the Retina : Protective Effects against Light-Induced Retinal Damage in Rats (2014)
  • Author(s): N Nagai, H Kaji, H Onami, Y Katsukura, Y Ishikawa, ZK Nezhad, K Sampei, S Iwata, S Ito, M Nishizawa, T Nakazawa, N Osumi, Y Mashima, T Abe
  • Journal Title: Advanced Healthcare Materials Volume: in press Issue: 10 Pages: 680-687
  • DOI: 10.1002/adhm.201400114
  • Peer Reviewed / Open Access
• [Journal Article] A polymeric device for controlled transscleral multi-drug delivery to the posterior segment of the eye (2014)
  • Author(s): Nobuhiro Nagai, Hirokazu Kaji, Hideyuki Onami, Yumi Ishikawa, Matsuhiko Nishizawa, Noriko Osumi, Toru Nakazawa, Toshiaki Abe
  • Journal Title: Acta Biomaterialia Volume: 10(2) Issue: 2 Pages: 680-6877
  • DOI: 10.1016/j.actbio.2013.11.004
  • NAID: 40020425298
  • Peer Reviewed
• [Journal Article] Reduction of laser-induced choroidal neovascularization by intravitreal vasohibin-1 in monkey eyes (2012)
  • Author(s): Hideyuki Onami, Nobuhiro Nagai, Shigeki Machida, Norihiro Kumasaka, Ryosuke Wakusawa, Yumi Ishikawa, Hikaru Sonoda, Yasufumi Sato, Toshiaki Abe.
  • Journal Title: RETINA The Journal of Retinal and Vitreous Diseases Volume: 32 Issue: 6 Pages: 1204-1213
  • DOI: 10.1097/iae.0b013e318233ad0b
  • Peer Reviewed
• [Journal Article] Vasohibin-1 and retinal pigment epithelium (2012)
  • Author(s): Yumi Ishikawa, Nobuhiro Nagai, Hideyuki Onami, Norihiro Kumasaka, Ryosuke Wakusawa, Hikaru Sonoda, Yasufumi Sato, Toshiaki Abe
  • Journal Title: Adv Exp Med Biol Volume: 723 Pages: 305-310
  • DOI: 10.1007/978-1-4614-0631-0_40
  • ISBN: 9781461406303, 9781461406310
  • Peer Reviewed
• [Presentation] Protective Effects of Transscleral Drug Delivery Device Against Photoreceptor Cell Death in S334ter Rhodopsin Mutant Rats (2013)
  • Author(s): Nagai N, Kaji H, Onami H, Yamada T, Katsukura Y, Ishikawa Y, Nishizawa M, Mashima Y, Abe T.
  • Organizer: ARVO annual meeting
  • Place of Presentation: Seattle, Washington,USA
• [Presentation] Suppression of Rat Choroidal Neovascularization by Transscleral Vasohibin-1 Delivery Device (2012)
  • Author(s): 3. Hideyuki Onami, Nobuhiro Nagai, Ryosuke Wakusawa , Hirokazu Kaji, Takuya Yamada, Yumi Ishikawa, Matsuhiko Nishizawa, Yasufumi Sato, Toru Nakazawa,and Toshiaki Abe
  • Organizer: 2012 ARVO annual meeting
  • Place of Presentation: Fort Lauderdale, US
• [Presentation] Intra-scleral transplantation of collagen sheet with cultured brain-derived neurotrophic factor expressing cells partially rescued the retina from the damage of acute high intraocular pressure (2012)
  • Author(s): 1. Toshiaki Abe, Yumi Ishikawa, Hideyuki Onami, Yuki Katsukura, Nobuhiro Nagai
  • Organizer: RD2012 XV International Symposium on Retinal Degeneration
  • Place of Presentation: Bavaria, Germany
• [Presentation] Protective Effects of Transscleral Drug Delivery Device Against Light-induced Retinal Damage in Rats (2012)
  • Author(s): 2. Nobuhiro Nagai, Hideyuki Onami, Hirokazu Kaji, Takuya Yamada, Yuki Katsukura, Machiko Sato, Yumi Ishikawa, Toru Nakazawa, Matsuhiko Nishizawa, and Toshiaki Abe
  • Organizer: 2012 ARVO annual meeting
  • Place of Presentation: Fort Lauderdale, US