PACAP38 suppresses cortical damage in mice with traumatic brain injury by enhancing antioxidant activity.
| Project/Area Number |
24791954
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Emergency medicine
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| Research Institution | Showa University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
OHTAKI hirokazu 昭和大学, 医学部 顕微解剖学, 講師 (20349062)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | 頭部外傷 / PACAP38 / 活性酸素種 / 酸化ストレス / 二次性損傷 / 神経保護作用 / 抗酸化酵素 / 抗酸化 / 抗酸化力 / 抗酸化能 / 神経損傷 / 神経保護 / 抗酸化作用 / Western blotting / PAC-1 recepter |
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| Outline of Final Research Achievements |
The production of reactive oxygen species (ROS) and the resulting oxidative stress in mice in response to a controlled cortical impact (CCI) are typical exacerbating factors associated with traumatic brain injury (TBI).The aim of the present study was to determine whether PACAP38 exerts neuroprotective effects by regulating oxidative stress in mice with TBI. PACAP38 was administered intravenously immediately following CCI, and immunostaining for the oxidative stress indicator nitrotyrosine, and for neuronal death were measured 24 hours later. Western blot experiments to determine antioxidant activity in the neocortical region were also performed three hours post-CCI. . PACAP38 suppressed the extent of TBI and NT-positive regions 24 hours after CCI, and increased antioxidant enzymes in both hemispheres. Taken together, these results suggest that increasing antioxidant might activity isbe involving in one of the neuroprotective effect of PACAP38 in mice subjected to a CCI.
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Report
(5 results)
Research Products
(56 results)
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[Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) contributes to the proliferation of hematopoietic progenitor cells in murine bone marrow via PACAP-specific receptor.2016
Author(s)
Xu Z, Ohtaki H, Watanabe J, Miyamoto K, Murai N, Sasaki S, Matsumoto M, Hashimoto H, Hiraizumi Y, Numazawa S, Shioda S.
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Journal Title
Sci Rep
Volume: 6
Issue: 1
Pages: 22373-22373
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] PACAP38 suppresses cortical damage in mice with traumatic brain injury by enhancing antioxidant activity.2014
Author(s)
Miyamoto K, Tsumuraya T, Ohtaki H, Dohi K, Satoh K, Xu Z, Tanaka S, Murai N, Watanabe J, Sugiyama K, Aruga T, Shioda S.
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Journal Title
J Mol Neurosci.
Volume: 54(3)
Issue: 3
Pages: 370-379
DOI
Related Report
Peer Reviewed
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[Journal Article] PACAP38 suppresses cortical damage in mice with traumatic brain injury by enhancing antioxidant activity.2014
Author(s)
Miyamoto K, Tsumuraya T, Ohtaki H, Dohi K, Satoh K, Xu Z, Tanaka S, Murai N, Watanabe J, Sugiyama K, Aruga T and Shioda S
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Journal Title
Journal of molecular neuroscience
Volume: In press
Related Report
Peer Reviewed
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[Journal Article] Establishment and Characterization of Primary Adult Microglial Culture in Mice2013
Author(s)
Ohtaki H, Tsumuraya T, Song D, Sato A, Ohara K, Miyamoto K, Nakano H, Kiriyama K, Dohi K, Hiraizumi H, Matsunaga M, Shioda S
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Journal Title
Acta Neurochir Suppl
Volume: 118
Pages: 49-54
DOI
ISBN
9783709114339, 9783709114346
Related Report
Peer Reviewed
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[Presentation] 多臓器不全を合併したスズメバチ多数刺傷の1例2016
Author(s)
宮本和幸, 笹井文彦, 林将史, 橋詰真衣, 高安弘美, 原田芳邦, 前田敦雄, 河面倫有, 渡邉兼正, 佐々木純, 林宗貴.
Organizer
第66回日本救急医学会関東地方会
Place of Presentation
都市センターホテル(東京)
Year and Date
2016-02-06
Related Report
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[Presentation] 肝機能障害・横紋筋融解症を合併したスズメバチ刺傷の1例2016
Author(s)
宮本和幸, 笹井文彦, 林将史, 橋詰真衣, 高安弘美, 原田芳邦, 前田敦雄, 河面倫有, 渡邉兼正, 佐々木純, 林宗貴.
Organizer
第30回日本中毒学会東日本地方会
Place of Presentation
昭和大学上條講堂(東京)
Year and Date
2016-01-23
Related Report
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[Presentation] マウス熱射病モデルにおける経口補水液の検討2014
Author(s)
宮本和幸, 大滝博和, 海老原直樹, 山本大輔, 川口絢子, 井手亮太, 山下智幸, 橋本香織,田中啓司, 中村俊介 ,三宅康史, 有賀徹
Organizer
第42回日本救急医学会総会
Place of Presentation
福岡国際会議場・福岡サンパレスホテル・ホール(福岡)
Year and Date
2014-10-28 – 2014-10-30
Related Report
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[Book] 救急医学2015
Author(s)
山本大輔, 宮本和幸
Total Pages
128
Publisher
へるす出版
Related Report
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