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A relation histamine receptor and vascular permeability in the model of lung microvascular with endotoxemia.

Research Project

Project/Area Number 24791956
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Emergency medicine
Research InstitutionHyogo Medical University

Principal Investigator

OSAKO TAKAAKI  兵庫医科大学, 医学部, 助教 (30573844)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsヒスタミン / 血管透過性 / 多価不飽和脂肪酸 / ARDS / HMVEC / 国際情報交換
Outline of Final Research Achievements

During acute lung inflammation, the lung microvasculature becomes hyperpermeable, resulting in immune cell infiltration and tissue edema. In this study, we examined the effects n-3 PUFAs onlung microvascular cell permeability.Human lung microvascular endothelial cells (HMVEC-L) were seeded on fibronectin-coated transwell inserts. The cells were pretreated with docosahexaenoic acid (DHA) or eicosapentaenoic acid (EPA) (n-3 PUFAS), and then treated with LPS to simulate acute lung injury. Pretreatment with DHA and EPA prior to LPS stimulation significantly attenuated LPS-induced cell permeability. EPA decreased histamine receptor 1 (H1R) and mRNA expression following LPS stimulation. Interleukin (IL)-6 mRNA expression in response to LPS treatment was significantly reduced by both DHA and EPA pretreatment. DHA and EPA attenuated LPS-induced lung microvascular endothelial cell permeability through a mechanism that may involve IL-6. EPA pretreatment may influence H1R expression.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (1 results)

All 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Eicosapentaenoic Acid Decreases Histamine Receptor 1 Expression on Lung Microvascular Endothelial Cells and Cell Permeability during LPS stimulation.2014

    • Author(s)
      Takaaki Osako, Michiko Aoyama-Ishikawa, Hayato Yamashita, Makoto Usami, Atsunori Nakao, Joji Kotani
    • Journal Title

      International Journal of Respiratory and Pulmonary Medicine

      Volume: 1 Pages: 1-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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