Investigation of the roles of Mrp4 in retinal angiogenesis

• Project/Area Number: 24791854
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kobe University
• Principal Investigator: KUSUHARA SENTARO 神戸大学, 医学(系)研究科(研究院), 助教 (40437463)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Ｍｒｐ４ / ＡＢＣトランスポーター / 網膜 / 血管新生 / 酸素誘発網膜症モデル / MRP4 / 周皮細胞 / cAMP

Outline of Final Research Achievements

This study showed Mrp4, an efflux transporter, is intricately associated with physiological retinal angiogenesis by regulating intracellular cAMP level. Additionally, as both the formation of avascular area and extraretinal neovascularization were exacerbated in Mrp4 knockout mice in an oxygen-induced retinopathy model, Mrp4 seems to have a suppressive role in pathological retinal angiogenesis. These results suggest that Mrp4 is a promising therapeutic target for neovascular retinal diseases.

Research Products

• [Journal Article] Forskolin modifies retinal vascular development in Mrp4-knockout mice (2012)
  • Author(s): Matsumiya W, Kusuhara S, Hayashibe K, Maruyama K, Kusuhara H, Tagami M, Schuetz JD, Negi A.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 53 Issue: 13 Pages: 8029-8035
  • DOI: 10.1167/iovs.12-10781
  • Peer Reviewed
• [Presentation] The role of Mrp4 in pathological angiogenesis of the mouse retina (2015)
  • Author(s): Sentaro Kusuhara, Wataru Matsumiya, Makoto Nakamura
  • Organizer: 2015 ARVO Annual Meeting
  • Place of Presentation: Denver, Colorado
  • Year and Date: 2015-05-03 – 2015-05-07
• [Presentation] 病的網膜血管新生におけるＭｒｐ４の役割 (2015)
  • Author(s): 楠原仙太郎、松宮亘、根木昭
  • Organizer: 第118回 日本眼科学会総会
  • Place of Presentation: 札幌市
  • Year and Date: 2015-04-16 – 2015-04-19
• [Presentation] フォルスコリンはMrp4ノックアウトマウスにおける網膜血管形成を修飾する (2013)
  • Author(s): 松宮 亘、楠原仙太郎、根木 昭
  • Organizer: 第17回眼科分子生物学研究会
  • Place of Presentation: 箱根
• [Presentation] フォルスコリンはMrp4KO マウスにおける網膜血管形成を修飾する
  • Author(s): 松宮亘、楠原仙太郎、林部恵子、丸山和一、根木昭
  • Organizer: 第117回 日本眼科学会総会
  • Place of Presentation: 東京国際フォーラム