The role of the Nrf2-mediated defense system in stem cell niche
| Project/Area Number |
24791851
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Ophthalmology
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| Research Institution | Osaka University |
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Principal Investigator |
HAYASHI Ryuhei 大阪大学, 医学(系)研究科(研究院), 助教 (70535278)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | Nrf2 / 角膜上皮 / 角膜上皮幹細胞 / 創傷治癒 / 細胞遊走 / 細胞増殖 / 幹細胞ニッチ / Ｎｒｆ２ |
|---|
| Research Abstract |
The purpose of this study is to clarify the involvement of Nrf2-mediated defense system in corneal epithelial regeneration. Results of corneal epithelial wound-healing experiments demonstrated that corneal epithelial wound healing in Nrf2 KO mice were significantly delayed in 24-48 hours after injury, compared to that in WT. To clarify the role of Nrf2 during wound healing, we performed in vitro experiments with siRNA for Nrf2 and its suppressor Keap1. Nrf2 knockdown significantly delayed corneal epithelial cell migration, but did not affect cell proliferation. Conversely, Keap1 knockdown significantly accelerated cell migration. These results suggest that Nrf2 contributed to the corneal epithelial wound-healing process by accelerating cell migration.
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Report
(3 results)
Research Products
(42 results)
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[Journal Article] Cell transportation technique by airplane for clinical trial using tissue-engineered human oral mucosal epithelial cell sheets2013
Author(s)
Oie Y , Nozaki T , T akayangi H, Hara S, Hayashi R, T akeda S, Mori K, Moriya N, Soma T , T sujikawa M, Saito K, and Nishida K
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Journal Title
Tissue Engineer ing part A
Volume: (in press)
Related Report
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[Journal Article] The Role of Nrf2-Mediated Defense System in Corneal Epithelial Wound Healing2013
Author(s)
Hayashi R, Himori N, Taguchi K, Ishikawa Y, Uesugi K, Ito M, Yamamoto M, Thomas D, Tsujikawa M, Nakazawa T, Yamamoto M, Nishida K
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Journal Title
Free Radic Biol Med
Volume: 61C
Pages: 333-342
Related Report
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[Journal Article] Validation of Na, K-A Tpase pump function in corneal endothelial cells for corneal regenerative medicine2013
Author(s)
Hato S, Higa K, Inagaki E, Y oshida S, Kimura E, Hayashi R, T sujikawa M, T subota K, Nishida K, Shimmura S
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Journal Title
Tissue Engineering part A
Volume: (in press)
Related Report
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[Journal Article] Validation of Na,K-ATPase pump function in corneal endothelial cells for corneal regenerative medicine.2013
Author(s)
Hato S, Higa K, Inagaki E,Yoshida S, Kimura E, Hayashi R, Tsujikawa M, Tsubota K, Nishida K, Shimmura S.
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Journal Title
Tissue Engineering part A
Volume: in press
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Peer Reviewed
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[Presentation]2013
Author(s)
Susumu Hara, Ryuhei Hayashi, Thomas Duncan, Motokazu T sujikawa, T oru Nakazawa, Masayuki Y amamoto, Kohji Nishida
Organizer
ARVO 2013
Place of Presentation
Seattle WA
Year and Date
2013-05-05
Related Report
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[Presentation] The Role of Nrf2-Mediated Defense System in Corneal Epithelial Wound Healing2013
Author(s)
Ryuhei Hayashi, Noriko Himori, Keiko T aguchi, Y uki Ishikawa, Kohji Uesugi, Miyuki Ito, Thomas Duncan, Motokazu T sujikawa, T oru Nakazawa, Masayuki Y amamoto, Kohji Nishida
Organizer
ARVO 2013
Place of Presentation
Seattle WA
Year and Date
2013-05-05
Related Report
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