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Basic study on bacterial membrane bound peroxidase

Research Project

Project/Area Number 24791971
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

KAWARAI Taketo  日本歯科大学, 生命歯学部, 講師 (90409079)

Co-Investigator(Renkei-kenkyūsha) KONISHI Kiyoshi  日本歯科大学, 生命歯学部, 教授 (20178289)
Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords酵素 / 細菌 / 感染症 / キノールペルオキシダーぜ / 過酸化水素 / Escherichia coli / 侵襲性歯周炎原因菌 / ペルオキシダーゼ / 呼吸鎖
Research Abstract

A membrane-bound enzyme, quinol peroxidase (QPO) that contributes hydrogen peroxide removal and leukotoxin production of Aggregatibacter actinomycetemcomitans, which is an oral pathogen causing localized aggressive periodontitis. In this study, overexpression and partial purification of YhjA, a QPO homologue in Escherichia coli, was constructed. On the other hand, The kinetic analysis characterized ascochlorin and illicicolin B as highly potent inhibitors of QPO and these compounds inhibited QPO in a competitive-type and mixed-type manners, respectively.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (6 results)

All 2013 2012

All Presentation (6 results)

  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼに対する阻害剤2013

    • Author(s)
      河原井武人, 古西清司
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼに対する阻害剤の検討2013

    • Author(s)
      河原井武人, 古西清司
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      横浜
    • Year and Date
      2013-09-13
    • Related Report
      2013 Final Research Report
  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼに対する阻害剤の検討2013

    • Author(s)
      河原井武人, 塩見和朗、大村智、古西清司
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      神奈川県(パシフィコ横浜)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼに対する阻害剤2013

    • Author(s)
      河原井武人, 古西清司
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山県(岡山コンベンションセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼの酵素学的性質2013

    • Author(s)
      河原井武人、古西清司
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      幕張メッセ(千葉)
    • Related Report
      2012 Research-status Report
  • [Presentation] 侵襲性歯周炎原因菌のキノールペルオキシダーゼの酵素学的解析2012

    • Author(s)
      河原井武人、古西清司
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2012 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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