| Project/Area Number |
24791971
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
KAWARAI Taketo 日本歯科大学, 生命歯学部, 講師 (90409079)
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| Co-Investigator(Renkei-kenkyūsha) |
KONISHI Kiyoshi 日本歯科大学, 生命歯学部, 教授 (20178289)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 酵素 / 細菌 / 感染症 / キノールペルオキシダーぜ / 過酸化水素 / Escherichia coli / 侵襲性歯周炎原因菌 / ペルオキシダーゼ / 呼吸鎖 |
|---|
| Research Abstract |
A membrane-bound enzyme, quinol peroxidase (QPO) that contributes hydrogen peroxide removal and leukotoxin production of Aggregatibacter actinomycetemcomitans, which is an oral pathogen causing localized aggressive periodontitis. In this study, overexpression and partial purification of YhjA, a QPO homologue in Escherichia coli, was constructed. On the other hand, The kinetic analysis characterized ascochlorin and illicicolin B as highly potent inhibitors of QPO and these compounds inhibited QPO in a competitive-type and mixed-type manners, respectively.
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