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In vivo real-time visualization of therapeutic pharmacodynamics in Crohn's disease model mice using two photon laser scanning microscopy

Research Project

Project/Area Number 24791887
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Pediatric surgery
Research InstitutionMie University

Principal Investigator

INOUE Mikihiro  三重大学, 医学部附属病院, 助教 (30422835)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords二光子レーザー顕微鏡 / クローン病 / 生体内イメージング / 二光子励起顕微鏡
Research Abstract

We attempted to visualize and analyze in vivo therapeutic response of infliximab (IFX) in the murine IBD model using two photon laser scanning microscopy (TPLSM).
The number of leukocytes infiltrating to subserosa and muscular layer was reduced in IFX-treated mice, compared with non-IFX, control mice. The length of colonic crypt was increased in IFX-treated mice, compared with non-IFX, control mice. Serial change in regenerative epithelial cells was not observed, which regenerated from the bottom of the crypt toward the top of the crypt (the luminal side). The fluorescence labeled anti-TNF-alpha antibody was observed in the mucosal stroma, suggesting the binding of labeled antibodies with free TNF- alpha in the colonic tissue. It was also observed in the surface of leukocytes within the postcapillary venules or those infiltrating into the damaged crypt, suggesting the binding of labeled antibodies with the membrane binding TNF- alpha of the surface TNF- alpha producing cells.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (32 results)

All 2014 2013 2012

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (14 results)

  • [Journal Article] The impact of postoperative infliximab maintenance therapy on preventing the surgical recurrence of Crohn's disease : a single-center paired case-control study2014

    • Author(s)
      Araki T, Uchida K, Okita Y, Fujikawa H, Inoue M, Ohi M, Tanaka K, Inoue Y, Mohri Y, Kusunoki M
    • Journal Title

      Surg Today

      Volume: 44(2) Pages: 291-296

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Infantile Crohn's disease is one of the risk factors of Catheter-related Blood Stream Infection2014

    • Author(s)
      Koike Y, Uchida K, Inoue M, Ide S, Hashimoto K, Matsushita K, Otake K, Ohkita Y, Araki T, Kobayashi M, Mohri Y, Kusunoki M
    • Journal Title

      Pediatr Int

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sexual activity after ileal pouch-anal anastomosis in Japanese patients with ulcerative colitis2014

    • Author(s)
      Yoshida K, Araki T, Uchida K, Okita Y, Fujikawa H, Inoue M, Tanaka K, Inoue Y, Mohri Y, Kusunoki M
    • Journal Title

      Surg Today

      Volume: 44(1) Pages: 73-79

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Infantile Crohn's disease is one of the risk factors of Catheter-related Blood Stream Infection.2014

    • Author(s)
      Koike Y, Uchida K, Inoue M, Ide S, Hashimoto K, Matsushita K, Otake K, Ohkita Y, Araki T, Kobayashi M, Mohri Y, Kusunoki M.
    • Journal Title

      Pediatr Int.

      Volume: Feb 12 Issue: 3 Pages: 364-368

    • DOI

      10.1111/ped.12304

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Predictive factors of negative outcome in initial atropine therapy for infantile hypertrophic pyloric stenosis2013

    • Author(s)
      Koike Y, Uchida K, Nakazawa M, Inoue M, Kusunoki M, Tsukamoto Y
    • Journal Title

      Pediatr Int

      Volume: 55(5) Pages: 619-623

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Predictive Factors for Development of Chronic Pouchitis after Ileal Pouch-Anal Anastomosis in Ulcerative Colitis2013

    • Author(s)
      Okita Y, Araki T, Tanaka K, Hashimoto K, Kondo S, Kawamura M, Koike Y, Otake K, Fujikawa H, Inoue M, Ohi M, Inoue Y, Uchida K, Mohri Y, Kusunoki M
    • Journal Title

      Digestion

      Volume: 88(2) Pages: 101-109

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of Seprafilm for preventing adhesive bowel obstruction and cost-benefit analysis in pediatric patients undergoing laparotomy2013

    • Author(s)
      Inoue M, Uchida K, Otake K, Nagano Y, Ide S, Hashimoto K, Matsushita K, Koike Y, Mohri Y, Kusunoki M
    • Journal Title

      J Pediatr Surg

      Volume: 48(7) Pages: 1528-1534

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Primary liver transplantation for biliary atresia after percutaneous transhepatic cholangio-drainage2013

    • Author(s)
      Koike Y, Uchida K, Hashimoto K, Matsushita K, Otake K, Inoue M, Kusunoki M
    • Journal Title

      Journal of Pediatric Surgery Case Reports

      Volume: Vol.1,Issue7 Pages: 183-185

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hairy polyp can be lethal even when small in size2013

    • Author(s)
      Koike Y, Uchida K, Inoue M, Ohtsu K, Tanaka T, Otake K, Tanaka K, Kusunoki M
    • Journal Title

