Development of an objective ophthalmic screening test by using the pupil light response

• Project/Area Number: 24791871
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Kitasato University
• Principal Investigator: ASAKAWA Ken 北里大学, 医療衛生学部, 助教 (60582749)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 瞳孔 / 対光反応 / 他覚的眼科検査法 / 視細胞 / 網膜神経節細胞 / 有色家兎 / 網膜電図 / 網膜組織所見 / 光‐電位変換 / melanopsin / pupil / retinal ganglion cell / rod / cone

Research Abstract

The aims of this research were both to evaluate the pupil light response mediated by the melanopsin-containing retinal ganglion cells and to examine the utility of the response as a new objective screening tool. We created specific wavelength blocking filters (95% block), also developed an innovative pupillography. We then evaluated the optimal conditions during the response activation of the melanopsin. Results showed markedly sustained pupil constriction in response to the 470 nm light for 10 seconds. The similar findings were also obtained both in an animal model with pharmacological damage of the cones and rods, and when using selective wavelength blocking filters. Of note, the pupil constriction was compatible with both the amplitudes of the electroretinography and the morphologic findings of the rabbit retina. The research suggests that the evaluation of the pupil response to colored-light stimulus can be used to diagnose diseases associated with the outer or inner retina.

Research Products

• [Journal Article] Abnormal pupillary light reflex with chromatic pupillometry in Gaucher disease (2014)
  • Author(s): Narita Aya , Shirai Kentarou , Kubota Norika , Takayama Rumiko , Takahashi Yukitoshi , Onuki Takanori , Numakura Chikahiko, Kato Mitsuhiro, Hamada Yusuke, Sakai Norio , Ohno Atsuko , Asami Maya , Matsushita Shoko , Hayashi Anri , Kumada Tomohiro, Fujii Tatsuya, Horino Asako, Inoue Takeshi, Kuki Ichiro, Asakawa Ken, Ishikawa Hitoshi, Ohno Koyo, Nishimura Yoko, Tamasaki Akiko , Maegaki Yoshihiro , Ohno Kousaku
  • Journal Title: Annals of Clinical and Translational Neurology Volume: 1(2) Pages: 135-140
  • Peer Reviewed
• [Journal Article] Abnormal pupillary light reflex with chromatic pupillometry in Gaucher disease (2014)
  • Author(s): Narita Aya，Shirai Kentarou，Kubota Norika，Takayama Rumiko，Takahashi Yukitoshi，Onuki Takanori，Numakura Chikahiko，Kato Mitsuhiro，Hamada Yusuke，Sakai Norio，Ohno Atsuko，Asami Maya，Matsushita Shoko，Hayashi Anri，Kumada Tomohiro，Fujii Tatsuya，Horino Asako， Inoue Takeshi，Kuki Ichiro，Asakawa Ken，et al
  • Journal Title: Annals of Clinical and Translational Neurology Volume: 1 Issue: 2 Pages: 135-140
  • DOI: 10.1002/acn3.33
  • Peer Reviewed
• [Journal Article] Evaluation of optic nerve head using a newly developed stereo retinal imaging technique by glaucoma specialist and non-expert certified orthoptist (2013)
  • Author(s): Asakawa Ken, Kato Sayaka, Shoji Nobuyuki, Morita Tetsuya, Shimizu Kimiya
  • Journal Title: Journal of Glaucoma Volume: 22(9) Pages: 698-706
  • Peer Reviewed
• [Journal Article] 神経眼科の進歩 -瞳孔とメラノプシンによる光受容- (2013)
  • Author(s): 石川均, 清水公也, 宇賀茂三, 吉冨健志, 浅川賢, 真下紀美代, 後関利明, 池田哲也, 庄司信行, 市邉義章, 近藤峰生, 寺崎浩子, 奥 英 弘 , 仲 泊 聡 , Patil Popat N , 丸 山 茂善, 中山茂信
  • Journal Title: 日本眼科学会雑誌 Volume: 117(3) Pages: 246-269
  • Peer Reviewed
• [Journal Article] 対光反射の起源・経路と中枢-近年の対光反射の考え- (2013)
  • Author(s): 石川均, 浅川賢
