Does aquapoirn-6 influx anion in immature secretory granules during maintainance of acidic pH?
| Project/Area Number |
24791986
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional basic dentistry
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| Research Institution | Showa University (2013-2014)
Nihon University (2012) |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 唾液腺 / 分泌顆粒 / 分泌顆粒成熟 / 酸性オルガネラ / 陰イオンチャネル / アクアポリン / 遺伝子サイレンシング / 融合タンパク質 / 耳下腺 / 細胞内小器官 / 口腔生理学 |
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| Outline of Final Research Achievements |
Aquaporin-6(AQP6) is an acid-activated, anion permeated member of aquaporin water channel familiy. We hypothesized that AQP6 is activated in immature secretory granules of salivary gland acinar cells which is maintained with acidic condition while becoming mature state. To determine this hypothesis, we synthesized secretory granules-specific pH indicator and applied to primary salivary gland cells. Newly synthesized pH indicator clealy showed inside pH of salivary secretry granules, however, acidic granules were not shown with pH indicator. On the other hand, AQP6 was accumulated in immature granule membranes rather than mature granule membranes detected with Western blotting of isolated salivary granule membranes. These result suggest that AQP6 might important role of maturation in salivary gland cells.
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Report
(4 results)
Research Products
(8 results)
