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A role of monocarboxylate transporters in osteoporosis caused by diabetics

Research Project

Project/Area Number 24791985
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional basic dentistry
Research InstitutionShowa University

Principal Investigator

YOSHIMURA Kentaro  昭和大学, 歯学部, 助教 (10585699)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords骨芽細胞 / 遺伝子発現 / BMP-2 / MCT-1 / 乳酸 / 糖尿病 / グルコース
Research Abstract

MCT1 is a ubiquitously expressed transmembrane transporter. It transports pyruvate, the terminal product of glycolysis, into mitochondria, resulting in the production of ATP. Introduction of siRNAs for MCT1 strongly suppressed the expression of alkaline phosphatase, an osteoblast differentiation marker, in mouse primary osteoblasts and C2C12 cells exposed to BMP-2. Furthermore, MCT-1 siRNAs suppressed phosphorylation/activation of Smad, a major signaling event after stimulation by BMP-2. In conclusion, MCT-1 not only transports its substrates but also up-regulates osteoblast differentiation by enhanced expression of the genes ruled BMP-2.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (11 results) (of which Invited: 1 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells2013

    • Author(s)
      Shibuya I, Yoshimura K, Miyamoto Y, Yamada A, Takami M, Suzawa T, Suzuki D, Ikumi N, Hiura F, Anada T, Suzuki O, Kamijo R
    • Journal Title

      Cell Tissue Res

      Volume: 352(2) Pages: 401-412

    • Related Report
      2013 Final Research Report
  • [Journal Article] Octacalcium phosphate suppresses chondrogenic differentiation of ATDC5 cells2013

    • Author(s)
      I Shibuya, K Yoshimura, Y Miyamoto, A Yamada, M Takami, T Suzawa, D Suzuki, N Ikumi, F Hiura, T Anada, O Suzuki, R Kamijo.
    • Journal Title

      Cell Tissue Res

      Volume: 352 Issue: 2 Pages: 401

    • DOI

      10.1007/s00441-012-1548-8

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes2013

    • Author(s)
      Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
    • Journal Title

      PLoS ONE

      Volume: Vol.8 Issue: 2 Pages: e56984-e56984

    • DOI

      10.1371/journal.pone.0056984

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nephronectin expression is regulated by SMAD signaling in osteoblast-like MC3T3-E1 cells2012

    • Author(s)
      Tsukasaki M, Yamada A, Yoshimura K, Miyazono A, Yamamoto M, Takami M, Miyamoto Y, Morimura N, Kamijo R
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 425 Issue: 2 Pages: 390-392

    • DOI

      10.1016/j.bbrc.2012.07.106

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via late phase activation of nuclear factor κB and expression of phagocyte-type NADPH oxidase2013

    • Author(s)
      吉村健太郎
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山(第25回歯科基礎医学会賞受賞講演)
    • Related Report
      2013 Final Research Report
  • [Presentation] Monocarboxylate transporter-1 は骨芽細胞におけるアルカリホスファターゼの発現に必要である2013

    • Author(s)
      吉村健太郎, 宮本洋一, 星野真理江, 宮本 尚, 斎藤章大, 船登咲映, 泉田恵理, 槇 宏太郎, 山本松男, 馬場一美, 上條竜太郎
    • Organizer
      日本口腔組織培養学会設立50 周年記念学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Roles of lysine-specific gingipain in osteoclast differentiation induced by inflammatory cytokines2013

    • Author(s)
      Akiyama T, Miyamoto Y, Yamada A, Takami M, Yoshimura K, Hoshino M, Imamura T, Kamijo R, Baba K
    • Organizer
      University of Toronto Research Day
    • Place of Presentation
      Toronto, Canada
    • Related Report
      2013 Final Research Report
  • [Presentation] Monocarboxylate transporter-1は骨芽細胞におけるアルカリホスファターゼの発現に必要である.2013

    • Author(s)
      吉村健太郎、宮本洋一、星野真理江、宮本 尚、斎藤章大、船登咲映、泉田恵理、槇 宏太郎、山本松男、馬場一美、上條竜太郎
    • Organizer
      日本口腔組織培養学会設立50周年記念学術大会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] Monocarboxylate transporter-1 is required for cell death in mouse chondrocytic ATDC5 cells exposed to interleukin-1β via late phase activation of nuclear factor κB and expression of phagocyte-type NADPH oxidase.2013