  • Journal Title: 神経内科 Volume: 78(2) Pages: 163-167
• [Journal Article] 神経眼科の進歩 -瞳孔とメラノプシンによる光受容- (2013)
  • Author(s): 石川均，清水公也，宇賀茂三，吉冨健志，浅川賢，真下紀美代，後関利明，池田哲也， 庄司信行，市邉義章，近藤峰生，寺崎浩子，奥英弘，仲泊聡，Patil Popat N，丸山茂善，中山茂信
  • Journal Title: 日本眼科学会雑誌 Volume: 117 Pages: 246-269
  • Peer Reviewed
• [Journal Article] Evaluation of optic nerve head using a newly developed stereo retinal imaging technique by glaucoma specialist and non-expert certified orthoptist (2013)
  • Author(s): Asakawa Ken，Kato Sayaka，Shoji Nobuyuki，Morita Tetsuya，Shimizu Kimiya
  • Journal Title: Journal of Glaucoma Volume: 22 Issue: 9 Pages: 698-706
  • DOI: 10.1097/ijg.0b013e318264be18
  • Peer Reviewed
• [Journal Article] 対光反射の起源・経路と中枢 －近年の対光反射の考え－ (2013)
  • Author(s): 石川均，浅川賢
  • Journal Title: 神経内科 Volume: 78 Pages: 163-167
• [Journal Article] 緑内障眼に おける立体眼底写真による視神経乳頭解析パラメータと Humphrey 視野計の視野指標との相関 (2012)
  • Author(s): 加藤紗矢香, 浅川賢, 庄司信行, 森田哲也, 永野幸一, 山口純, 清水公也
  • Journal Title: あたらしい眼科 Volume: 29(8) Pages: 1127-1130
  • NAID: 10031120805
  • Peer Reviewed
• [Journal Article] 瞳孔対光応答の波形特徴による眼疾患の推定に関する検討 (2012)
  • Author(s): 中山実, Nowak Wioletta, 石川均, 浅川賢, 市 邊 義 章
  • Journal Title: 信学技報 Volume: 111(482) Pages: 175-180
  • NAID: 110009546205
  • Peer Reviewed
• [Journal Article] 有色家兎の光受容器に対する 機能的評価と組織学的観察 (2012)
  • Author(s): 浅川賢, 石川均, 宇賀茂三, 真下紀美代, 清水公也
  • Journal Title: 日本眼科学会雑誌 Volume: 116(3) Pages: 348-349
• [Journal Article] Effects of selective-wavelength block filters on pupillary light reflex under red and blue light stimuli (2012)
  • Author(s): Ishikawa Hitoshi, Onodera Asami, Asakawa Ken, Nakadomari Satoshi, Shimizu Kimiya
  • Journal Title: Japanese Journal of Ophthalmology Volume: 56(2) Pages: 181-186
  • NAID: 10030248838
  • Peer Reviewed
• [Journal Article] 有色家兎の光受容器に対する機能的評価と組織学的観察 (2012)
  • Author(s): 浅川賢，石川均，宇賀茂三，真下紀美代，清水公也：
  • Journal Title: 日本眼科学会雑誌 Volume: 116 Pages: 348-349
• [Journal Article] Effects of selective-wavelength block filters on pupillary light reflex under red and blue light stimuli (2012)
  • Author(s): Ishikawa H, Onodera A, Asakawa K, Nakadomari S, Shimizu K
  • Journal Title: Jpn J Ophthalmol Volume: 56(2) Issue: 2 Pages: 181-186
  • DOI: 10.1007/s10384-011-0116-1
  • NAID: 10030248838
  • Peer Reviewed
• [Presentation] 変異型 ロ ドプシン遺伝子トランスジェニック家兎における網膜光受容器の機能的・組織学的評価 (2013)
  • Author(s): 浅川賢, 石川均, 宇賀茂三, Palisoc Erwin, 真下紀美代, 清水公也, 近藤峰生, 寺崎浩子
  • Organizer: 第51回日本神経眼科 学会
  • Place of Presentation: 秋田
• [Presentation] 正常者・不同視弱視患者における異なった条件下での光刺激による対光反射の検討 (2013)
  • Author(s): 戸塚悟, 石川均, 後関利明, 浅川賢, 池田哲也, 斎藤彩, 清水公也
  • Organizer: 第51回日本神経眼科学会
  • Place of Presentation: 秋田
• [Presentation] Functional and histological characterization of retinal photoreceptor cells in transgenic rabbit with rhodopsin-induced P347L mutation (2013)
  • Author(s): Asakawa Ken, Ishikawa Hitoshi, Palisoc Erwin, Mashimo Kimiyo, Uga Shigekazu, Shimizu Kimiya , Kondo Mineo , Terasaki Hiroko
  • Organizer: 7th Asia Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Bali
• [Presentation] The effect of zinc on ethambutol-induced toxic optic neuropathy-Functional and morphologic assessment in rabbit model- (2013)
  • Author(s): Palisoc Erwin, Ishikawa Hitoshi, Uga Shigekazu, Asakawa Ken, Mashimo Kimiyo, Shimizu Kimiya, Takano Masahiko, Shinkai Atsushi
  • Organizer: 7th Asia Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Bali