      Pediatr Int

      Volume: 55(3) Pages: 373-376

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Scar endometriosis in a patient with bladder exstrophy2013

    • Author(s)
      Kitajima T, Inoue M, Uchida K, Otake K, Kusunoki M
    • Journal Title

      Int Surg

      Volume: 98(2) Pages: 145-148

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characteristics of extremely early-onset pouchitis after proctocolectomy with ileal pouch-anal anastomosis2013

    • Author(s)
      Okita Y, Araki T, Tanaka K, Shimura T, Kawamura M, Fujikawa H, Inoue M, Inoue Y, Uchida K, Mohri Y, Kusunoki M
    • Journal Title

      J Gastrointest Surg

      Volume: 17(3) Pages: 533-539

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Self-reported assessment of health-related quality of life in children who underwent restorative proctocolectomy with ileal J-pouch anal anastomosis for ulcerative colitis2013

    • Author(s)
      Uchida K, Kawamata A, Hashimoto K, Inoue M, Otake K, Koike Y, Matsushita K, Fujikawa H, Okita Y, Araki T, Tanaka K, Kusunoki M
    • Journal Title

      Pediatr Surg Int

      Volume: 29(3) Pages: 287-291

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transumbilical single-incision laparoscopic ileocecal resection for Crohn's disease in a child2013

    • Author(s)
      Inoue M, Uchida K, Otake K, Okigami M, Okita Y, Hiro J, Araki T, Kusunoki M
    • Journal Title

      Asian J Endosc Surg

      Volume: 6(1) Pages: 48-51

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficacy of Seprafilm for preventing adhesive bowel obstruction and cost-benefit analysis in pediatric patients undergoing laparotomy.2013

    • Author(s)
      Inoue M, Uchida K, Otake K, Nagano Y, Ide S, Hashimoto K, Matsushita K, Koike Y, Mohri Y, Kusunoki M.
    • Journal Title

      J Pediatr Surg.

      Volume: 48 Issue: 7 Pages: 1528-1534

    • DOI

      10.1016/j.jpedsurg.2013.01.028

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transumbilical single-incision laparoscopic ileocecal resection for Crohn's disease in a child.2013

    • Author(s)
      Inoue M et al
    • Journal Title

      Asian J Endosc Surg.

      Volume: 6 Issue: 1 Pages: 48-51

    • DOI

      10.1111/j.1758-5910.2012.00162.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Double balloon enteroscopy for pediatric inflammatory bowel disease2012

    • Author(s)
      Uchida K, Yoshiyama S, Inoue M, Koike Y, Yasuda H, Fujikawa H, Okita Y, Araki T, Tanaka K, Kusunoki M
    • Journal Title

      Pediatr Surg Int

      Volume: 54(6) Pages: 806-809

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unique capsule endoscopic appearance of segmental intestinal dilatation in a child2012

    • Author(s)
      Uchida K, Otake K, Inoue M, Koike Y, Matsushita K, Hashimoto K, Saigusa S, Tanaka K, Inoue Y, Kusunoki M
    • Journal Title

      Pediatr Int

      Volume: 54(5) Pages: 727-729

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Use of Endoscopic Surgical Spacer to Improve Safety During Thoracoscopic Repair of Congenital Diaphragmatic Hernia2012

    • Author(s)
      Inoue M, Uchida K, Otake K, Ishino Y, Koike Y, Kusunoki M
    • Journal Title

      J Laparoendosc Adv Surg Tech A

      Volume: 22(3) Pages: 304-306

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 小児における小腸ダブルバルーン内視鏡施行時の放射線被爆2013

    • Author(s)
      井上 幹大, 内田 恵一, 井出 正造, 今岡 裕基, 小池 勇樹, 大竹耕平, 楠 正人
    • Organizer
      第40回日本小児内視鏡研究会
    • Place of Presentation
      聖マリアンナ医科大学
    • Year and Date
      2013-07-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 小児炎症性腸疾患に対する単孔式内視鏡手術と Reduced Port Surgery2013

    • Author(s)
      井上 幹大, 内田 恵一, 廣 純一郎, 森 浩一郎, 井出正造, 小池 勇樹, 藤川 裕之, 大竹 耕平, 大北 喜基, 荒木 俊光, 楠 正人
    • Organizer
      第26回日本内視鏡外科学会総会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] 大腸手術における Reduced Port Surgeryの意義と定義 大腸全摘出・J 型回腸嚢肛門吻合術に対する Reduced Port Surgeryの意義2013

    • Author(s)
      廣 純一郎, 荒木 俊光, 井上 靖浩, 大北 喜基, 藤川 裕之, 川本 文, 井上 幹大, 問山 裕二, 田中 光司, 毛利靖彦, 楠 正人
    • Organizer
      第26回日本内視鏡外科学会総会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] 二光子励起顕微鏡を用いたマウス敗血症モデルにおけるNeutrophil Extracellular Traps の生体内可視化2013

