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Molecular functional analysis of the modified volvox-derived channelrhodopsin-1

Research Project

Project/Area Number 24791833
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Ophthalmology
Research InstitutionIwate University

Principal Investigator

MURAYAMA Namie  岩手大学, 工学部, 学術研究員 (60597516)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords眼生理 / パッチクランプ / 眼生理学
Research Abstract

The sensitive wavelength of channelrhodopsin2 (ChR2) derived from chlamydomonas is limited under 540 nm corresoponding to blue color. On the other hands, it has been reported that the volvox derived channelrhodopsin-1 (VChR1) had a red sifted sensitivities compared to ChR2. We developed a new type of channelrhodopsin which had a broad band wavelength sensitivities by modifying the VChR1 and investigated the ion channel properties.
After establishing the cell line which expressed mVChR1 gene (HEK-mVChR1), we measuredthe photo current caused by a light stimuli. The photo current in the modified VChR1-expressing cell induced by a stimuli of blue, green, yellow and red light, which showed approximately 30 timeshigher currents compared to that of the native VChR1. In consideration to human visual spectrum, mVChR1 was useful for the purpose of restoring vision

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (20 results)

All 2014 2013 2012 Other

All Journal Article (6 results) (of which Peer Reviewed: 5 results) Presentation (14 results) (of which Invited: 3 results)

  • [Journal Article] Restoration of the majority of the visual spectrum by using modified Volvox channelrhodopsin-12014

    • Author(s)
      Tomita H, Sugano E, Murayama N, Ozaki T, Nishiyama F, Tabata K, Takahashi M, Saito T, Tamai M
    • Journal Title

      Mol Ther

      Volume: (in press)

    • NAID

      120007179833

    • Related Report
      2013 Final Research Report
  • [Journal Article] Essential role of thioredoxin 2 in mitigating oxidative stress in retinal epithelial cells.2013

    • Author(s)
      Sugano E, Murayama N, Takahashi M, Tabata K, Tamai M, Tomita H.
    • Journal Title

      J Ophthalmol.

      Volume: 2013 Pages: 185825-185825

    • DOI

      10.1155/2013/185825

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different anti-oxidant effects of thioredoxin 1 and thioredoxin 2 in retinal epithelial cells2013

    • Author(s)
      Sugano, E. Isago, H. Murayama, N. Tamai, M. Tomita, H
    • Journal Title

      Cell Struct Funct

      Volume: 38(1)巻 Pages: 81-88

    • NAID

      130004053892

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Establishment of monocular-limited photoreceptor degeneration models in rabbits.2013

    • Author(s)
      Isago H, Sugano E, Murayama N, Tamai M, Tomita H.
    • Journal Title

      BMC Ophthalmol.

      Volume: 13 Issue: 1 Pages: 19-19

    • DOI

      10.1186/1471-2415-13-19

    • NAID

      120005550125

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Different anti-oxidant effects of thioredoxin 1 and thioredoxin 2 in retinal epithelial cells2013

    • Author(s)
      Sugano, E. Isago, H. Murayama, N. Tamai, M.
    • Journal Title

      Cell Struct Funct

      Volume: 38(1) Pages: 81-88

    • NAID

      130004053892

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different anti-oxidant effects of thioredoxin 1 and thioredoxin 2 in retinal epithelial cells.2013

    • Author(s)
      Sugano E,Isago H,Murayama N,Tamai M,Tomita H
    • Journal Title

      Cell Structure and Function

      Volume: 38 Pages: 81-88

    • NAID

      130004053892

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 網膜から脳へのシグナル : 光による修飾, 行動との相関, 視覚の再建 「オプトジェネティクスの視覚への応用-失明者の視覚再建に向けて-」2014

    • Author(s)
      冨田浩史, 菅野江里子, 村山奈美枝, 田端希多子, 高橋麻紀, 斎藤建彦, 西山史朗, 玉井信
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学 郡元キャンパス
    • Year and Date
      2014-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 光計測と操作による先端脳研究「オプトジェネティクスの視覚への応用」2013

    • Author(s)
      冨田浩史, 菅野江里子, 村山奈美枝, 田端希多子, 高橋麻紀, 斎藤建彦, 西山史朗, 玉井信
    • Organizer
      第13回生理学若手サマースクール
    • Place of Presentation
      順天堂大学センチュリータワー南13 階
    • Year and Date
      2013-08-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 視機能再建のための遺伝子治療・現状と課題2013

