Elucidation of peripheral nerve regeneration-promoting mechanism and in vivo kinetics of transplanted adipose-derived stem cells for medical applications
| Project/Area Number |
24791913
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
SOWA yoshihiro 京都府立医科大学, 医学(系)研究科(研究院), 助教 (80468264)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脂肪組織由来幹細胞 / 再生医療 / 体性幹細胞 / 神経堤 / 脂肪前駆細胞 / 細胞分化 / 脂肪由来幹細胞 / 発生学 / 神経堤細胞 / 間葉系幹細胞 / 末梢神経 / 幹細胞 / 脂肪 / 末梢神経再生 / 脂肪分化 |
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| Outline of Final Research Achievements |
In the present study, we aimed at investigating how and to what extent the neural crest contributes to ASCs using Cre-loxP-mediated fate mapping. ASCs harvested from subcutaneous fat depots of either adult P0-Cre/ or Wnt1-Cre/ Floxed-reporter mice contained a few neural crest-derived ASCs (NCDASCs). This subpopulation of cells exhibited a unique bipolar or multipolar morphology with higher expression of neural crest and preadipocyte markers and differentiate into adipocytes with high efficiency, indicating their commitment to adipogenesis. In vivo, a very small proportion of adipocytes were originated from the neural crest. These results demonstrate that ASCs contains neural crest-derived adipogenic progenitors whose phenotype is distinct from that of non-neural crest derivatives.
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Report
(4 results)
Research Products
(17 results)
