Interaction of erbB and AID in development/local invasion of odontogenic tumor
| Project/Area Number |
24791968
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 歯原性腫瘍 / エナメル上皮腫 / erbB / AID / 局所侵襲 / ameloblastoma / 研究及び総括 |
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| Outline of Final Research Achievements |
In order to reveal the mechanism of occurrence and local invasion on ameloblastoma, the interaction of gene editing enzyme AID and transmembrane protein erbB were studied. Immunohistochemistry showed that erbB2, AID and 5-mc express strongly in plexiform type of ameloblastoma. To confirm the DNA methylation, bisulfate-PCR reaction against the isolated DNA from segments were performed. However, this experiment did not show the clear result. Then, odontogenic like epithelial cells were produced and incubated with AID overexpressed plasmid or chronic inflammatory cytokines. This study suggested that AID and interferon-gamma tended to up-regulate the expression of amelogenin and ameloblastin. These results may imply the possibility that gene mutation on odontogenic epithelial cells by AID causes the abnormal signal transduction and upregulates the expression of ameloblastin, amelogenin and/or erbB.
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Report
(4 results)
Research Products
(1 results)