    • Author(s)
      吉村健太郎
    • Organizer
      第55回歯科基礎医学会学術大会
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Monocarboxylate transporter-1 はBMP-2刺激後のSmad1 のリン酸化に関与することで骨芽細胞分化を正に制御する2012

    • Author(s)
      吉村健太郎, 宮本洋一, 星野真理江, 秋山智人, 塚崎雅之, 山田 篤, 高見正道, 須澤徹夫, 馬場一美, 上條竜太郎
    • Organizer
      第30回日本骨代謝学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] 歯周病原菌由来リシン特異的ジンジパインはオステオプロテゲリンを優先的に分解しTNF-およびIL-1βによる破骨細胞分化を促進する2012

    • Author(s)
      秋山智人, 宮本洋一, 山田 篤, 高見正道, 吉村健太郎, 星野真理江, 宮本 尚, 槇 宏太郎, 馬場一美, 上條竜太郎
    • Organizer
      第54回歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山
    • Related Report
      2013 Final Research Report
  • [Presentation] Importance of proteolytic degradation of osteoprotegerin by lysine-specific gingipain in periodontal osteoclastogenesis2012

    • Author(s)
      Akiyama T, Miyamoto Y, Yamada A, Takami M, Yasuhara R, Yoshimura K, Hoshino M, Maruyama T, Mishima K, Baba K, Kamijo R
    • Organizer
      ASBMR 2012 Annual Meeting
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2013 Final Research Report
  • [Presentation] Monocarboxylate transporter-1はBMP-2刺激後のSmad1のリン酸化に関与することで骨芽細胞分化を正に制御する。2012

    • Author(s)
      吉村健太郎、宮本洋一、星野真理江、秋山智人、塚崎雅之、山田篤、高見正道、須澤徹夫、馬場一美、上條竜太郎
    • Organizer
      第30回日本骨代謝学会学術集会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] Importance of proteolytic degradation of osteoprotegerin by lysine-specific gingipain in periodontal osteoclastogenesis.2012

    • Author(s)
      Akiyama T, Miyamoto Y, Yamada A, Takami M, Yasuhara R, Yoshimura K, Hoshino M, Maruyama T, Mishima K, Baba K, Kamijo R
    • Organizer
      ASBMR 2012 Annual Meeting
    • Place of Presentation
      Minneapolis, MN, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] 歯周病源菌由来リシン特異的ジンジパインはオステオプロテゲリンを優先的に分解しTNF-αおよびIL-1βによる破骨細胞分化を促進する。2012

    • Author(s)
      秋山智人、宮本洋一、山田篤、高見正道、吉村健太郎、星野真理江、宮本尚、槇宏太郎、馬場一美、上條竜太郎
    • Organizer
      第54回歯科基礎医学会学術大会・総会
    • Place of Presentation
      郡山
    • Related Report
      2012 Research-status Report
  • [Book] アンチエイジングシリーズ3 骨研究最前線~代謝・疾病のメカニズムから再生医療・創薬・リハビリ機器・機能性食品開 発まで~, 第3 編 第5 章 第3 節「骨代謝疾患から歯科治療へのアプローチ」2013

    • Author(s)
      吉村健太郎, 宮本洋一, 上條竜太郎
    • Publisher
      (株)エヌ.ティー.エス出版
    • Related Report
      2013 Final Research Report
  • [Book] アンチエイジングシリーズ3 骨研究最前線~代謝・疾病のメカニズムから再生医療・創薬・リハビリ機器・機能性食品開 発まで~、 第3編 第5章 第3節「骨代謝疾患から歯科治療へのアプローチ」2013

    • Author(s)
      吉村健太郎、宮本洋一、上條竜太郎
    • Total Pages
      458
    • Publisher
      (株)エヌ.ティー.エス出版
    • Related Report
      2013 Annual Research Report
  • [Remarks] 昭和大学歯学部口腔生化学講座ホームページ

    • URL

      http://www10.showa-u.ac.jp/~oralbio/

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

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