    • Author(s)
      冨田浩史, 菅野江里子, 村山奈美枝, 田端希多子, 高橋麻紀, 斎藤建彦, 西山史朗, 玉井信
    • Organizer
      日本網膜色素変性症協会(JRPS)長野県支部医療講演会
    • Place of Presentation
      長野県佐久平野沢会館
    • Year and Date
      2013-06-23
    • Related Report
      2013 Final Research Report
  • [Presentation] カニクイザルの片眼視細胞変性モデルの確立2012

    • Author(s)
      砂金ひとみ, 菅野江里子, 村山奈美枝, 原富雄, 萩森一郎, 玉井信, 冨田浩史
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学豊中キャンパス
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 変異型チャネルロドプシンのパッチクランプ法による評価2012

    • Author(s)
      村山奈美枝, 菅野江里子, 砂金ひとみ, 斎藤建彦, 玉井信, 冨田浩史
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪大学豊中キャンパス
    • Year and Date
      2012-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] Establishment of a method for the visual acuity test on cynomolgus monkey2012

    • Author(s)
      Hiroshi Tomita, Hitomi Isago, Eiji Iwata, Namie Murayama, Yuri Shinomoto, Masami Watanabe, Makoto Tamai, Eriko Sugano
    • Organizer
      The Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fortlauderdale, FL
    • Year and Date
      2012-05-09
    • Related Report
      2013 Final Research Report
  • [Presentation] Restore vision covering visible light by using single gene, modified volvox channelrhodopsin-12012

    • Author(s)
      Eriko Sugano, Hitomi Isago, Namie Murayama, Takehiko Saito, Yuri Shinomoto, Makoto Tamai and Hiroshi Tomita
    • Organizer
      The Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fortlauderdale, FL
    • Year and Date
      2012-05-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 視機能再建のための遺伝子治療・現状と課題

    • Author(s)
      冨田浩史、菅野江里子、村山奈美枝、田端希多子、高橋麻紀、斎藤建彦、西山史朗、玉井信
    • Organizer
      日本網膜色素変性症協会(JRPS)長野県支部医療講演会
    • Place of Presentation
      長野県 佐久平 野沢会館
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 光計測と操作による先端脳研究 「オプトジェネティクスの視覚への応用」

    • Author(s)
      冨田浩史、菅野江里子、村山奈美枝、田端希多子、高橋麻紀、斎藤建彦、西山史朗、玉井信
    • Organizer
      第13回生理学若手サマースクール
    • Place of Presentation
      順天堂大学センチュリータワー
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 網膜から脳へのシグナル:光による修飾、行動との相関、視覚の再建 「オプトジェネティクスの視覚への応用-失明者の視覚再建に向けて-」

    • Author(s)
      冨田浩史、菅野江里子、村山奈美枝、田端希多子、高橋麻紀、斎藤建彦、西山史朗、玉井信
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学 郡元キャンパス
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Restore vision covering visible light by using single gene, modified volvox channelrhodopsin-1

    • Author(s)
      Sugano E, Isago H, Murayama N, Saitoh T, Tamai M, Tomita H
    • Organizer
      Annual meeting Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Ft.Lauderdal,FL.(アメリカ)
    • Related Report
      2012 Research-status Report
  • [Presentation] Establishment of a method for the visual acuity test on cynomolgus monkey

    • Author(s)
      Tomita H ,Isago H, Iwata E, Murayama N, Shinomoto Y, Watanabe M, Tamai M, Sugano E
    • Organizer
      Annual meeting Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Ft.Lauderdal,FL.(アメリカ)
    • Related Report
      2012 Research-status Report
  • [Presentation] 変異型チャネルロドプシンのパッチクランプ法による評価

    • Author(s)
      村山奈美枝,菅野江里子,砂金ひとみ,斎藤建彦,玉井信,富田浩史
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪大学豊中キャンパス(大阪)
    • Related Report
      2012 Research-status Report
  • [Presentation] カニクイザルの片眼視細胞変性モデルの確立

    • Author(s)
      砂金ひとみ,菅野江里子,村山奈美枝,原富雄,萩森一郎,玉井信,富田浩史
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪大学豊中キャンパス(大阪)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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