| Project/Area Number |
24791985
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Showa University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | 骨芽細胞 / 遺伝子発現 / BMP-2 / MCT-1 / 乳酸 / 糖尿病 / グルコース |
|---|
| Research Abstract |
MCT1 is a ubiquitously expressed transmembrane transporter. It transports pyruvate, the terminal product of glycolysis, into mitochondria, resulting in the production of ATP. Introduction of siRNAs for MCT1 strongly suppressed the expression of alkaline phosphatase, an osteoblast differentiation marker, in mouse primary osteoblasts and C2C12 cells exposed to BMP-2. Furthermore, MCT-1 siRNAs suppressed phosphorylation/activation of Smad, a major signaling event after stimulation by BMP-2. In conclusion, MCT-1 not only transports its substrates but also up-regulates osteoblast differentiation by enhanced expression of the genes ruled BMP-2.
|
