Evaluation of culture cartilage stem / progenitor cells based on the innovative three-dimensional culture techniques that mimics the in vivo environment

Research Project

Project/Area Number	24791911
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Plastic surgery
Research Institution	Yokohama City University
Principal Investigator	YABUKI Yuichiro 横浜市立大学, 医学(系)研究科(研究院), 客員研究員 (30610357)
Research Collaborator	MAEGAWA Jiro KOBAYASHI Shinji TAKEBE Takanori KAMIJO Aki TANIGUCHI Hideki
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	軟骨幹／前駆細胞 / 三次元培養 / 自家血清 / 再生医療 / RWV / 軟骨幹細胞 / 軟骨前駆細胞
Outline of Final Research Achievements	I produced perichondrocytes and serum from the provided perichondrium and blood. The perichondrium cells are culture expanded with autologous serum (AS) containing media and FBS-containing media and subjected to the analysis. The former is shorter cell doubling time in the AS use group and showed good growth. However, any of the group also was able to equally cartilage differentiation. In vivo experiments in severe immunodeficient mice showed the regeneration of the equivalent elastic cartilage-like tissue. The expanded perichondrocytes were inserted three-dimensional culture, RWV. Regenerated tissue is compared, and it was stable in shape in FBS group. But admitted the regenerated cartilage-like tissue in any of the group in the histologically, it was those immature.