• [Presentation] 瞳孔とメラノプシン (2013)
  • Author(s): 浅川賢
  • Organizer: 第49回日本眼光学学会
  • Place of Presentation: 京都
• [Presentation] 神経型 Gaucher病における対光反射 (2012)
  • Author(s): 成田綾, 白井謙太朗, 前垣義弘, 久保田 智香, 高山留美子, 高橋幸利, 浅川賢, 石川均, 鈴木義之, 大野耕策
  • Organizer: 第11回アジア先天 代謝異常症シンポジウム
  • Place of Presentation: 岐阜
• [Presentation] 新生児と幼児における赤青光刺激による対光反射とサーカディアンリズム形成 (2012)
  • Author(s): 池田哲也, 石川均, 浅川賢, 戸塚悟, 後関利明, 高野雅彦, 清水公也
  • Organizer: 第50回日本神経眼科学会
  • Place of Presentation: 京都
• [Presentation] 健常者における瞳孔動揺の日内変動と自然光との関連性 (2012)
  • Author(s): 浅川賢, 石川均, 田ヶ谷浩邦, 清水公也
  • Organizer: 第50回日本神経眼科学会
  • Place of Presentation: 京都
• [Presentation] Nonmyd WX を用いた立体眼底画像による視神経乳頭解析の有用性 (2012)
  • Author(s): 浅川賢
  • Organizer: 第13回眼科臨床機器研究会
  • Place of Presentation: 横浜
• [Presentation] 薄暮視グレア下において遮光レンズがコントラスト感度へ与える影響 (2012)
  • Author(s): 金澤正継, 浅川賢, 魚里博
  • Organizer: 第48回日本眼光学学会
  • Place of Presentation: 東京
• [Presentation] Mechanism of photoreception in transgenic rabbit with rhodopsin P347L mutation (2012)
  • Author(s): Asakawa Ken , Ishikawa Hitoshi , Kondo Mineo
  • Organizer: 19th International Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Singapore
• [Presentation] 後房型有水晶体眼内レンズ (ICL TM )挿入術前後の対光反射 (2012)
  • Author(s): 藤原和子, 石川均, 神谷和孝, 浅川賢, 清水公也
  • Organizer: 第116回日本眼科学会総会
  • Place of Presentation: 東京
• [Presentation] 瞳孔とメラノプシン
  • Author(s): 浅川賢
  • Organizer: 第49回日本眼光学学会
  • Place of Presentation: ウェスティン都ホテル京都（京都府京都市）
  • Invited
• [Presentation] Functional and histological characterization of retinal photoreceptor cells in transgenic rabbit with rhodopsin-induced P347L mutation
  • Author(s): Asakawa Ken，Ishikawa Hitoshi，Palisoc Erwin，Mashimo Kimiyo，Uga Shigekazu
  • Organizer: 7th Asia Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Inna Grand Bali Beach Hotel（Bali， Indonesia）
• [Presentation] The effect of zinc on ethambutol-induced toxic optic neuropathy-Functional and morphologic assessment in rabbit model-
  • Author(s): Palisoc Erwin，Ishikawa Hitoshi，Uga Shigekazu，Asakawa Ken，Mashimo Kimiyo，Shimizu Kimiya，Takano Masahiko，Shinkai Atsushi
  • Organizer: 7th Asia Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Inna Grand Bali Beach Hotel（Bali， Indonesia）
• [Presentation] 正常者・不同視弱視患者における異なった条件下での光刺激による対光反射の検討
  • Author(s): 戸塚悟，石川均，後関利明，浅川賢，池田哲也，斎藤彩，清水公也
  • Organizer: 第51回日本神経眼科学会
  • Place of Presentation: 秋田拠点センターALVE（秋田県秋田市）
• [Presentation] 変異型ロドプシン遺伝子トランスジェニック家兎における網膜光受容器の機能的・組織学的評価
  • Author(s): 浅川賢，石川均，宇賀茂三，Palisoc Erwin，真下紀美代，清水公也，近藤峰生，寺崎浩子
  • Organizer: 第51回日本神経眼科学会
  • Place of Presentation: 秋田拠点センターALVE（秋田県秋田市）
• [Presentation] 新生児と幼児における赤青光刺激による対光反射とサーカディアンリズム形成
  • Author(s): 池田哲也，石川均，浅川賢，戸塚悟，後関利明，高野雅彦，清水公也
  • Organizer: 第50回日本神経眼科学会
  • Place of Presentation: 京都
• [Presentation] 健常者における瞳孔動揺の日内変動と自然光との関連性
  • Author(s): 浅川賢，石川均，田ヶ谷浩邦，清水公也
  • Organizer: 第50回日本神経眼科学会
  • Place of Presentation: 京都
• [Presentation] 薄暮視グレア下において遮光レンズがコントラスト感度へ与える影響
  • Author(s): 金澤正継，浅川賢，魚里博
  • Organizer: 第48回日本眼光学学会
  • Place of Presentation: 東京
• [Presentation] Mechanism of photoreception in transgenic rabbit with rhodopsin P347L mutation
  • Author(s): Ken Asakawa，Hitoshi Ishikawa，Mineo Kondo
  • Organizer: 19th International Neuro-Ophthalmology Society Meeting
  • Place of Presentation: Singapore
• [Book] 瞳孔検査.眼科ケア 14 (10) (2012)
  • Author(s): 浅川賢, 石川均
  • Publisher: メディカ出版