| Project/Area Number |
24791967
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
OTSU Keishi 岩手医科大学, 歯学部, 助教 (60509066)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | エナメル芽細胞 / 分化 / Rho / ROCK / EMT / エナメル質 / 歯 / 幹細胞 / Rhoシグナリング / Semaphorin / slug / sox2 |
|---|
| Research Abstract |
In this study, we examined the role of Rho signaling in ameloblasts differentiation and stem cell maintenance. The experiments using mice with epithelial specific expression of RhoA dominant negative demonstrated that Rho signaling regulated cytoskeletal dynamics and cell adhesion during ameloblasts differentiation. Semaphorin 4D-PlexinB1-LARG signal cascade controlled Rho A activity. Further, since RhoA activity regulated the expression of slug, the transcription factor that controlled epithelial-mesenchymal transition (EMT), it was suggested that EMT regulated ameloblasts differentiation and stem cell maintenance via Rho signaling.
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