To analyze the transport between the outer retina and the choroidal circulation in Age-related macular degeneration

• Project/Area Number: 24791876
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ophthalmology
• Research Institution: Keio University
• Principal Investigator: IZUMI-NAGAI Kanako 慶應義塾大学, 医学部, 共同研究員 (60407103)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 網膜脈絡膜物質輸送 / 加齢黄斑変性 / fenestrae / 脈絡膜毛細血管

Research Abstract

To analyze in vivo tracer transport through the murine choriocapillaris, cardiac or tail vein injection using dextran , NHS-Biotin or transferrin to wild mice was done. The leakage outside of the choriocapillaris was observed immediately after injection with NHS-Biotin. On the other hand, punctate staining pattern was observed using dextran (70KDa) or transferrin. These results suggested that transport of small molecule was fenestrae-dependent and that of large molecule was endocytosis-dependent.