| Project/Area Number |
24791950
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
HATANO Takaharu 大阪市立大学, 医学(系)研究科(研究院), 研究員 (10382144)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 熱傷深達度 / 熱傷モデル / レーザースペクル画像血流計 / 熱傷 / レーザースペックル画像血流計 / 深度 |
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| Research Abstract |
[Introduction] It is often difficult to determine the depth of burns correctly. In this study, we prepared models of superficial dermal burns (SDB) and deep dermal burns (DDB) in rats, determined the burn depth by using laser speckle flowgraphy (LSFG), and examined whether or not we could distinguish between SDB and DDB.[Materials and Methods] Male Wistar rats aged 10 weeks were used and burns were created by the hot water method. Models of SDB and DDB were defined based on examination of specimens stained with hematoxylin-eosin. In these burn models, blood flow was measured over time using LSFG. [Results] Since LSFG measurements are relative values, the ratio of blood flow in the burned skin to that in the normal skin was calculated. This blood flow ratio determined by LSFG showed a significant difference between SDB and DDB. [Discussion] LSFG could be a useful method for the evaluation of wound blood flow in patients with burns.
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