    • Author(s)
      田中 光司, 小池 勇樹, 志村 匡信, 井出正造, 今岡 裕基, 近藤 哲, 北嶋 貴仁, 問山 裕二, 小林 美奈子, 井上 幹大, 井上 靖浩, 荒木 俊光, 内田 恵一, 毛利靖彦, 楠 正人
    • Organizer
      第26回日本外科感染症学会総会
    • Place of Presentation
      神戸国際会議場二光子励起顕微鏡を用いたマウス敗血症モ デ ル に お け る Neutrophil Extracellular Traps の生体内可視化
    • Related Report
      2013 Final Research Report
  • [Presentation] 二光子励起顕微鏡を用いたマウス敗血症モデルにおける組織末梢循環不全とAntithrombin III 製剤効果の生体内可視化2013

    • Author(s)
      田中 光司, 志村 匡信, 井出 正造, 今岡 裕基, 近藤 哲, 北嶋 貴仁, 問山裕二, 奥川 喜永, 小林 美奈子, 井上 幹大, 井上 靖浩, 荒木 俊光, 内田 恵一, 毛利 靖彦, 楠 正人
    • Organizer
      第75回日本臨床外科学会総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Final Research Report
  • [Presentation] クローン病の肛門病変の治療と成績 新生児・乳児期発症クローン病の肛門病変と治療2013

    • Author(s)
      内田 恵一, 井上 幹大, 大竹 耕平, 小池 勇樹, 藤川 裕之, 大北 喜基, 荒木 俊光, 廣 純一郎, 大井 正貴, 問山 裕二, 三枝 晋, 田中 光司, 井上 靖浩, 毛利 靖彦, 楠 正人
    • Organizer
      第68回日本大腸肛門病学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Related Report
      2013 Final Research Report
  • [Presentation] 回盲部切除後の寛解維持療法としてインフリキシマブ投与を行った小児クローン病症例の検討2013

    • Author(s)
      井上 幹大, 内田 恵一, 井出 正造, 橋本 清, 小池 勇樹, 藤川 裕之, 大北 喜基, 大竹 耕平, 荒木 俊光, 楠 正人
    • Organizer
      第50回日本小児外科学会学術集会
    • Place of Presentation
      京王プラザホテル回
    • Related Report
      2013 Final Research Report
  • [Presentation] 小児内視鏡手術の展望小児に対するReduced Port Surgery による腹腔鏡下大腸全摘回腸嚢肛門吻合術2013

    • Author(s)
      井上 幹大, 廣 純一郎, 井出 正造, 橋本 清, 小池 勇樹, 藤川 裕之, 大竹 耕平, 大北 喜基, 荒木俊光, 内田 恵一, 楠 正人
    • Organizer
      第50回日本小児外科学会学術集会
    • Place of Presentation
      京王プラザホテル
    • Related Report
      2013 Final Research Report
  • [Presentation] 小児炎症性腸疾患に対する単孔式内視鏡手術とReduced Port Surgery2013

    • Author(s)
      井上 幹大, 内田 恵一, 廣 純一郎, 森 浩一郎, 井出 正造, 小池 勇樹, 藤川 裕之, 大竹 耕平, 大北 喜基, 荒木 俊光, 楠 正人
    • Organizer
      第26回日本内視鏡外科学会総会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 単孔式内視鏡手術にて回盲部切除を施行した小児クローン病の1例2013

    • Author(s)
      井上 幹大, 内田 恵一, 大竹 耕平, 松下 航平, 大北 喜基, 吉山 繁幸, 廣 純一郎, 荒木 俊光, 楠 正人
    • Organizer
      第40回日本小児栄養消化器肝臓学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 二光子励起顕微鏡を用いたマウス敗血症モデルにおけるNeutrophil Extracellular Trapsの生体内可視化2013

    • Author(s)
      田中 光司, 小池 勇樹, 志村 匡信, 井出 正造, 今岡 裕基, 近藤 哲, 北嶋 貴仁, 問山 裕二, 小林 美奈子, 井上 幹大, 井上 靖浩, 荒木 俊光, 内田 恵一, 毛利 靖彦, 楠 正人
    • Organizer
      日本外科感染症学会
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 二光子励起顕微鏡を用いたマウス敗血症モデルにおける組織末梢循環不全とAntithrombin III製剤効果の生体内可視化2013

    • Author(s)
      田中光司、志村匡信、井出正造、今岡裕基、近藤 哲、北嶋貴仁、問山裕二、奥川喜永、小林美奈子、井上幹大、井上靖浩、荒木俊光、内田恵一、毛利靖彦、楠 正人
    • Organizer
      第75回日本臨床外科学会総会
    • Place of Presentation
      名古屋国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 小児外科でのSILS 小児クローン病に対する単孔式内視鏡手術2012

    • Author(s)
      井上幹大
    • Organizer
      第74回日本臨床外科学会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 小児期クローン病に対するダブルバルーン内視鏡施行の意義2012

    • Author(s)
      井上幹大
    • Organizer
      第54回日本消化器病学会大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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