| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In the present study, we aimed at investigating how and to what extent the neural crest contributes to ASCs using Cre-loxP-mediated fate mapping. ASCs harvested from subcutaneous fat depots of either adult P0-Cre/ or Wnt1-Cre/ Floxed-reporter mice contained a few neural crest-derived ASCs (NCDASCs). This subpopulation of cells exhibited a unique bipolar or multipolar morphology with higher expression of neural crest and preadipocyte markers and differentiate into adipocytes with high efficiency, indicating their commitment to adipogenesis. In vivo, a very small proportion of adipocytes were originated from the neural crest. These results demonstrate that ASCs contains neural crest-derived adipogenic progenitors whose phenotype is distinct from that of non-neural crest derivatives.
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