| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Outline of Final Research Achievements |
The production of reactive oxygen species (ROS) and the resulting oxidative stress in mice in response to a controlled cortical impact (CCI) are typical exacerbating factors associated with traumatic brain injury (TBI).The aim of the present study was to determine whether PACAP38 exerts neuroprotective effects by regulating oxidative stress in mice with TBI. PACAP38 was administered intravenously immediately following CCI, and immunostaining for the oxidative stress indicator nitrotyrosine, and for neuronal death were measured 24 hours later. Western blot experiments to determine antioxidant activity in the neocortical region were also performed three hours post-CCI. . PACAP38 suppressed the extent of TBI and NT-positive regions 24 hours after CCI, and increased antioxidant enzymes in both hemispheres. Taken together, these results suggest that increasing antioxidant might activity isbe involving in one of the neuroprotective effect of PACAP38 in mice subjected to a CCI.
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