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Major membrane protein TDE2508 regulates adhesive potency in Treponema denticola.

Research Project

Project/Area Number 24791973
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Morphological basic dentistry
Research InstitutionTohoku University (2014)
Aichi Gakuin University (2012-2013)

Principal Investigator

ABIKO YUKI  東北大学, 歯学研究科(研究院), 助教 (00470170)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsTreponema denticola / Treponema denticola / 付着
Outline of Final Research Achievements

In this study, We then analyzed proteins in a membrane fraction in T. denticola and identified 16 major membrane-associated proteins, and characterized one of them, TDE2508, whose biological function was not yet known. Although this protein, which exhibited a complex conformation, was presumably localized in the outer membrane, we did not find conclusive evidence that it was exposed on the cell surface. Intriguingly, a TDE2508-deficient mutant exhibited significantly increased biofilm formation and adherent activity on human gingival epithelial cells. However, the protein deficiency did not alter autoaggregation, coaggregation with Porphyromonas gingivalis, hemagglutination, cell surface hydrophobicity, motility, or expression of Msp which was reported to be an adherent molecule in this bacteria. In conclusion, the major membrane protein TDE2508 regulates biofilm formation and the adhesive potency of T. denticola, although the underlying mechanism remains unclear.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (5 results)

All 2014 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (3 results)

  • [Journal Article] Characterization of Treponema denticola mutants defective in the major antigenic proteins, Msp and TmpC.2014

    • Author(s)
      Abiko Y, Nagano K, Yoshida Y, Yoshimura F.
    • Journal Title

      PLoS One

      Volume: 17;9(11) Issue: 11 Pages: e113565-e113565

    • DOI

      10.1371/journal.pone.0113565

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Major Membrane Protein TDE2508 Regulates Adhesive Potency in Treponema denticola2014

    • Author(s)
      Abiko Y, Nagano K, Yoshida Y, Yoshimura F
    • Journal Title

      PLoS One

      Volume: 21;9(2) Issue: 2 Pages: e89051-e89051

    • DOI

      10.1371/journal.pone.0089051

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 歯周病関連細菌Treponema denticolaの主要膜タンパク質TDE2508の性状および機能解析

    • Author(s)
      安彦友希, 永野恵司, 吉田康夫, 吉村文信
    • Organizer
      第82回学術大会愛知学院大学歯学会
    • Place of Presentation
      愛知学院大学(名古屋)
    • Related Report
      2013 Research-status Report
  • [Presentation] Analysis of major outer membrane protein TDE2508 of Treponema denticola

    • Author(s)
      Abiko Y, Nagano K, Yoshida Y, Yoshimura F
    • Organizer
      第2回IADR Asia Pacific Region 学術大会
    • Place of Presentation
      Plaza Athenee, Bangkok(Thai, Bangkok)
    • Related Report
      2013 Research-status Report
  • [Presentation] 歯周病関連細菌Treponema denticolaの主要膜タンパク質の性状解析

    • Author(s)
      安彦友希, 永野恵司, 吉田康夫, 吉村文信
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山コンベンションセンター(岡山)
    • Related Report
      2013 Research-status Report

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Published: 2013-05-31   Modified: 2019-07-29  

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