Study of tooth morphogenesis from the aspect of local regulation of phosphate metabolism: An epigenomic approach.

• Project/Area Number: 24791962
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: YOSHIOKA Hirotaka 広島大学, 医歯薬保健学研究院(歯), 助教 (50523411)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: エピジェネティクス / 歯胚 / リン代謝

Research Abstract

We have performed histological and molecular experiment to uncover the distribution of epigenetic modifications, especially DNA methylation and hydroxymethylation, and related factors during mouse amelogenesis. The global increase of 5-hydroxymethylcytosine levels was observed when the ameloblast cells enter into secretory stage; then onset of differentiation into secretory stage, the formation of chromocenter was observed. Also, Dnmt1 mRNA and protein expression showed highest levels in immature dental epithelial cells, with decreasing levels at later stages of development. These results suggest that Dnmt1 may sustain the maintenance of undifferentiation status of dental epithelial cells.

Research Products

• [Journal Article] Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene (2013)
  • Author(s): Yang Z, Yoshioka H, McCarrey JR
  • Journal Title: Reproduction Volume: 146 Issue: 5 Pages: 501-516
  • DOI: 10.1530/rep-13-0311
  • Peer Reviewed
• [Presentation] マウス歯胚発生過程におけるエピジェネティクス制御機構の解明 (2013)
  • Author(s): 吉岡広陽, 南崎朋子, 吉子裕二
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-21
• [Presentation] マウス歯胚発生過程におけるDNA メチル化酵素Dnmt1 の役割 (2013)
  • Author(s): 大西梓, 吉岡広陽, 南崎朋子, 吉子裕二
  • Organizer: 第118回日本解剖学会総会・学術集会
  • Place of Presentation: 高松
  • Year and Date: 2013-03-28
• [Presentation] Soluble Klotho does not rescue but rather exaggerates skeletal defects in Klotho-deficient mice (2013)
  • Author(s): Minamizaki T, Konishi Y, Sakurai K, Yoshioka H, Kozai K, Yoshiko Y
  • Organizer: 5th Hiroshima Conference on Education and Science in Dentistry
  • Place of Presentation: Hiroshima
• [Presentation] Imaging mass spectrometry-based molecular histology of bone shows the implication of MEPE-ASARM for the Klotho-deficient phenotype (2013)
  • Author(s): Fujino Y, Minamizaki T, Sakurai K, Irie Y, Yoshioka H, Takei Y, Kozai K, Okada M, Yoshiko Y
  • Organizer: The American Society for Bone and Mineral Research 2013 Annual Meeting
  • Place of Presentation: Baltimore, USA
• [Presentation] Soluble Klotho does not rescue but rather exaggerates skeletal defects in Klotho-deficient mice (2013)
  • Author(s): Minamizaki T, Konishi Y, Sakurai K, Yoshioka H, Kozai K, Yoshiko Y
  • Organizer: 2nd Joint Meeting of International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe
• [Presentation] Soluble Klotho does not rescue but rather exaggerates skeletal defects in klotho-deficient mice. (2013)
  • Author(s): Minamizaki T, Konishi Y, Sakurai K, Yoshioka H, Kozai K, Yoshiko Y.
  • Organizer: 2nd Joint Meeting of International Bone and Mineral Society and The Japanese Society for Bone and Mineral Research
  • Place of Presentation: Kobe, Japan
• [Presentation] Imaging Mass Spectrometry-based Molecular Histology of Bone Shows the Implication of MEPE-ASARM for the Klotho-Deficient Phenotype. (2013)
  • Author(s): Fujino Y, Minamizaki T, Sakurai K, Irie Y, Yoshioka H, Takei Y, Kozai K, Okada M, Yoshiko Y.
  • Organizer: The American Society for Bone and Mineral Research 2013 Annual Meeting
  • Place of Presentation: Baltimore, MD, USA
• [Presentation] マウス歯胚発生過程におけるDNAメチル化酵素Dnmt1の役割 (2013)
  • Author(s): 大西 梓，吉岡 広陽，南崎 朋子，吉子 裕二
  • Organizer: 第118回 日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川県高松市
• [Presentation] Transient but not constitutive activation of ERK is necessary for the unique action of FGF23 in bone (2012)
  • Author(s): Minamizaki T, Konishi Y, Yoshioka H, Kozai K, Yoshiko Y
  • Organizer: The 34th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: Minneapolis, USA
• [Presentation] マウス歯胚発生過程におけるエピジェネティクス制御機構の解明
  • Author(s): 吉岡広陽，南崎朋子，吉子裕二
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山県岡山市
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/anato1/welcome.htm