Molecular functional analysis of the modified volvox-derived channelrhodopsin-1
| Project/Area Number |
24791833
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | Iwate University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | 眼生理 / パッチクランプ / 眼生理学 |
|---|
| Research Abstract |
The sensitive wavelength of channelrhodopsin2 (ChR2) derived from chlamydomonas is limited under 540 nm corresoponding to blue color. On the other hands, it has been reported that the volvox derived channelrhodopsin-1 (VChR1) had a red sifted sensitivities compared to ChR2. We developed a new type of channelrhodopsin which had a broad band wavelength sensitivities by modifying the VChR1 and investigated the ion channel properties.
After establishing the cell line which expressed mVChR1 gene (HEK-mVChR1), we measuredthe photo current caused by a light stimuli. The photo current in the modified VChR1-expressing cell induced by a stimuli of blue, green, yellow and red light, which showed approximately 30 timeshigher currents compared to that of the native VChR1. In consideration to human visual spectrum, mVChR1 was useful for the purpose of restoring vision
|
Report
(3 results)
Research Products
(20 results)
-
-
-
-
-
-
-
-
-
[Presentation] 視機能再建のための遺伝子治療・現状と課題2013
Author(s)
冨田浩史, 菅野江里子, 村山奈美枝, 田端希多子, 高橋麻紀, 斎藤建彦, 西山史朗, 玉井信
Organizer
日本網膜色素変性症協会(JRPS)長野県支部医療講演会
Place of Presentation
長野県佐久平野沢会館
Year and Date
2013-06-23
Related Report
-
-
-
-
-
-
-
-
-
-
-
