| Project/Area Number |
24791893
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNAメチル化 / 神経芽腫 / 腫瘍関連遺伝子 / 小児外科 / 小児固形腫瘍 / メチル化 |
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| Research Abstract |
To find new neuroblastoma related genes, we investigated DNA methylation level and expression level of the tumor-related genes, which have been screened from global analysis of genome DNA and transcripts obtained from mouse skin cancers, in the neuroblastoma specimens. And then we examined the association between the aberrance of those genes and clinical data. In this study, we found that TFAP2E showed lower methylation level and higher expression level in the neuroblastoma samples compared to normal adrenal tissues. In addition, our data indicated that its expression was controlled by DNA methylation of CpG island-1. In the survival analysis, it was clearly shown that the lower methylation level of CpG island-1 was associated with the worse prognosis. Our present data strongly suggested that TFAP2E could be a new neuroblastoma related gene.
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